Human/Mouse COX-2 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse COX‑2 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cell (PBMC) and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 ug/mL LPS for 24 hours and U937 human histiocytic lymphoma cell line untreated or treated with 100 nM PMA and 1 ug/mL LPS for 48 hours and 24 hours, respectively. PVDF membrane was probed with 1 µg/mL of Human/Mouse COX-2 Polyclonal Antibody (Catalog # AF4198), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for COX-2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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COX‑2 in HUVEC Human Cells. COX-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Human/Mouse COX-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4198) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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COX‑2 in RAW 264.7 Mouse Cells. COX-2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cells stimulated with LPS using Goat Anti-Human/Mouse COX-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4198) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of COX‑2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line treated with 1 µg/mL LPS for 24 hours was stained with Goat Anti-Human/Mouse COX-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4198, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: COX-2
Cyclooxygenase-2 (COX-2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX-2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H 2. Mature human COX-2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18-55), a potential membrane interacting region (aa 277-292) and a globular catalytic domain (aa 293-604). At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids. Mature human COX-2 shows 87% aa identity to mouse COX-2.
Product Datasheets
Citations for Human/Mouse COX-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice
Authors: J Liu, S Lane, R Lall, M Russo, L Farrell, M Debreli Co, C Curtin, R Araujo-Gut, M Scherrer-C, BH Trachtenbe, J Kim, E Tolosano, A Ghigo, RE Gerszten, A Asnani
Science Advances, 2022-12-23;8(51):eadc9245.
Species: Mouse
Sample Types: Tissue Lysate
Applications: Western Blot -
Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
Oncogene, 2022-06-14;0(0):.
Species: Xenograft
Sample Types: Tissue Homogenates
Applications: Capillary Immunoblot -
The inhibitory effects of Orengedokuto on inducible PGE2 production in BV-2 microglial cells
Authors: Y Iwata, M Miyao, A Hirotsu, K Tatsumi, T Matsuyama, N Uetsuki, T Tanaka
Heliyon, 2021-08-11;7(8):e07759.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
TET2 Regulates the Neuroinflammatory Response in Microglia
Authors: A Carrillo-J, Ö Deniz, MV Niklison-C, R Ruiz, K Bezerra-Sa, V Stratoulia, R Amouroux, PK Yip, A Vilalta, M Cheray, AM Scott-Eger, E Rivas, K Tayara, I García-Dom, J Garcia-Rev, JC Fernandez-, AM Espinosa-O, X Shen, P St George-, GC Brown, P Hajkova, B Joseph, JL Venero, MR Branco, MA Burguillos
Cell Rep, 2019-10-15;29(3):697-713.e8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Response gene to complement 32 expression in macrophages augments paracrine stimulation-mediated colon cancer progression
Authors: P Zhao, B Wang, Z Zhang, W Zhang, Y Liu
Cell Death Dis, 2019-10-10;10(10):776.
Species: Human, Xenograft
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Multi-Staged Regulation of Lipid Signaling Mediators during Myogenesis by COX-1/2 Pathways
Authors: C Mo, Z Wang, L Bonewald, M Brotto
Int J Mol Sci, 2019-09-04;20(18):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Increased Toxoplasma gondii Intracellular Proliferation in Human Extravillous Trophoblast Cells (HTR8/SVneo Line) Is Sequentially Triggered by MIF, ERK1/2, and COX-2
Authors: ICB Milian, RJ Silva, C Manzan-Mar, BF Barbosa, PM Guirelli, M Ribeiro, A de Oliveir, F Ietta, JR Mineo, P Silva Fran, EAV Ferro
Front Microbiol, 2019-04-24;10(0):852.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Stromal versus tumoral inflammation differentially contribute to metastasis and poor survival in laryngeal squamous cell carcinoma
Authors: B Höing, O Kanaan, P Altenhoff, R Petri, K Thangavelu, A Schlüter, S Lang, A Bankfalvi, S Brandau
Oncotarget, 2018-01-03;9(9):8415-8426.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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