Human/Primate Chitinase 3-like 1/YKL-40 Biotinylated Antibody

Name: Human/Primate Chitinase 3-like 1/YKL-40 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF2599
Rating: 5 (1 reviews)

Catalog #: BAF2599
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF2599

All Chitinase 3-like 1/YKL-40 Antibodies

Product Details

Citations (1)

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Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate Chitinase 3-like 1/YKL-40 Biotinylated Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human and primate Chitinase 3-like 1 in ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Chitinase 3-like 1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 1 (R&D Systems, Catalog # 2599-CH)
Tyr22-Thr383
Accession # P36222

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Chitinase 3-like 1 (Catalog # 2599-CH)

Human/Primate Chitinase 3-like 1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human/Primate Chitinase 3‑like 1 Antibody (Catalog # MAB25991)

Standard: Recombinant Human Chitinase 3-like 1 Protein, CF (Catalog # 2599-CH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5‑7). The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides (3). Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1‑4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15).

References

Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
Shao, R. et al. (2009) Oncogene 28:4456.
Recklies, A.D. et al. (2002) Biochem. J. 365:119.
Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
Letuve, S. et al. (2008) J Immunol. 181:5167.
Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
Johansen, J.S. et al. (1999) Rheumatology 38:618.
Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.

Entrez Gene IDs

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Alternate Names

AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Primate Chitinase 3-like 1/YKL-40 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Low Plasma IL-8 Levels During Chemotherapy Are Predictive of Excellent Long-Term Survival in Metastatic Breast Cancer
Authors: L Tiainen, M Hämäläinen, T Luukkaala, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, E Moilanen, PL Kellokumpu
Clin. Breast Cancer, 2019-04-04;0(0):.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection