Human PAM Antibody

Catalog #: AF4837
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4837
AF4837-SP

All PAM Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PAM Antibody Summary

Species Reactivity

Human

Specificity

Detects human PAM in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PAM
Phe21-Val817
Accession # P19021

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human PAM

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAM

PAM (Peptidyl-Gly alpha -amidating monooxygenase) is a dual-function, 95‑110 kDa protein that belongs to both the lyase and monooxygenase enzyme families. It is expressed in a variety of endocrine and exocrine glands and catalyzes the conversion of C‑terminal Gly into amidated residues. Human ProPAM is a 953 amino acid (aa) type I transmembrane glycoprotein. It contains an 833 aa extracellular domain (ECD) (aa 31‑863) and an 86 aa cytoplasmic tail. The ECD shows one PHM/monooxygenase region (aa 40‑347) and a PAL/lyase domain (aa 495‑817). Both regions are enzymatically active. Alternative splicing may generate a soluble form (aa 31‑828), and remove aa 388‑494 that link the two embedded enzyme segments. Over aa 21‑817, human PAM is 91% aa identical to mouse PAM.

Long Name

Peptidylglycine Alpha-Amidating Monooxygenase

Entrez Gene IDs

5066 (Human); 18484 (Mouse); 25508 (Rat)

Alternate Names

PAL; PAM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylamidoglycolate lyase; peptidylglycine 2-hydroxylase; peptidyl-glycine alpha-amidating monooxygenase; peptidylglycine alpha-amidating monooxygenase; peptidylglycine alpha-hydroxylating monooxygenase; PHM

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human PAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

PAM staining intensity of primary neuroendocrine neoplasms is a potential prognostic biomarker
Authors: TM Horton, V Sundaram, CH Lee, K Hornbacker, A Van Vleck, KN Benjamin, A Zemek, TA Longacre, PL Kunz, JP Annes
Sci Rep, 2020-07-02;10(1):10943.
Species: Human
Sample Types: Whole Tissue
Applications: IHC