Human uPAR Biotinylated Antibody

Catalog #: BAF807
4 Citations Datasheet / COA / SDS

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BAF807

All uPAR Antibodies

Product Details

Citations (4)

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Human uPAR Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human uPAR in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse uPAR and recombinant human uPA-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human uPAR (R&D Systems, Catalog # 807-UK)
Leu23-Arg303
Accession # Q03405

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human uPAR (Catalog # 807-UK)

Human uPAR Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human uPAR Antibody (Catalog # MAB807)

Standard: Recombinant Human uPAR Protein (Catalog # 807-UK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

References

Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.

Long Name

Urokinase-type Plasminogen Activator Receptor

Entrez Gene IDs

5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)

Alternate Names

CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

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Related Research Areas

Citations for Human uPAR Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels.
Authors: Portelli M, Siedlinski M, Stewart C, Postma D, Nieuwenhuis M, Vonk J, Nurnberg P, Altmuller J, Moffatt M, Wardlaw A, Parker S, Connolly M, Koppelman G, Sayers I
FASEB J, 2013-11-18;28(2):923-34.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development
Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB
Am. J. Respir. Cell Mol. Biol., 2006-05-25;35(4):503-11.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot