Human/Mouse CD44 Antibody

Catalog #: MAB6127
6 Citations Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB6127
MAB6127-SP

Detection of CD44 in Human PBMCs by Flow Cytometry.

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All CD44 Antibodies

Product Details

Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse CD44 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse CD44 in flow cytometry.

Source

Recombinant Monoclonal Rat IgG Clone # IM7.8.1R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Myeloid leukemia M1 cells induced with Dexamethasone

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-reported

Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD44 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Flow Cytometry

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Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD44

CD44, also known as ECMR-III, Hermes, Pgp1, Ly-24, GP90, or HUTCH, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44.

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Alternate Names

CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse CD44 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain
Authors: Wang, M;Zhang, L;Novak, SW;Yu, J;Gallina, IS;Xu, LL;Lim, CK;Fernandes, S;Shokhirev, MN;Williams, AE;Saxena, MD;Coorapati, S;Parylak, SL;Quintero, C;Molina, E;Andrade, LR;Manor, U;Gage, FH;
Nature biotechnology
Species: Human hepegivirus, Mollusc
Sample Types: Organoid, Whole Tissue
Applications: Immunohistochemistry
siRNA-encapsulated immunoliposomes conjugated with CD44 antibodies target and eliminate gastric cancer-initiating cells
Authors: F Yang, Z Zheng, L Zheng, J Qin, H Li, X Xue, J Gao, G Fang
Onco Targets Ther, 2018-10-11;11(0):6811-6825.
Species: Human
Sample Types: Whole Cells
Applications: Complex Application
Combination of CD40 agonism and CSF-1R blockade reconditions tumor-associated macrophages and drives potent antitumor immunity
Authors: KR Wiehagen, NM Girgis, DH Yamada, AA Smith, SR Chan, IS Grewal, M Quigley, RI Verona
Cancer Immunol Res, 2017-11-02;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Nicotinamide metabolism regulates glioblastoma stem cell maintenance
Authors: J Jung, LJY Kim, X Wang, Q Wu, T Sanvoranar, CG Hubert, BC Prager, LC Wallace, X Jin, SC Mack, JN Rich
JCI Insight, 2017-05-18;2(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
Stem Cells Int, 2016-10-20;2016(0):6104780.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Crucial role of hyaluronan in neointimal formation after vascular injury.
Authors: Kashima Y, Takahashi M, Shiba Y, Itano N, Izawa A, Koyama J, Nakayama J, Taniguchi S, Kimata K, Ikeda U
PLoS ONE, 2013-03-06;8(3):e58760.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization