Human/Mouse/Rat Musashi-1 Antibody

Catalog #: AF2628
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2628
AF2628-SP

Detection of Recombinant Human Musashi‑1 by Western Blot.

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Citations (6)

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Human/Mouse/Rat Musashi-1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Musashi-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Musashi‑2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Musashi-1
Met1-His362
Accession # O43347

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

1-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Recombinant Human Musashi‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human Musashi-1 and Recombinant Human Musashi-2. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/ Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Musashi-1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry

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Musashi‑1 in Mouse Cortical Stem Cells. Musashi-1 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry

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Musashi‑1 in Rat Cortical Stem Cells. Musashi-1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry

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Musashi‑1 in Human Intestine. Musashi-1 was detected in immersion fixed paraffin-embedded sections of human intestine using 1 µg/mL Goat Anti-Human/Mouse/ Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Musashi-1

Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).

References

Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.

Entrez Gene IDs

4440 (Human)

Alternate Names

MSI1; Musashi (Drosophila) homolog 1; musashi homolog 1 (Drosophila); Musashi1; Musashi-1; RNA-binding protein Musashi homolog 1

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Product Datasheet

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Related Research Areas

Citations for Human/Mouse/Rat Musashi-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Musashi-2 (MSI2) regulation of DNA damage response in lung cancer
Authors: Bychkov, I;Deneka, A;Topchu, I;Pangeni, RP;Lengner, C;Karanicolas, J;Golemis, EA;Makhov, P;Boumber, Y;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Spatial Distribution of Macrophages During Callus Formation and Maturation Reveals Close Crosstalk Between Macrophages and Newly Forming Vessels
Authors: Jonathan Stefanowski, Annemarie Lang, Ariana Rauch, Linus Aulich, Markus Köhler, Alexander F. Fiedler et al.
Frontiers in Immunology
Rapid generation of OPC-like cells from human pluripotent stem cells for treating spinal cord injury
Authors: DS Kim, SJ Jung, JS Lee, BY Lim, HA Kim, JE Yoo, DW Kim, JW Leem
Exp. Mol. Med., 2017-07-28;49(7):e361.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Knockout of RNA Binding Protein MSI2 Impairs Follicle Development in the Mouse Ovary: Characterization of MSI1 and MSI2 during Folliculogenesis
Authors: Jessie M. Sutherland, Alexander P. Sobinoff, Kara M. Gunter, Barbara A. Fraser, Victoria Pye, Ilana R. Bernstein et al.
Biomolecules
Expression and regulation of prostate apoptosis response-4 (Par-4) in human glioma stem cells in drug-induced apoptosis.
Authors: Jagtap, Jayashre, Dawood, Parveen, Shah, Reecha D, Chandrika, Goparaju, Natesh, Kumar, Shiras, Anjali, Hegde, Amba S, Ranade, Deepak, Shastry, Padma
PLoS ONE, 2014-02-11;9(2):e88505.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Side populations of gastrointestinal cancers are not enriched in stem cells.
Authors: Burkert J, Otto WR, Wright NA
J. Pathol., 2008-04-01;214(5):564-73.
Species: Human
Sample Types: Whole Cells
Applications: ICC