Human Thrombopoietin/Tpo Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM288
Product Details
Citations (1)
FAQs
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Human Thrombopoietin/Tpo Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tpo in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, or rhM-CSF.
Source
Monoclonal Mouse IgG2A Clone # 34840
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tpo
Ser22-Gly353
Accession # P40225
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Protein A or G purified from ascites

Applications

Recommended Concentration
Sample

Human Thrombopoietin/Tpo Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human Thrombopoietin/Tpo Antibody (Catalog # MAB288)

Detection Reagent: Human Thrombopoietin/Tpo Biotinylated Antibody (Catalog # BAF288)

Standard: Recombinant Human Thrombopoietin (NS0-expressed) Protein (Catalog # 288-TPN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin/Tpo

Tpo is a secreted protein that regulates megakaryocytopoiesis and thrombopoiesis. It binds to and induces signaling through the thrombopoietin receptor.

Entrez Gene IDs
7066 (Human); 21832 (Mouse); 81811 (Rat)
Alternate Names
Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

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Citation for Human Thrombopoietin/Tpo Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development

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