Home / TIMP Assay Kits / Human TIMP Luminex® Performance Assay 3-plex Fixed Panel

Human TIMP Luminex® Performance Assay 3-plex Fixed Panel

Catalog #: LKTM003
12 Citations Datasheet / COA / SDS
For the measurement of TIMP-1, TIMP-2, and TIMP-4. Earn double rewards for reviews
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LKTM003

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Human TIMP Luminex Panel Standard Curves
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Citations (12)
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Summary Product Features Precision & Accuracy Data Examples Product Datasheets Background

Human TIMP Luminex® Performance Assay 3-plex Fixed Panel Summary

Assay Type bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and antibody-coated beads
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

  • Simultaneously profile up to 50 analytes of your choice in one sample
  • Choose from a wide menu of analytes, including many unique analytes
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-4 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate

Please allow additional time for international orders.

Kit Components
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Standard/Sample Diluent
  • Wash Buffer
  • Streptavidin-PE
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Certificate of Analysis**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay

Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 30 different serum assays.
  n=40 (20x2 levels) n=30
Analyte Intra-Assay (%CV) Inter-Assay (%CV)
TIMP-1 7.0 11.2
TIMP-2 4.3 9.9
TIMP-4 5.8 10.8

Accuracy

Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk.

Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
  Linearity Spiked
Recovery
Analyte Average % Average %
TIMP-1 111 NA
TIMP-2 100 84
TIMP-4 88 94
NA = Not applicable
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Scientific Data

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Human TIMP Luminex Panel Standard Curves

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Human TIMP Performance Luminex Panel Serum Sample Values Serum samples from 24 apparently healthy individuals were run on the Human TIMP Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

Background: TIMP Assay Kits

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP Assay Kits

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Human TIMP Luminex® Performance Assay 3-plex Fixed Panel

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Expression of Tissue Inhibitors of Metalloproteinases (TIMP-1, TIMP-2, TIMP-3, TIMP-4) in Blood Serum of Patients with Keratoconus
    Authors: Nowak-W?s, M;W?s, P;Czuba, Z;Wojnicz, R;Wygl?dowska-Promie?ska, D;
    Journal of clinical medicine
    Species: Human
    Sample Types: Serum
  2. Anethole induces anti-oral cancer activity by triggering apoptosis, autophagy and oxidative stress and by modulation of multiple signaling pathways
    Authors: C Contant, M Rouabhia, L Loubaki, F Chandad, A Semlali
    Scientific Reports, 2021-06-22;11(1):13087.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Markers of tissue remodeling and inflammation in the white and brown adipose tissues of a model hibernator
    Authors: SM Logan, KB Storey
    Cellular Signalling, 2021-03-09;82(0):109975.
    Species: Squirrel
    Sample Types: Tissue Homogenates
  4. Plasma levels of C-reactive protein, matrix metalloproteinase-7 and lipopolysaccharide-binding protein distinguish active pulmonary or extrapulmonary tuberculosis from uninfected controls in children
    Authors: VVS Albuquerqu, NP Kumar, KF Fukutani, B Vasconcelo, MB Arriaga, PS Silveira-M, S Babu, BB Andrade
    Cytokine, 2019-07-09;123(0):154773.
    Species: Human
    Sample Types: Plasma
  5. Adipose-derived stromal cell secretome reduces TNF?-induced hypertrophy and catabolic markers in primary human articular chondrocytes
    Authors: S Niada, C Giannasi, M Gomarasca, D Stanco, S Casati, AT Brini
    Stem Cell Res, 2019-05-15;38(0):101463.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Effects of aqueous suppressants and prostaglandin analogues on early wound healing after glaucoma implant surgery
    Authors: KI Jung, JE Woo, CK Park
    Sci Rep, 2019-03-27;9(1):5251.
    Species: Rabbit
    Sample Types: Aqueous Humor
  7. Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction
    Authors: N Akbar, JE Digby, TJ Cahill, AN Tavare, AL Corbin, S Saluja, S Dawkins, L Edgar, N Rawlings, K Ziberna, E McNeill,, E Johnson, AA Aljabali, RA Dragovic, M Rohling, TG Belgard, IA Udalova, DR Greaves, KM Channon, PR Riley, DC Anthony, RP Choudhury
    JCI Insight, 2017;2(17):.
    Species: Human
    Sample Types: Plasma
  8. Increased Circulating Endothelial Microparticles Associated with PAK4 Play a Key Role in Ventilation-Induced Lung Injury Process
    Authors: S Pan, A Fei, L Jing, X Zhang, C Gao
    Biomed Res Int, 2017;2017(0):4902084.
    Species: Mouse
    Sample Types: Plasma
  9. Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis.
    Authors: Andrade B, Pavan Kumar N, Amaral E, Riteau N, Mayer-Barber K, Tosh K, Maier N, Conceicao E, Kubler A, Sridhar R, Banurekha V, Jawahar M, Barbosa T, Manganiello V, Moss J, Fontana J, Marciano B, Sampaio E, Olivier K, Holland S, Jackson S, Moayeri M, Leppla S, Sereti I, Barber D, Nutman T, Babu S, Sher A
    J Immunol, 2015-08-12;195(6):2763-73.
    Species: Human
    Sample Types: Plasma
  10. Overexpression of MMP-9 and its inhibitors in blood mononuclear cells after myocardial infarction--is it associated with depressive symptomatology?
    Authors: Jonsson S, Lundberg A, Jonasson L
    PLoS ONE, 2014-08-25;9(8):e105572.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. A multi-analyte serum test for the detection of non-small cell lung cancer.
    Authors: Farlow EC, Vercillo MS, Coon JS, Basu S, Kim AW, Faber LP, Warren WH, Bonomi P, Liptay MJ, Borgia JA
    Br. J. Cancer, 2010;103(8):1221-8.
    Species: Human
    Sample Types: Serum
  12. Effects of weight loss from a very-low-carbohydrate diet on endothelial function and markers of cardiovascular disease risk in subjects with abdominal obesity.
    Authors: Keogh JB, Brinkworth GD, Noakes M, Belobrajdic DP, Buckley JD, Clifton PM
    Am. J. Clin. Nutr., 2008;87(3):567-76.
    Species: Human
    Sample Types: Serum

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