Home / PDGF R alpha / Human PDGF R alpha Antibody

Human PDGF R alpha Antibody

Catalog #: AF-307-NA
28 Citations 2 Reviews Datasheet / COA / SDS
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AF-307-NA
AF-307-SP
Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody.
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Product Details
Citations (28)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse PDGF R alpha, recombinant human (rh) PDGF R beta, rhFGF R2, and rhFGF R3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PDGF R alpha
Gln24-Glu524
Accession # P16234
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PDGF R alpha (Catalog # 322-PR)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize PDGF‑AA-induced proliferation in the WS-1 human fetal skin fibroblast cell line. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. View Larger

Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody. Recombinant Human PDGF-AA (Catalog # 221-AA) stimulates proliferation in the WS-1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA). The ND50 is typically 1-6 µg/mL.

Immunohistochemistry PDGF Ra antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PDGF R alpha in Human Breast Cancer Tissue. PDGF Ra was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry PDGF Ra antibody in Human Ovary by Immunohistochemistry (IHC-P). View Larger

PDGF R alpha in Human Ovary. PDGF Ra was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry PDGF Ra antibody in Human Osteosarcoma by Immunohistochemistry (IHC-P). View Larger

PDGF R alpha in Human Osteosarcoma. PDGF Ra was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Goat Anti-Human PDGF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF‑AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB and -AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell, and liver endothelial cells. Soluble PDGF R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

References
  1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

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Citations for Human PDGF R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patients
    Authors: L Di Pietro, F Giacalone, E Ragozzino, V Saccone, F Tiberio, M De Bardi, M Picozza, G Borsellino, W Lattanzi, E Guadagni, S Bortolani, G Tasca, E Ricci, O Parolini
    Cell Death & Disease, 2022-09-16;13(9):793.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes
    Authors: A Wrona, V Aleksandro, T Bereza, P Basta, A Gil, M Ulatowska-, M Mazur-Lask, K Pity?ski, K Gil
    International Journal of Molecular Sciences, 2022-05-31;23(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Morphologic evidence of telocytes in human thyroid stromal tissue
    Authors: I Rosa, L Ibba-Manne, D Guasti, G Perigli, MS Faussone-P, M Manetti
    Journal of Cellular and Molecular Medicine, 2022-03-20;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Deciphering the spatial-temporal transcriptional landscape of human hypothalamus development
    Authors: X Zhou, Y Lu, F Zhao, J Dong, W Ma, S Zhong, M Wang, B Wang, Y Zhao, Y Shi, Q Ma, T Lu, J Zhang, X Wang, Q Wu
    Cell Stem Cell, 2021-12-07;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. IL-1-driven stromal-neutrophil interactions define a subset of patients with inflammatory bowel disease that does not respond to therapies
    Authors: M Friedrich, M Pohin, MA Jackson, I Korsunsky, SJ Bullers, K Rue-Albrec, Z Christofor, D Sathananth, T Thomas, R Ravindran, R Tandon, RS Peres, H Sharpe, K Wei, GFM Watts, EH Mann, A Geremia, M Attar, Oxford IBD, Roche Fibr, S McCuaig, L Thomas, E Collantes, HH Uhlig, SN Sansom, A Easton, S Raychaudhu, SP Travis, FM Powrie
    Nature Medicine, 2021-10-21;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Oviductal Telocytes in Patients with Uterine Myoma
    Authors: V Aleksandro, A Wrona, T Bereza, K Pity?ski, K Gil
    Biomedicines, 2021-08-20;9(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Single-nucleus chromatin accessibility and transcriptomic characterization of Alzheimer's disease
    Authors: S Morabito, E Miyoshi, N Michael, S Shahin, AC Martini, E Head, J Silva, K Leavy, M Perez-Rose, V Swarup
    Nature Genetics, 2021-07-08;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity
    Authors: P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen
    JCI Insight, 2021-05-10;6(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Heterogeneity of glial progenitor cells during the neurogenesis-to-gliogenesis switch in the developing human cerebral cortex
    Authors: Y Fu, M Yang, H Yu, Y Wang, X Wu, J Yong, Y Mao, Y Cui, X Fan, L Wen, J Qiao, F Tang
    Cell Reports, 2021-03-02;34(9):108788.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles
    Authors: J Just, Y Yan, J Farup, P Sieljacks, M Sloth, M Venø, T Gu, FV de Paoli, JR Nyengaard, R Bæk, MM Jørgensen, J Kjems, K Vissing, KR Drasbek
    Sci Rep, 2020-04-03;10(1):5835.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Patch repair of deep wounds by mobilized fascia
    Authors: D Correa-Gal, D Jiang, S Christ, P Ramesh, H Ye, J Wannemache, S Kalgudde G, Q Yu, M Aichler, A Walch, U Mirastschi, T Volz, Y Rinkevich
    Nature, 2019-11-27;0(0):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  12. Induction of nuclear protein-1 by thyroid hormone enhances platelet-derived growth factor A mediated angiogenesis in liver cancer
    Authors: CY Chen, SM Wu, YH Lin, HC Chi, SL Lin, CT Yeh, WY Chuang, KH Lin
    Theranostics, 2019-04-13;9(8):2361-2379.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Morphological evidence of telocytes in human synovium
    Authors: I Rosa, M Marini, D Guasti, L Ibba-Manne, M Manetti
    Sci Rep, 2018-02-26;8(1):3581.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
    Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
    PLoS ONE, 2017-08-14;12(8):e0182265.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Adaptive changes of telocytes in the urinary bladder of patients affected by neurogenic detrusor overactivity
    Authors: C Traini, MS Fausssone-, D Guasti, G Del Popolo, J Frizzi, S Serni, MG Vannucchi
    J. Cell. Mol. Med., 2017-08-07;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Telocytes in normal and keratoconic human cornea: an immunohistochemical and transmission electron microscopy study
    Authors: M Marini, R Mencucci, I Rosa, E Favuzza, D Guasti, L Ibba-Manne, M Manetti
    J. Cell. Mol. Med., 2017-07-17;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Non-glycanated Decorin Is a Drug Target on Human Adipose Stromal Cells
    Authors: AC Daquinag, A Dadbin, B Snyder, X Wang, AA Sahin, NT Ueno, MG Kolonin
    Mol Ther Oncolytics, 2017-05-17;6(0):1-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. ACL injury reduces satellite cell abundance and promotes fibrogenic cell expansion within skeletal muscle
    Authors: Christopher S Fry
    J. Orthop. Res, 2017-01-15;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Identification and characterization of PDGFRalpha+ mesenchymal progenitors in human skeletal muscle.
    Authors: Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Nakatani M, Morita M, Yamaguchi A, Yamada H, Nishino I, Hamada Y, Tsuchida K
    Cell Death Dis, 2014-04-17;5(0):e1186.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Telocytes in Crohn's disease.
    Authors: Milia A, Ruffo M, Manetti M, Rosa I, Conte D, Fazi M, Messerini L, Ibba-Manneschi L
    J Cell Mol Med, 2013-11-19;17(12):1525-36.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells.
    Authors: Kusuhara, Sentaro, Fukushima, Yoko, Fukuhara, Shigetom, Jakt, Lars Mar, Okada, Mitsuhir, Shimizu, Yuri, Hata, Masayuki, Nishida, Kohji, Negi, Akira, Hirashima, Masanori, Mochizuki, Naoki, Nishikawa, Shin-Ich, Uemura, Akiyoshi
    PLoS ONE, 2012-09-21;7(9):e45858.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection.
    Authors: Soroceanu L, Akhavan A, Cobbs CS
    Nature, 2008-08-13;455(7211):391-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  23. Distinctions between fetal and adult human platelet-derived growth factor-responsive neural precursors.
    Authors: Chojnacki A, Kelly JJ, Hader W, Weiss S
    Ann. Neurol., 2008-08-01;64(2):127-42.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  24. Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor.
    Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A
    Cancer Res., 2007-01-15;67(2):555-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
    Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
    Blood, 2005-12-01;107(5):1896-902.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Immunoprecipitation
  26. Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas.
    Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K
    Cancer Res., 2005-08-15;65(16):7241-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunodepletion, Immunoprecipitation
  27. Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
    Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
    Am. J. Pathol., 2003-09-01;163(3):869-78.
    Species: Rabbit
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Content and activity of cAMP response element-binding protein regulate platelet-derived growth factor receptor-alpha content in vascular smooth muscles.
    Authors: Watson PA, Vinson C, Nesterova A, Reusch JE
    Endocrinology, 2002-08-01;143(8):2922-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human PDGF R alpha Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human PDGF R alpha Antibody
By Anonymous on 04/10/2019
Application: In our hands appears as a duplet Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
Human PDGF R alpha Antibody
By Juwen DuBois on 06/13/2017
Application: ICC/IF Sample Tested: Brain tissue,Spinal cord Species: Mouse