Recombinant Human uPAR Protein Summary
Product Specifications
Leu23-Arg303, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
807-UK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
807-UK/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: uPAR
The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a
single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a
C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.
Citations for Recombinant Human uPAR Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Simultaneous stabilization of actin cytoskeleton in multiple nephron-specific cells protects the kidney from diverse injury
Authors: K Mukherjee, C Gu, A Collins, M Mettlen, B Samelko, MM Altintas, YR Sudhini, X Wang, R Bouley, D Brown, BP Pedro, SL Bane, V Gupta, PT Brinkkoett, H Hagmann, J Reiser, S Sever
Nature Communications, 2022-05-03;13(1):2422.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Deiodinase-3 is a thyrostat to regulate podocyte homeostasis
Authors: S Agarwal, KH Koh, NJ Tardi, C Chen, RR Dande, JP WerneckdeC, YR Sudhini, C Luongo, D Salvatore, B Samelko, MM Altintas, S Mangos, A Bianco, J Reiser
EBioMedicine, 2021-10-11;72(0):103617.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Urinary myo-inositol is associated with the clinical outcome in focal segmental glomerulosclerosis
Authors: JN An, JS Hyeon, Y Jung, YW Choi, JH Kim, SH Yang, S Oh, S Kwon, SH Lee, JH Cho, SH Park, H Ha, DK Kim, JP Lee, GS Hwang
Sci Rep, 2019-10-11;9(1):14707.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
uPAR isoform 2 forms a dimer and induces severe kidney disease in mice
Authors: C Wei, J Li, BD Adair, K Zhu, J Cai, M Merchant, B Samelko, Z Liao, KH Koh, NJ Tardi, RR Dande, S Liu, J Ma, S DiBartolo, S Hägele, V Peev, SS Hayek, DJ Cimbaluk, M Tracy, JB Klein, S Sever, SJ Shattil, MA Arnaout, J Reiser
J. Clin. Invest., 2019-04-02;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: SDS-Page -
Urinary soluble urokinase receptor levels are elevated and pathogenic in patients with primary focal segmental glomerulosclerosis.
Authors: Huang J, Liu G, Zhang Y, Cui Z, Wang F, Liu X, Chu R, Zhao M
BMC Med, 2014-05-20;12(0):81.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Involvement of nitric oxide synthase in matrix metalloproteinase-9- and/or urokinase plasminogen activator receptor-mediated glioma cell migration.
Authors: Zhuang T, Chelluboina B, Ponnala S, Velpula K, Rehman A, Chetty C, Zakharian E, Rao J, Veeravalli K
BMC Cancer, 2013-12-11;13(0):590.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels.
Authors: Portelli M, Siedlinski M, Stewart C, Postma D, Nieuwenhuis M, Vonk J, Nurnberg P, Altmuller J, Moffatt M, Wardlaw A, Parker S, Connolly M, Koppelman G, Sayers I
FASEB J, 2013-11-18;28(2):923-34.
Applications: Bioassay -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Applications: ELISA (Standard)
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