Substrate Reagent Pack Summary
INTENDED USE & DESCRIPTION
Substrate Reagent Pack contains 8 vials of Color Reagent A (stabilized hydrogen peroxide, Part # 895000) and 8 vials of Color Reagent B (stabilized tetramethylbenzidine, Part # 895001), both at 12 mL/vial, producing color development when contacting HRP-conjugated or biotinylated, streptavidin-HRP bound detection antibodies.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.
REAGENT PREPARATION
Warm to room temperature before use. Color Reagent A and Color Reagent B should be mixed together in equal volumes within 15 minutes of use. Protect from light. Add 100 µL of the mixture to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994) and read the microplate.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
- Caution: Color Reagent B may cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.
Specifications
Product Datasheets
Citations for Substrate Reagent Pack
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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DNA-delivered monoclonal antibodies targeting the p53 R175H mutant epitope inhibit tumor development in mice
Authors: Chai, D;Wang, X;Neeli, P;Zhou, S;Yu, X;Sabapathy, K;Li, Y;
Genes & diseases 2024-07-01
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MANF regulates neuronal survival and UPR through its ER-located receptor IRE1alpha
Authors: V Kovaleva, LY Yu, L Ivanova, O Shpironok, J Nam, A Eesmaa, EP Kumpula, S Sakson, U Toots, M Ustav, JT Huiskonen, MH Voutilaine, P Lindholm, M Karelson, M Saarma
Cell Reports, 2023-02-03;42(2):112066. 2023-02-03
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Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors: S Choi, Z Yang, Q Wang, Z Qiao, M Sun, J Wiggins, SH Xiang, Q Lu
Science Advances, 2023-01-27;9(4):eade2708. 2023-01-27
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Does the Blood-Brain Barrier Integrity Change in Regard to the Onset of Fetal Growth Restriction?
Authors: N Misan, S Michalak, K Kapska, K Osztynowic, M Ropacka-Le, K Kawka-Paci
International Journal of Molecular Sciences, 2023-01-19;24(3):. 2023-01-19
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Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
Authors: MJ Rodrigo, I Bravo-Osun, M Subias, A Montolío, J Cegoñino, T Martinez-R, S Mendez-Mar, A Aragón-Nav, D Garcia-Her, LE Pablo, R Herrero-Va, AP Del Paloma, E Garcia-Mar
Scientific Reports, 2022-11-30;12(1):20622. 2022-11-30
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Dual Targeting of Soluble Oligomeric and Aggregated Transthyretin with a Monoclonal Antibody Ameliorates Experimental Neuropathy
Authors: M Fassler, S Tshori, Y Barac, DE Bowles, C Benaim, J George
Biology, 2022-10-15;11(10):. 2022-10-15
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A TGFbetaR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells
Authors: EW Fowler, EJ van Venroo, RL Witt, X Jia
Scientific Reports, 2022-09-02;12(1):15008. 2022-09-02
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A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGF
Authors: A Elgaabari, N Imatomi, H Kido, M Seki, S Tanaka, Y Matsuyoshi, T Nakashima, S Sawano, W Mizunoya, T Suzuki, M Nakamura, JE Anderson, R Tatsumi
Biochemistry and Biophysics Reports, 2022-06-11;31(0):101295. 2022-06-11
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Altered IgG glycosylation at COVID-19 diagnosis predicts disease severity
Authors: MM Vicente, I Alves, J Gaifem, CS Rodrigues, Â Fernandes, AM Dias, J Štambuk, T Petrovi?, P Oliveira, F Ferreira-d, A Soares, N Seixas, T Teixeira, L Malheiro, MM Abreu, G Lauc, R Sarmento E, SS Pinho
European Journal of Immunology, 2022-04-04;0(0):. 2022-04-04
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Novel efficacious microRNA-30c analogs reduce apolipoprotein B secretion in human hepatoma and primary hepatocyte cells
Authors: PK Yadav, P Haruehanro, S Irani, T Wang, A Ansari, J Sheng, MM Hussain
The Journal of Biological Chemistry, 2022-03-10;0(0):101813. 2022-03-10
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Cardioprotective effects of exercise preconditioning on ischemia-reperfusion injury and ventricular ectopy in young and senescent rats
Authors: A Fatahi, E Zarrinkala, K Azizbeigi, K Ranjbar
Experimental gerontology, 2022-03-02;162(0):111758. 2022-03-02
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E-Cigarette Aerosols Promote Oral S. aureus Colonization by Delaying an Immune Response and Bacterial Clearing
Authors: AR Cátala-Val, J Almeda, JN Bernard, AM Cole, AL Cole, SD Moore, CD Andl
Cells, 2022-02-23;11(5):. 2022-02-23
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Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive Model
Authors: KT Liu, YN Gong, CG Huang, PN Huang, KY Yu, HC Lee, SC Lee, HJ Chiang, YA Kung, YT Lin, MJ Hsiao, PW Huang, SY Huang, HT Wu, CC Wu, RL Kuo, KF Chen, CT Hung, KY Oguntuyo, CS Stevens, S Kowdle, HP Chiu, B Lee, GW Chen, SR Shih
mSphere, 2022-02-02;7(1):e0088321. 2022-02-02
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Development of a Low-Molecular-Weight Abeta42 Detection System Using a Enzyme-Linked Peptide Assay
Authors: SH Kim, EH Lee, HJ Kim, AR Kim, YE Kim, JH Lee, MY Yoon, SH Koh
Biomolecules, 2021-12-02;11(12):. 2021-12-02
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Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
Nature, 2021-11-03;0(0):. 2021-11-03
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Effect of PLC-beta1/CaM signaling pathway mediated by AT1R on the occurrence and development of hepatocellular carcinoma
Authors: ZW Xu, NN Liu, XY Wang, BC Ding, HF Zhang, Y Li, WY Sun, W Wei
Cancer Cell International, 2021-11-02;21(1):587. 2021-11-02
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Serum IL-18 Is a Potential Biomarker for Predicting Severe Dengue Disease Progression
Authors: JD Nanda, CJ Jung, RD Satria, MK Jhan, TJ Shen, PC Tseng, YT Wang, TS Ho, CF Lin
Journal of Immunology Research, 2021-10-25;2021(0):7652569. 2021-10-25
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A simple detection method for the serum sFLT1 protein in preeclampsia
Authors: M Shibuya, H Matsui, T Sasagawa, T Nagamatsu
Scientific Reports, 2021-10-18;11(1):20613. 2021-10-18
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Small circular interfering RNAs (sciRNAs) as a potent therapeutic platform for gene-silencing
Authors: H Jahns, R Degaonkar, P Podbevsek, S Gupta, A Bisbe, K Aluri, J Szeto, P Kumar, S LeBlanc, T Racie, CR Brown, A Castoreno, DC Guenther, V Jadhav, MA Maier, J Plavec, M Egli, M Manoharan, I Zlatev
Nucleic Acids Research, 2021-10-11;0(0):. 2021-10-11
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Identification of soluble tissue-derived biomarkers from human thyroid tissue explants maintained on a microfluidic device
Authors: A Riley, H Jones, J England, D Kuvshinov, V Green, J Greenman
Oncology Letters, 2021-09-13;22(5):780. 2021-09-13
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Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAs
Authors: J Konovalova, D Gerasymchu, SN Arroyo, S Kluske, F Mastroiann, AV Pereyra, A Domanskyi
International Journal of Molecular Sciences, 2021-09-07;22(18):. 2021-09-07
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(-)-Carveol Prevents Gastric Ulcers via Cytoprotective, Antioxidant, Antisecretory and Immunoregulatory Mechanisms in Animal Models
Authors: CAL Serafim, MEC Araruna, EB Alves Júni, LMO Silva, AO Silva, MS da Silva, AF Alves, AA Araújo, LM Batista
Frontiers in Pharmacology, 2021-08-23;12(0):736829. 2021-08-23
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LRG1 Expression Is Elevated in the Eyes of Patients with Neovascular Age-Related Macular Degeneration
Authors: L Mundo, GM Tosi, S Lazzi, G Pertile, B Parolini, G Neri, M Posarelli, E De Benedet, T Bacci, E Silvestri, MC Siciliano, S Barbera, M Orlandini, J Greenwood, SE Moss, F Galvagni
International Journal of Molecular Sciences, 2021-08-18;22(16):. 2021-08-18
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Acetylation of Calmodulin Regulates Synaptic Plasticity and Fear Learning
Authors: HL Zhang, B Zhao, W Han, YB Sun, P Yang, Y Chen, D Ni, J Zhang, DM Yin
The Journal of Biological Chemistry, 2021-07-31;0(0):101034. 2021-07-31
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Short-term treatment of golden retriever muscular dystrophy (GRMD) dogs with rAAVrh74.MHCK7.GALGT2 induces muscle glycosylation and utrophin expression but has no significant effect on muscle strength
Authors: PT Martin, DA Zygmunt, A Ashbrook, S Hamilton, D Packer, SM Birch, AK Bettis, CJ Balog-Alva, LJ Guo, PP Nghiem, JN Kornegay
PLoS ONE, 2021-03-26;16(3):e0248721. 2021-03-26
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&alpha-Phellandrene exhibits antinociceptive and tumor-reducing effects in a mouse model of oncologic pain
Authors: FR Pinheiro-N, EM Lopes, BT Acha, LDS Gomes, WA Dias, ACD Reis Filho, BS Leal, DCDN Rodrigues, JDN Silva, D Dittz, PMP Ferreira, FRC Almeida
Toxicology and Applied Pharmacology, 2021-03-17;418(0):115497. 2021-03-17
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Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex
Authors: R González-P, K Eifler-Oli, LA Claessens, E Willemstei, Z Xiao, CMP Talavera O, H Ovaa, HD Ulrich, ACO Vertegaal
Cell Reports, 2021-01-26;34(4):108691. 2021-01-26
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In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
Authors: T Kishimoto, W Ishida, T Nasukawa, T Ujihara, I Nakajima, T Suzuki, J Uchiyama, D Todokoro, M Daibata, A Fukushima, S Matsuzaki, K Fukuda
Microorganisms, 2021-01-20;9(2):. 2021-01-20
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Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein
Authors: CD Hodge, DJ Rosenberg, M Wilamowski, A Joachimiak, GL Hura, M Hammel
bioRxiv : the preprint server for biology, 2021-01-13;0(0):. 2021-01-13
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Double stranded RNA drives innate immune responses, sickness behavior and cognitive impairment dependent on dsRNA length, IFNAR1 expression and age
Authors: N McGarry, C Murray, S Garvey, A Wilkinson, L Tortorelli, L Ryan, L Hayden, D Healy, EW Griffin, E Hennessy, DT Skelly, M Arumugam, KJ Mitchell, C Cunningham
bioRxiv : the preprint server for biology, 2021-01-09;0(0):. 2021-01-09
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Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
Authors: L Kang, S Smith, C Wang
Journal of colloid and interface science, 2020-12-24;588(0):101-109. 2020-12-24
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Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana
Authors: K Rattanapis, B Shanmugara, S Manopwised, PB Purwono, K Siriwattan, N Khorattana, O Hanittinan, W Boonyayoth, A Thitithany, DR Smith, W Phoolcharo
Sci Rep, 2020-10-19;10(1):17698. 2020-10-19
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The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response
Authors: S Lee, S Kim, HY Kang, HR Lim, Y Kwon, M Jo, YM Jeon, SR Kim, K Kim, CM Ha, S Lee, HJ Kim
J Neuroinflammation, 2020-10-14;17(1):299. 2020-10-14
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TRI microparticles prevent inflammatory arthritis in a collagen-induced arthritis model
Authors: EJ Bassin, AR Buckley, JD Piganelli, SR Little
PLoS ONE, 2020-09-23;15(9):e0239396. 2020-09-23
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SARS-CoV-2 Spike protein co-opts VEGF-A/Neuropilin-1 receptor signaling to induce analgesia
Authors: A Moutal, LF Martin, L Boinon, K Gomez, D Ran, Y Zhou, HJ Stratton, S Cai, S Luo, KB Gonzalez, S Perez-Mill, A Patwardhan, MM Ibrahim, R Khanna
bioRxiv : the preprint server for biology, 2020-09-14;0(0):. 2020-09-14
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Cross-reactive antibody response between SARS-CoV-2 and SARS-CoV infections
Authors: H Lv, NC Wu, OT Tsang, M Yuan, RAPM Perera, WS Leung, RTY So, JMC Chan, GK Yip, TSH Chik, Y Wang, CYC Choi, Y Lin, WW Ng, J Zhao, LLM Poon, JSM Peiris, IA Wilson, CKP Mok
Cell Rep, 2020-05-18;31(9):107725. 2020-05-18
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Cross-reactive Antibody Response between SARS-CoV-2 and SARS-CoV Infections
Authors: H Lv, NC Wu, OT Tsang, M Yuan, RAPM Perera, WS Leung, RTY So, JMC Chan, GK Yip, TSH Chik, Y Wang, CYC Choi, Y Lin, WW Ng, J Zhao, LLM Poon, JSM Peiris, IA Wilson, CKP Mok
Cell Rep, 2020-01-01;31(9):107725. 2020-01-01
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Utility of Centrifugation-Controlled Convective (C3) Flow for Rapid On-chip ELISA
Authors: W Espulgar, T Tadokoro, E Tamiya, M Saito
Sci Rep, 2019-12-27;9(1):20150. 2019-12-27
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Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
Int J Nanomedicine, 2019-08-01;14(0):6117-6131. 2019-08-01
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Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats
Authors: M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita
J. Neurosci., 2019-07-25;0(0):. 2019-07-25
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Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
J Clin Med, 2019-07-15;8(7):. 2019-07-15
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Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
Cell Rep, 2019-06-11;27(11):3117-3123.e5. 2019-06-11
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Infliximab Induced a Dissociated Response of Severe Periodontal Biomarkers in Rheumatoid Arthritis Patients
Authors: M Rinaudo-Ga, V Blasco-Baq, P Miossec, P Gaudin, P Farge, X Roblin, T Thomas, S Paul, H Marotte
J Clin Med, 2019-05-26;8(5):. 2019-05-26
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Nkx-2.5 Regulates MDR1 Expression via Its Upstream Promoter in Breast Cancer Cells
Authors: JS Lim, GY Jung, SY Park
J. Korean Med. Sci., 2019-03-19;34(12):e100. 2019-03-19
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IL12 p35 and p40 subunit genes administered as pPAL plasmid constructs do not improve protection of pPAL-LACK vaccine against canine leishmaniasis
Authors: PJ Alcolea, A Alonso, A Esteban, P Peris, A Cortés, JA Castillo, V Larraga
PLoS ONE, 2019-02-22;14(2):e0212136. 2019-02-22
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GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites
Authors: N Morita, E Umemoto, S Fujita, A Hayashi, J Kikuta, I Kimura, T Haneda, T Imai, A Inoue, H Mimuro, Y Maeda, H Kayama, R Okumura, J Aoki, N Okada, T Kida, M Ishii, R Nabeshima, K Takeda
Nature, 2019-01-23;0(0):. 2019-01-23
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Natural Killer Response and Lipo-Metabolic Profile in Adults with Low HDL-Cholesterol and Mild Hypercholesterolemia: Beneficial Effects of Artichoke Leaf Extract Supplementation
Authors: M Rondanelli, AM Castellazz, A Riva, P Allegrini, MA Faliva, G Peroni, M Naso, M Nichetti, C Tagliacarn, C Valsecchi, T Fazia, S Perna, F Graziano, M Grassi, L Bernardine
Evid Based Complement Alternat Med, 2019-01-06;2019(0):2069701. 2019-01-06
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SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4
Authors: K Tzelepis, E De Braekel, D Aspris, I Barbieri, MS Vijayabask, WH Liu, M Gozdecka, E Metzakopia, HD Toop, M Dudek, SC Robson, F Hermida-Pr, YH Yang, R Babaei-Jad, DA Garyfallos, H Ponstingl, JML Dias, P Gallipoli, M Seiler, S Buonamici, B Vick, AJ Bannister, R Rad, RK Prinjha, JC Marioni, B Huntly, J Batson, JC Morris, C Pina, A Bradley, I Jeremias, DO Bates, K Yusa, T Kouzarides, GS Vassiliou
Nat Commun, 2018-12-19;9(1):5378. 2018-12-19
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Elevated Hsp90-beta contributes to differential diagnosis of pleural effusion caused by lung cancer and correlates with malignant biological behavior of lung cancer
Authors: R Biaoxue, L Min, F Tian, G Wenlong, L Hua
BMC Pulm Med, 2018-12-06;18(1):188. 2018-12-06
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Safety Studies of Pneumococcal Endolysins Cpl-1 and Pal
Authors: M Harhala, DC Nelson, P Miernikiew, RD Heselpoth, B Brzezicka, J Majewska, SB Linden, X Shang, A Szymczak, D Lecion, K Marek-Buko, M K?ak, B Wojciechow, K Lahutta, A Konieczny, K D?browska
Viruses, 2018-11-15;10(11):. 2018-11-15
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VSIG-3 as a ligand of VISTA inhibits human T-cell function.
Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
Immunology, 2018-10-10;156(1):74-85. 2018-10-10
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GM1 ganglioside prevents axonal regeneration inhibition and cognitive deficits in a mouse model of traumatic brain injury
Authors: A Benady, D Freidin, CG Pick, V Rubovitch
Sci Rep, 2018-09-06;8(1):13340. 2018-09-06
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H7 Hemagglutinin nanoparticles retain immunogenicity after >3 months of 25�C storage
Authors: TZ Chang, L Deng, BZ Wang, JA Champion
PLoS ONE, 2018-08-09;13(8):e0202300. 2018-08-09
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Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
Sci Rep, 2018-07-09;8(1):10328. 2018-07-09
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Inhibition of anthrax lethal factor by ssDNA aptamers
Authors: M Lahousse, HC Park, SC Lee, NR Ha, IP Jung, SR Schlesinge, K Shackelfor, MY Yoon, SK Kim
Arch. Biochem. Biophys., 2018-03-24;646(0):16-23. 2018-03-24
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Soluble CD14 as a Diagnostic Biomarker for Smear-Negative HIV-Associated Tuberculosis
Authors: Y Liu, OC Ndumnego, T Chen, RS Kim, ER Jenny-Avit, T Ndung'u, D Wilson, JM Achkar
Pathogens, 2018-02-27;7(1):. 2018-02-27
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Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
Authors: J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney
Adv Healthc Mater, 2018-02-14;0(0):. 2018-02-14
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Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
Authors: M Oleszowsky, MF Seidel
Biomed Res Int, 2018-02-01;2018(0):8286067. 2018-02-01
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Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
Planta, 2018-01-08;0(0):. 2018-01-08
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Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis
Authors: H Lin, R Muramatsu, N Maedera, H Tsunematsu, M Hamaguchi, Y Koyama, M Kuroda, K Ono, M Sawada, T Yamashita
EBioMedicine, 2017-12-07;0(0):. 2017-12-07
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The ESRP1-GPR137 axis contributes to intestinal pathogenesis
Authors: LF Mager, VH Koelzer, R Stuber, L Thoo, I Keller, I Koeck, M Langenegge, C Simillion, SP Pfister, M Faderl, V Genitsch, I Tcymbarevi, P Juillerat, X Li, Y Xia, E Karamitopo, R Lyck, I Zlobec, S Hapfelmeie, R Bruggmann, KD McCoy, AJ Macpherson, C Müller, B Beutler, P Krebs
Elife, 2017-10-04;6(0):. 2017-10-04
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YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation
Authors: M Martinez-G, KGK Goh, E Rackaityte, C Sakamoto, B Audrain, DG Moriel, M Totsika, JM Ghigo, MA Schembri, C Beloin
Sci Rep, 2017-09-12;7(1):11326. 2017-09-12
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Vitronectin as a Micromanager of Cell Response in Material-Driven Fibronectin Nanonetworks
Authors: M Cantini, K Gomide, V Moulisova, C González-G, M Salmerón-S
Adv Biosyst, 2017-08-10;1(9):1700047. 2017-08-10
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Lettuce-produced hepatitis C virus E1E2 heterodimer triggers immune responses in mice and antibody production after oral vaccination
Authors: J Liu Clarke, L Paruch, MO Dobrica, I Caras, C Tucureanu, A Onu, S Ciulean, C Stavaru, A Eerde, Y Wang, H Steen, S Haugslien, C Petrareanu, C Lazar, I Popescu, R Bock, J Dubuisson, N Branza-Nic
Plant Biotechnol. J., 2017-06-09;0(0):. 2017-06-09
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IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
JCI Insight, 2017-05-18;2(10):. 2017-05-18
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Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate
Authors: RL Tallmadge, SC Miller, SA Parry, MJB Felippe
PLoS ONE, 2017-05-16;12(5):e0177831. 2017-05-16
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Demyelination induced by oxidative stress is regulated by sphingosine 1-phosphate receptors
Authors: SA O'Sullivan, M Velasco-Es, KK Dev
Glia, 2017-04-04;0(0):. 2017-04-04
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Evaluation of multivalent H2 influenza pandemic vaccines in mice
Authors: BJ Lenny, S Sonnberg, AF Danner, K Friedman, RJ Webby, RG Webster, JC Jones
Vaccine, 2017-02-08;0(0):. 2017-02-08
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Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury
Authors: V Rubovitch, Y Zilberstei, J Chapman, S Schreiber, CG Pick
Sci Rep, 2017-01-23;7(0):41269. 2017-01-23
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Neuroprotection by Caffeine in Hyperoxia-Induced Neonatal Brain Injury
Authors: S Endesfelde, U Weichelt, E Strauá, A Schl”r, M Sifringer, T Scheuer, C Bhrer, T Schmitz
Int J Mol Sci, 2017-01-18;18(1):. 2017-01-18
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The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization
Nat Commun, 2016-11-24;7(0):13517. 2016-11-24
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Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina
PLoS ONE, 2016-08-26;11(8):e0161898. 2016-08-26
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Marked elevation in plasma osteoprotegerin constitutes an early and consistent feature of cerebral malaria
Authors: James S O'Donnell
Thromb. Haemost., 2016-01-14;115(4):773-80. 2016-01-14
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Non-Human Primates Harbor Diverse Mammalian and Avian Astroviruses Including Those Associated with Human Infections.
Authors: Karlsson E, Small C, Freiden P, Feeroz M, Matsen F, San S, Hasan M, Wang D, Jones-Engel L, Schultz-Cherry S
PLoS Pathog, 2015-11-16;11(11):e1005225. 2015-11-16
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Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation.
Authors: Kim B, Rongisch R, Hager S, Grieb G, Nourbakhsh M, Rennekampff H, Bucala R, Bernhagen J, Pallua N
PLoS ONE, 2015-09-08;10(9):e0137366. 2015-09-08
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Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
J Neuroinflammation, 2015-06-16;12(0):119. 2015-06-16
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Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts.
Authors: Lee W, Shen S, Shih Y, Chou C, Tseng J, Chin S, Liu K, Chen Y, Jiang M
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Mechanism of the antigen-independent cytokinergic SPE-7 IgE activation of human mast cells in vitro.
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Innate immunity drives the initiation of a murine model of primary biliary cirrhosis.
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An automated method for determining the cytoadhesion of Plasmodium falciparum-infected erythrocytes to immobilized cells.
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A C-terminal HSP90 inhibitor restores glucocorticoid sensitivity and relieves a mouse allograft model of Cushing disease.
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Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
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Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling.
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Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.
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Upregulated interleukin-21 receptor on B cells associated with the downregulation of IgE in patients with allergic rhinitis.
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Hepatocytes induce Foxp3(+) regulatory T cells by Notch signaling.
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J Leukoc Biol, 2014-06-26;96(4):571-7. 2014-06-26
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Dimerization is not a determining factor for functional high affinity human plasminogen binding by the group A streptococcal virulence factor PAM and is mediated by specific residues within the PAM a1a2 domain.
