Human TCF-2/HNF-1 beta Antibody

Catalog #: AF3330 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3330
AF3330-SP

TCF-2/HNF-1 beta in BG01V Human Embryonic Stem Cells.

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Human TCF-2/HNF-1 beta Antibody Summary

Species Reactivity

Human

Specificity

This antibody detects TCF‑2/HNF‑1 beta in direct ELISAs and Western blots. It is expected to recognize all three TCF‑2 isoforms.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TCF‑2/HNF‑1 beta
Gly51-Gln182
Accession # P35680

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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TCF-2/HNF-1 beta in BG01V Human Embryonic Stem Cells. TCF-2/HNF-1 beta was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry

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TCF‑2/HNF‑1 beta in Human Kidney. TCF-2/HNF-1 beta was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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TCF‑2/HNF‑1 beta in Rat Kidney. TCF-2/HNF-1 beta was detected in perfusion fixed frozen sections of rat kidney using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-2/HNF-1 beta

TCF-2, also known as HNF-1 beta (hepatocyte nuclear factor 1 beta ) and VHNF-1, is a member of the HNF-1 homeobox family. By alternative splicing, three isoforms of TCF-2/HNF-1 beta exist. TCF-2/HNF-1 beta binds DNA as a homodimer or heterodimer with TCF-1/HNF-1 alpha. Within the region used as the immunogen, human TCF-2 shares 98% amino acid sequence homology with mouse and rat TCF-2. TCF-2 expression is required for visceral endoderm specification.

Long Name

Transcription Factor 2

Entrez Gene IDs

6928 (Human); 21410 (Mouse); 25640 (Rat)

Alternate Names

FJHN; hepatocyte nuclear factor 1-beta; HNF1 beta A; HNF-1 beta; HNF1 homeobox B; HNF1B; HNF-1B; HNF1beta; HNF-1-beta; HNF2; Homeoprotein LFB3; LFB3; LF-B3; MODY5; MODY5HPC11; TCF2; TCF-2; TCF2transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor; Transcription factor 2; transcription factor 2, hepatic; Variant hepatic nuclear factor 1; VHNF1