Home / IGF-I R/IGF1R / Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

Catalog #: DYC1770-2
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC1770E
DYC1770-5
DYC1770-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
R&D Systems DuoSet ELISAs
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human IGF-I R in cell lysates. An immobilized capture antibody specific for IGF-I R binds both phosphorylated and unphosphorylated IGF-I R. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues.

IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment segment and a 124 aa cytoplasmic tail. IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, It would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes.

The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligandbinding alpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF-I R stimulates intrinsic kinase activity.

Long Name:
Insulin-like Growth Factor I Receptor
Entrez Gene IDs:
3480 (Human)
Alternate Names:
CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

Citations for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Enhanced anti-metastatic bioactivity of an IGF-TRAP re-engineered to improve physicochemical properties
    Authors: G Vaniotis, S Moffett, T Sulea, N Wang, SM Elahi, E Lessard, J Baardsnes, S Perrino, Y Durocher, J Frystyk, B Massie, P Brodt
    Sci Rep, 2018-11-26;8(1):17361.
    Species: Human
    Sample Types: Cell Lysates
  2. Insulin-like growth factor 2 silencing restores taxol sensitivity in drug resistant ovarian cancer.
    Authors: Brouwer-Visser, Jurriaan, Lee, Jiyeon, McCullagh, KellyAnn, Cossio, Maria J, Wang, Yanhua, Huang, Gloria S
    PLoS ONE, 2014-06-16;9(6):e100165.
    Species: Human
    Sample Types: Cell Lysates
  3. Improvement of human keratinocyte migration by a redox active bioelectric dressing.
    Authors: Banerjee J, Das Ghatak P, Roy S, Khanna S, Sequin E, Bellman K, Dickinson B, Suri P, Subramaniam V, Chang C, Sen C
    PLoS ONE, 2014-03-03;9(3):e89239.
    Species: Human
    Sample Types: Cell Lysates
  4. Effect of hyperinsulinemia during hemodialysis on the insulin-like growth factor system and inflammatory biomarkers: a randomized open-label crossover study.
    Authors: Reinhard M, Frystyk J, Jespersen B, Bjerre M, Christiansen J, Flyvbjerg A, Ivarsen P
    BMC Nephrol, 2013-04-04;14(0):80.
    Species: Human
    Sample Types: Cell Lysates
  5. Activated phosphoinositide 3-kinase/AKT signaling confers resistance to trastuzumab but not lapatinib.
    Authors: O'Brien NA, Browne BC, Chow L, Wang Y, Ginther C, Arboleda J, Duffy MJ, Crown J, O'Donovan N, Slamon DJ
    Mol. Cancer Ther., 2010-05-25;9(6):1489-502.
    Species: Human
    Sample Types: Cell Lysates

FAQs

  1. Which phosphorylated sites are recognized by this assay?

    • This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

View all ELISA FAQs
Loading...

Reviews for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA

There are currently no reviews for this product. Be the first to review Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA and earn rewards!

Have you used Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review