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Netrin-1 controls sympathetic arterial innervation.
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Detection of antibodies against Turkey astrovirus in humans.
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PLoS ONE, 2014-05-14;9(5):e96934. 2014-05-14
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A soluble form of IL-27Ralpha is a natural IL-27 antagonist.
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J Immunol, 2014-04-25;192(11):5382-9. 2014-04-25
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The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
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GPR56 inhibits melanoma growth by internalizing and degrading its ligand TG2.
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Shedding of c-Met ectodomain correlates with c-Met expression in non-small cell lung cancer.
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Latent TGF-beta binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5.
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Adiponectin inhibits leptin signalling via multiple mechanisms to exert protective effects against hepatic fibrosis.
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Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
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J. Immunol., 2011-10-21;187(11):5745-55. 2011-10-21
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Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
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Apolipoprotein B binding domains: evidence that they are cell-penetrating peptides that efficiently deliver antigenic peptide for cross-presentation of cytotoxic T cells.
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Decompensated heart failure is associated with reduced corin levels and decreased cleavage of pro-atrial natriuretic peptide.
Authors: Ibebuogu UN, Gladysheva IP, Houng AK
Circ Heart Fail, 2011-01-07;4(2):114-20. 2011-01-07
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Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
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PLoS ONE, 2010-10-21;5(10):e13568. 2010-10-21
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PEGylation of interleukin-10 for the mitigation of enhanced pain states.
Authors: Soderquist RG, Milligan ED, Harrison JA, Chavez RA, Johnson KW, Watkins LR, Mahoney MJ
J Biomed Mater Res A, 2010-06-01;93(3):1169-79. 2010-06-01
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Enzyme-linked enzyme-binding assay for Pin1 WW domain ligands.
Authors: Mercedes-Camacho AY, Etzkorn FA
Anal. Biochem., 2010-03-15;402(1):77-82. 2010-03-15
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Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.
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Vaccine, 2010-02-23;28(8):1942-51. 2010-02-23
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A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa.
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Mucosal Immunol, 2009-10-07;3(1):40-8. 2009-10-07
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Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
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Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta.
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Am. J. Pathol., 2009-06-18;175(1):262-70. 2009-06-18
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A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
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Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
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Clin. Exp. Immunol., 2008-03-18;152(2):328-35. 2008-03-18
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Nanobacteria are mineralo fetuin complexes.
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Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
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Chest, 2007-05-15;132(1):58-63. 2007-05-15
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Differential gene expression in cultured osteoblasts and bone marrow stromal cells from patients with Paget's disease of bone.
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The effects of dehydroepiandrosterone (DHEA) on in vitro spleen cell proliferation and cytokine production.
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J. Interferon Cytokine Res., 2006-01-01;26(1):34-9. 2006-01-01
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Human cytomegalovirus-specific CD4+-T-cell cytokine response induces fractalkine in endothelial cells.
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J. Virol., 2004-12-01;78(23):13173-81. 2004-12-01
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Selective roles for tumor necrosis factor alpha-converting enzyme/ADAM17 in the shedding of the epidermal growth factor receptor ligand family: the juxtamembrane stalk determines cleavage efficiency.
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ELR+ CXC chemokines in human milk.
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Cytokine, 2003-11-07;24(3):91-102. 2003-11-07
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Substrate works well in direct and sandwich ELISAs
One pair of A and B is enough for one plate which makes it very easy to use while keep the reagent stable
I used this a my detection substrate (100uL) in a direct ELISA after my plate was treated with an anti-mouse IgG-HRP antibody. I find the background is very low which allows me to achieved a better sensitivity with my ELISAs compared another commercial substrate
It is very stable before mixing the reagent A and B. One bottle of each is exactly enough for two plates which makes measuring simple.
Our lab does many ELISAs. These substrate solutions are excellent, affordable, and easy to use.
Really Easy to use.
After the SA-HRP detection step for ELISA, wash wells 4 times with wash buffer, and add the Substrate and incubate for 20 mins in the dark. Stop the reaction using 0.2 M sulfuric acid. Read the plate at 450nm