Media and Supplements for Mesenchymal Stem Cells

StemXVivo™ Media for Mesenchymal Cell Culture

Culture media specifically optimized for use with mesenchymal stem cells.

Researchers employing stem or progenitor cells in their studies currently use a wide variety of media products to support their cultures. These media are generally developed or validated for cells other than stem cells, thus making them less desirable than cell type-specific media. To address this need, R&D Systems offers StemXVivo media products specifically designed for stem/progenitor cell culture. All the components included within these defined basal formulations have been selected, optimized, and validated for mesenchymal stem cell culture.

StemXVivo Freezing Media

Serum-Free MSC Freezing Media (Catalog # CCM016)

Freezing media for human/mouse/rat MSC cryopreservation

StemXVivo Base Media

Serum-Containing MSC Expansion Media (Catalog # CCM004) and Serum-Free Human MSC Expansion Media (Catalog # CCM014)

A FBS-containing (Catalog # CCM004) or serum-free complete media (Catalog # CCM014) for the expansion of mesenchymal stem cells (MSCs).

Chondrogenic Base Media (Catalog # CCM005)

Base media for the differentiation of MSCs into chrondrocytes. For use with StemXVivo Chondrogenic Supplement.

Osteogenic/Adipogenic Base Media (Catalog # CCM007)

Base media for the differentiation of MSCs into osteocytes or adipocytes. For use with StemXVivo Osteogenic or Adipogenic Supplement.

StemXVivo Media Supplements

Adipogenic Supplement (Catalog # CCM011)

Media supplement designed to optimize the differentiation of MSCs into adipocytes.

Chondrogenic Supplement (Catalog # CCM006)

Media supplement designed to optimize the differentiation of MSCs into chrondrocytes.

Human Osteogenic Supplement (Catalog # CCM008) or Mouse/Rat Osteogenic Supplement (Catalog # CCM009)

Media supplement designed to optimize the differentiation of human or mouse MSCs into osteocytes.

Phenotypic Analysis of Human MSCs by Flow Cytometry

Phenotypic Analysis of Human MSCs by Flow Cytometry. MSCs were expanded using StemXVivo Mesenchymal Stem Cell Expansion Media (Catalog # CCM004). Gray histograms indicate cells stained for markers of undifferentiated MSCs using a PE-conjugated Mouse Anti-Human CD105 Monoclonal Antibody (Catalog # FAB10971P) or a PE-conjugated Mouse Anti-Human CD90 Monoclonal Antibody (Catalog # FAB2067P). The open histograms show isotype-matched control staining. MSCs lack expression of CD34.