Human Tryptophan Hydroxylase 1/TPH-1 Antibody

Catalog #: MAB5276 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5276
MAB5276-SP

All Tryptophan Hydroxylase 1/TPH-1 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tryptophan Hydroxylase 1/TPH-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tryptophan Hydroxylase 1/TPH‑1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 508417

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tryptophan Hydroxylase 1/TPH‑1
Met102-Pro403
Accession # P17752

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Tryptophan Hydroxylase 1/TPH‑1

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Tryptophan Hydroxylase 1/TPH‑1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptophan Hydroxylase 1/TPH-1

Tryptophan Hydroxylase-1 (TPH-1) is a member of the pterin-dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The amino acid sequence of human TPH-1 is 96%, 90%, and 87% identical to that of cow, mouse, and chicken.

Entrez Gene IDs

7166 (Human); 21990 (Mouse); 24848 (Rat)

Alternate Names

EC 1.14.16; EC 1.14.16.4; indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; TPH-1; TPRH; TRPH; tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); Tryptophan Hydroxylase 1