Human/Mouse NGFR/TNFRSF16 Biotinylated Antibody

Catalog #: BAF367 Datasheet / COA / SDS

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BAF367

Human/Mouse/Rat NGF R/TNFRSF16 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NGFR/TNFRSF16 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse NGF R/TNFRSF16 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NGF R/TNFRSF16
Lys29-Asn250
Accession # P08138

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human NGF R/TNFRSF16 Fc Chimera (Catalog # 367-NR)

Human NGF R Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human NGFR/TNFRSF16 Antibody (Catalog # AF367)

Standard: Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein (Catalog # 367-NR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA Detection (Matched Antibody Pair)

Human/Mouse/Rat NGF R/TNFRSF16 Antibody in ELISA Standard Curve.

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Human/Mouse/Rat NGF R/TNFRSF16 ELISA Standard Curve. Recombinant Human/Mouse/Rat NGF R/TNFRSF16 (Catalog # 367-NR) was serially diluted 2-fold and captured by Goat Anti-Human/Mouse/Rat NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF367) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse NGF R/TNFRSF16 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF367) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGFR/TNFRSF16

p75 neurotrophin receptor, also named low affinity NGF receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3 and NT-4 approximately equally with low affinity. The cytoplasmic region contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and rats.

References

Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.

Long Name

Nerve Growth Factor Receptor

Entrez Gene IDs

4804 (Human); 18053 (Mouse); 117089 (Rat)

Alternate Names

CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16