Human/Primate EGF Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF236
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human/Primate EGF Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human EGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross-reactivity with rmEGF, rhEGF R and rhHB-EGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EGF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human EGF (Catalog # 236-EG)
under non-reducing conditions only

Human/Primate EGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate EGF Antibody (Catalog # MAB636)

Standard: Recombinant Human EGF Protein, CF (Catalog # 236-EG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin-binding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF-like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

References
  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.
Long Name
Epidermal Growth Factor
Entrez Gene IDs
1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone

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Citations for Human/Primate EGF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Development and Validation of a Good Manufacturing Process for IL-4-Driven Expansion of Chimeric Cytokine Receptor-Expressing CAR T-Cells
    Authors: MCI van Schalk, SJC van der St, L Bosshard-C, H Graves, S Papa, AC Parente-Pe, F Farzaneh, CD Fisher, A Hope, A Adami, J Maher
    Cells, 2021-07-15;10(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry (CAR-T)
  2. Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light.
    Authors: Wirth S, Segretin ME, Mentaberry A, Bravo-Almonacid F
    J. Biotechnol., 2006-04-03;125(2):159-72.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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Reviews for Human/Primate EGF Biotinylated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human/Primate EGF Biotinylated Antibody
By Alisha Freeman on 04/05/2018
Application: Cell Depl Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse

Human/Primate EGF Biotinylated Antibody
By Alisha Freeman on 10/27/2017
Application: Affinity Purification Sample Tested: 293-EBNA human EBV-expressing embryonic kidney cell line Species: Human