Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF
Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF Summary
Product Specifications
Tyr22-Thr383, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2599-CH
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Chitinase 3-like 1/YKL-40
Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides (3). Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15).
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- Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
- Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
- Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
- Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
- Shao, R. et al. (2009) Oncogene 28:4456.
- Recklies, A.D. et al. (2002) Biochem. J. 365:119.
- Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
- Letuve, S. et al. (2008) J Immunol. 181:5167.
- Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
- Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
- Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
- Johansen, J.S. et al. (1999) Rheumatology 38:618.
- Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.
Citations for Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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CHI3L1 signaling impairs hippocampal neurogenesis and cognitive function in autoimmune-mediated neuroinflammation
Authors: Jiang, W;Zhu, F;Xu, H;Xu, L;Li, H;Yang, X;Khan Afridi, S;Lai, S;Qiu, X;Liu, C;Li, H;Long, Y;Wang, Y;Connolly, K;Elias, JA;Lee, CG;Cui, Y;Huang, YA;Qiu, W;Tang, C;
Science advances
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation
Authors: SC Starossom, J Campo Garc, T Woelfle, S Romero-Sua, M Olah, F Watanabe, L Cao, A Yeste, JJ Tukker, FJ Quintana, J Imitola, F Witzel, D Schmitz, M Morkel, F Paul, C Infante-Du, SJ Khoury
Nat Commun, 2019-01-15;10(1):217.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress.
Authors: Park JA, Drazen JM, Tschumperlin DJ
J. Biol. Chem., 2010-07-22;285(39):29817-25.
Applications: Western Blot
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The recombinant Chitinase 3-like 1 (YKL-40) worked well as a standard in an ELISA made to measure YKL-40 in human serum samples.
We use this item as a positive control for IP experiments.
