Human NG2/MCSP Antibody

Name: Human NG2/MCSP Antibody
Brand: R&D Systems
SKU: MAB2585
Rating: 5 (2 reviews)

Catalog #: MAB2585
10 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2585
MAB2585-SP

Detection of Human NG2/MCSP by Western Blot.

2 Images

All NG2/MCSP Antibodies

Product Details

Citations (10)

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP Antibody Summary

Species Reactivity

Human

Specificity

Detects human NG2/MCSP in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # LHM-2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human melanoma cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

G361 human melanoma cell line (Smith, F.O. et al. (1996) Blood 87:1123).

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human NG2/MCSP by Western Blot. Western blot shows lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

NG2/MCSP in Human Skin Cancer Tissue. NG2/MCSP was detected in immersion fixed paraffin-embedded sections of human skin cancer tissue (melanoma) using 10 µg/mL Mouse Anti-Human NG2/MCSP Monoclonal Antibody (Catalog # MAB2585) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References

Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
Legg, J. et al. (2003) Development 130:6049.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Alternate Names

AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human NG2/MCSP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma
Authors: J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau
Sci Adv, 2020-02-26;6(9):eaax3223.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot
Amyloid-beta 1-40 is associated with alterations in NG2+ pericyte population exï¿½vivo and inï¿½vitro
Authors: N Schultz, K Brännström, E Byman, S Moussaud, HM Nielsen, A Olofsson, M Wennström
Aging Cell, 2018-02-17;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Equine Mesenchymal Stromal Cells retain a pericyte-like phenotype
Authors: CL Esteves, TA Sheldrake, L Dawson, T Menghini, E Rink, K Amilon, N Khan, B Peault, X Donadeu
Stem Cells Dev., 2017-05-09;0(0):.
Species: Equine
Sample Types: Whole Cells
Applications: ICC
Isolation and characterization of equine native MSC populations
Authors: CL Esteves, TA Sheldrake, SP Mesquita, JJ Pesántez, T Menghini, L Dawson, B Péault, FX Donadeu
Stem Cell Res Ther, 2017-04-18;8(1):80.
Species: Equine
Sample Types: Whole Tissue
Applications: IHC
Dual targeting NG2 and GD3A using Mab-Zap immunotoxin results in reduced glioma cell viability in vitro.
Authors: Higgins S, Fillmore H, Ashkan K, Butt A, Pilkington G
Anticancer Res, 2015-01-01;35(1):77-84.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Expression of the chondroitin sulphate proteoglycan, NG2, in paediatric brain tumors.
Authors: Higgins S, Bolteus A, Donovan L, Hasegawa H, Doey L, Al Sarraj S, King A, Ashkan K, Roncaroli F, Fillmore H, Pilkington G
Anticancer Res, 2014-12-01;34(12):6919-24.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-P
Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability.
Authors: Davaine J, Quillard T, Brion R, Laperine O, Guyomarch B, Merlini T, Chatelais M, Guilbaud F, Brennan M, Charrier C, Heymann D, Goueffic Y, Heymann M
PLoS ONE, 2014-09-26;9(9):e107642.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Chondroitin sulfate proteoglycan CSPG4 as a novel hypoxia-sensitive marker in pancreatic tumors.
Authors: Keleg S, Titov A, Heller A, Giese T, Tjaden C, Ahmad S, Gaida M, Bauer A, Werner J, Giese N
PLoS ONE, 2014-06-16;9(6):e100178.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-P
Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
Nature, 2013-04-24;497(7449):369-73.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Two distinct types of blood vessels in clear cell renal cell carcinoma have contrasting prognostic implications.
Authors: Yao X, Qian CN, Zhang ZF, Tan MH, Kort EJ, Yang XJ, Resau JH, Teh BT
Clin. Cancer Res., 2007-01-01;13(1):161-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P