Human/Mouse CD44 Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB6127G-025
FAB6127G-100
Detection of CD44 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (3)
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Human/Mouse CD44 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CD44 in flow cytometry.
Source
Recombinant Monoclonal Rat IgG2B Clone # IM7.8.1R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Myeloid leukemia M1 cells induced with Dexamethasone
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD44 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD44 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB6127G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CD44 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB6127G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD44

CD44, also known as ECMR-III, Hermes Antigen, Pgp1, Ly-24, GP90, and HUTCH-I, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interaction through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagen, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44. In the ECD, human and mouse CD44 share about 65% aa sequence identity.

Entrez Gene IDs
960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names
CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human/Mouse CD44 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Latent cellular analysis robustly reveals subtle diversity in large-scale single-cell RNA-seq data
    Authors: C Cheng, J Easton, C Rosencranc, Y Li, B Ju, J Williams, HL Mulder, Y Pang, W Chen, X Chen
    Nucleic Acids Res., 2019-12-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. PTH1-34 improves bone healing by promoting angiogenesis and facilitating MSCs migration and differentiation in a stabilized fracture mouse model
    Authors: X Jiang, C Xu, H Shi, Q Cheng
    PLoS ONE, 2019-12-10;14(12):e0226163.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. siRNA-encapsulated immunoliposomes conjugated with CD44 antibodies target and eliminate gastric cancer-initiating cells
    Authors: F Yang, Z Zheng, L Zheng, J Qin, H Li, X Xue, J Gao, G Fang
    Onco Targets Ther, 2018-10-11;11(0):6811-6825.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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