Human Neurofascin Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB8208N Datasheet / COA / SDS

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FAB8208N-100UG

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Human Neurofascin Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neurofascin in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1018843

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human Neurofascin
Ile25-Trp1039
Accession # NP_055905

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

U87-MG human cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Neurofascin

Neurofascin, a type I transmembrane glycoprotein, is a member of the L1 family of cell adhesion molecules (CAMs) (1-4). L1CAM family members are composed of an extracellular domain (ECD) that contains six immunoglobulin (Ig)-like domains and multiple fibronectin type III repeats, followed by transmembrane and cytoplasmic domains (2, 4, 5). Multiple isoforms of Neurofascin, including NF155, NF166, NF180, and NF186, can be generated by alternative splicing with a predicted range of approximately 70 kDa to 150 kDa (4). These isoforms differ in the combination of fibronectin type III repeats, as well as in the presence of a proline-, alanine-, and threonine-rich segment (PAT domain) located just after the fourth fibronectin type III repeat (4). This recombinant human Neurofascin protein corresponds to rat isoform NF155 and shares 96% amino acid sequence identity with comparable regions of rat and mouse Neurofascin. In rats, NF155 is transiently expressed by oligodendrocytes and Schwann cells during axon myelination (6, 7). NF155 clusters in paranodal regions of oligodendroglia and binds to the Caspr-Contactin complex located on the adjacent axon to form and stabilize paranodal axoglial junctions (8-11). It has been suggested that the ECD of NF155 must be cleaved from oligodendroglia membranes to form and/or stabilize the paranodal structure (12). NF155 has also been shown to promote neuronal adhesion and neurite outgrowth in rats and chickens (12-14). Alterations in NF155 expression have been associated with multiple sclerosis (15).

References

Rathjen, F.G. et al. (1987) Cell 51:841.
Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
Kriebel, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:694.
Liu, H. et al. (2011) J. Biol. Chem. 286:797.
Collinson, J.M. et al. (1998) Glia 23:11.
Basak, S. et al. (2007) Dev. Biol. 311:408.
Charles, P. et al. (2002) Curr. Biol. 12:217.
Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
Sherman, D.L. et al. (2005) Neuron 48:737.
Maier, O. et al. (2006) Exp. Cell Res. 312:500.
Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
Howell, O.W. et al. (2006) Brain 129:3173.

Entrez Gene IDs

23114 (Human); 269116 (Mouse); 116690 (Rat)

Alternate Names

DKFZp686P2250; KIAA0756FLJ46866; neurofascin homolog (chicken); neurofascin homolog; Neurofascin; NF; NFASC; NRCAML

Product Datasheets

Product Datasheet

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.