Human Nestin APC-conjugated Antibody

Catalog #: IC1259A
2 Citations Datasheet / COA / SDS

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IC1259A

Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry.

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All Nestin Antibodies

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Citations (2)

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Human Nestin APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nestin in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 196908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Nestin
Accession # P48681

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Nestin antibody in NTera-2 Human Cell Line antibody by Flow Cytometry.

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Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Nestin APC-conjugated Monoclonal Antibody (Catalog # IC1259A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Nestin

Nestin is a class VI intermediate filament protein that is expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells. Its transient expression is a critical step in both the neural differentiation pathway and that of CNS glia. Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors and hematopoietic progenitors. Full-length human Nestin shares 51% amino acid sequence identity to mouse Nestin.

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Alternate Names

FLJ21841; NES; Nestin

Product Datasheets

Product Datasheet

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Citations for Human Nestin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The STEMRI trial: Magnetic resonance spectroscopy imaging can define tumor areas enriched in glioblastoma stem-like cells
Authors: Lemarié, A;Lubrano, V;Delmas, C;Lusque, A;Cerapio, JP;Perrier, M;Siegfried, A;Arnauduc, F;Nicaise, Y;Dahan, P;Filleron, T;Mounier, M;Toulas, C;Cohen-Jonathan Moyal, E;
Science advances
Species: Human
Sample Types: Whole Tissue
Applications: Flow Cytometry
Chemokine receptor CXCR3 promotes growth of glioma.
Authors: Liu C, Luo D, Reynolds BA
Carcinogenesis, 2010-11-03;32(2):129-37.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry