Human Nox4 Antibody

Catalog #: AF8158
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8158
AF8158-SP

Detection of Human Nox4 by Western Blot.

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All Nox4 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nox4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nox4 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Nox4
Lys450-Ser578
Accession # Q9NPH5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Nox4 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Nox4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8158) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nox4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nox4

NADPH Oxidase 4/NOX4, also known as Renal NADPH Oxidase/RENOX and Kidney Oxidase 1/KOX1, is a 66 to 75 kDa member of the NOX family of NADPH oxidases. NOX family members are a major source of reactive oxygen species (ROS), including hydrogen peroxide and superoxide, and are critical mediators of redox signaling. NOX4 is strongly expressed in the kidney, especially in the proximal convoluted tubule cells of the renal cortex, and may function as an oxygen sensor that regulates the synthesis of erythropoietin. Expression is also high in endothelial cells, and because aortas from deficient mice exhibit increased inflammation, hypertrophy and endothelial dysfunction, NOX4 may function to protect the vasculature during ischemic or inflammatory stress. Human NOX4 is 578 amino acids (aa) in length, with several additional smaller isoforms identified. Over aa 450-578, human NOX4 has 92% sequence identity with mouse NOX4, and 91% identity with rat NOX4.

Long Name

NADPH Oxidase 4

Entrez Gene IDs

50507 (Human); 50490 (Mouse); 85431 (Rat)

Alternate Names

EC 1.6.3; Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX; KOX-1; KOX-1Renal NAD(P)H-oxidase; NADPH oxidase 4; Nox4; RENOX; RENOXEC 1.6.3.-

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Nox4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Modulation of H4K16Ac levels reduces pro-fibrotic gene expression and mitigates lung fibrosis in aged mice
Authors: X Zhang, H Liu, JQ Zhou, S Krick, JW Barnes, VJ Thannickal, YY Sanders
Theranostics, 2022-01-01;12(2):530-541.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Brd4-p300 inhibition down-regulates Nox4 and accelerates lung fibrosis resolution in aged mice
Authors: YY Sanders, X Lyu, QJ Zhou, Z Xiang, D Stanford, S Bodduluri, SM Rowe, VJ Thannickal
JCI Insight, 2020-07-23;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot