Home / CCL5/RANTES / Mouse CCL5/RANTES DuoSet ELISA

Mouse CCL5/RANTES DuoSet ELISA

Catalog #: DY478
85 Citations 12 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY478
DY478-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse CCL5 / RANTES ELISA Standard Curve
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Product Details
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Citations (85)
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Supplemental Products
Reviews (12)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse CCL5/RANTES DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CCL5/RANTES. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse CCL5 / RANTES ELISA Standard Curve View Larger

Mouse CCL5 / RANTES ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell-expressed, and presumably Secreted), also known as CCL5, a member of the CC chemokine family of inflammatory and immunoregulatory cytokines, initially discovered by subtractive hybridization as a T cell-specific molecule (1, 2). Mouse RANTES cDNA encodes a 91 amino acid (aa) residue precursor protein with a presumed signal peptide of 23 aa residues that is cleaved to generate the 68 aa residue mature protein (3). At the amino acid sequence level, mouse RANTES is 84% identical to human RANTES. Both human and mouse RANTES exhibit cross species activity. Naturally occurring human RANTES has been found to be a mixture of the 68 aa residue mature protein and a 66 aa residue amino-terminally truncated form (4, 5). Cells known to express RANTES include keratinocytes, eosinophils, platelets, endothelium, fibroblasts, respiratory epithelium, astrocytes, vascular smooth muscle, and CD4+ and CD8+ T cells (6-14). As suggested by its acronym, RANTES production by the various cell types is induced in response to cytokine stimulation (8-13). 

Like other CC chemokines, RANTES is a monocyte chemoattractant (2). RANTES can also chemoattract unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+ T cells with the naive and memory phenotypes. In addition, RANTES can chemoattract and degranulate eosinophils, as well as chemoattract and induce histamine release from basophils. Human RANTES has been shown to be an inhibitor of HIV infection of human mononuclear cells (15, 16). Several CC chemokine receptors, including CCR-1, CCR-3, CCR-4, and CCR-5, have been shown to bind RANTES and subsequently to transduce a signal by increasing the intracellular calcium ion level (2, 17-21).

Entrez Gene IDs:
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names:
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse CCL5/RANTES DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

85 Citations: Showing 1 - 10
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  1. Intravenous administration of BCG in mice promotes natural killer and T cell-mediated antitumor immunity in the lung
    Authors: Moreo, E;Jarit-Cabanillas, A;Robles-Vera, I;Uranga, S;Guerrero, C;Gómez, AB;Mata-Martínez, P;Minute, L;Araujo-Voces, M;Felgueres, MJ;Esteso, G;Uranga-Murillo, I;Arias, M;Pardo, J;Martín, C;Valés-Gómez, M;Del Fresno, C;Sancho, D;Aguiló, N;
    Nature communications
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. Chemokine receptor 5 signaling in PFC mediates stress susceptibility in female mice
    Authors: Lin, HY;Cathomas, F;Li, L;Cuttoli, RD;Guevara, C;Bayrak, CS;Wang, Q;Gupta, S;Chan, KL;Shimo, Y;Parise, LF;Yuan, C;Aubry, AV;Chen, F;Wong, J;Morel, C;Huntley, GW;Zhang, B;Russo, SJ;Wang, J;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Plasma
  3. Coenzyme Q10 exhibits anti-inflammatory and immune-modulatory thereby decelerating the occurrence of experimental cerebral malaria
    Authors: Nyariki, JN;Kimani, NM;Kibet, PS;Kinuthia, GK;Isaac, AO;
    Molecular and biochemical parasitology
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Potential of Nucleic Acid Receptor Ligands to Improve Vaccination Efficacy against the Filarial Nematode Litomosoides sigmodontis
    Authors: Scheunemann, JF;Risch, F;Reichwald, JJ;Lenz, B;Neumann, AL;Garbe, S;Frohberger, SJ;Koschel, M;Ajendra, J;Rothe, M;Latz, E;Coch, C;Hartmann, G;Schumak, B;Hoerauf, A;H�bner, MP;
    Vaccines
    Species: Mouse
    Sample Types: Cell Culture Supernates, Pleural Lavage Fluid
  5. Salmonella Type III Secretion Effector SrfJ: A Glucosylceramidase Affecting the Lipidome and the Transcriptome of Mammalian Host Cells
    Authors: Aguilera-Herce, J;Panadero-Medianero, C;S�nchez-Romero, MA;Balbont�n, R;Bernal-Bayard, J;Ramos-Morales, F;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. STING-dependent interferon signatures restrict osteoclast differentiation and bone loss in mice
    Authors: S MacLauchla, P Kushwaha, A Tai, J Chen, C Manning, G Swarnkar, Y Abu-Amer, KA Fitzgerald, S Sharma, EM Gravallese
    Proceedings of the National Academy of Sciences of the United States of America, 2023-04-06;120(15):e2210409120.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. PYHIN protein IFI207 regulates cytokine transcription and IRF7 and contributes to the establishment of K. pneumoniae infection
    Authors: M Baran, C Feriotti, A McGinley, SR Carlile, Z Jiang, R Calderon-G, A Dumigan, J Sá-Pessoa, CE Sutton, J Kearney, RM McLoughlin, KHG Mills, KA Fitzgerald, JA Bengeochea, AG Bowie
    Cell Reports, 2023-04-04;42(4):112341.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism
    Authors: D Levy, SN Abadchi, N Shababi, MR Ravari, NH Pirolli, C Bergeron, A Obiorah, F Mokhtari-E, S Gheshlaghi, JM Abraham, IM Smith, E Powsner, T Solomon, JW Harmon, SM Jay
    bioRxiv : the preprint server for biology, 2023-03-23;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. MYC promotes immune-suppression in triple-negative breast cancer via inhibition of interferon signaling
    Authors: D Zimmerli, CS Brambillas, F Talens, J Bhin, R Linstra, L Romanens, A Bhattachar, SEP Joosten, AM Da Silva, N Padrao, MD Wellenstei, K Kersten, M de Boo, M Roorda, L Henneman, R de Bruijn, S Annunziato, E van der Bu, AP Drenth, C Lutz, T Endres, M van de Ven, M Eilers, L Wessels, KE de Visser, W Zwart, RSN Fehrmann, MATM van Vugt, J Jonkers
    Nature Communications, 2022-11-02;13(1):6579.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Protective and vaccine dose-sparing efficacy of Poly I:C-functionalized calcium phosphate nanoparticle adjuvants in inactivated influenza vaccination
    Authors: J Lee, SY Ahn, CTT Le, DH Lee, J Jung, EJ Ko
    International immunopharmacology, 2022-09-14;112(0):109240.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways
    Authors: M Pereira, DF Durso, CE Bryant, EA Kurt-Jones, N Silverman, DT Golenbock, RT Gazzinelli
    Cell Reports, 2022-08-16;40(7):111225.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Kupffer Cells and Blood Monocytes Orchestrate the Clearance of Iron-Carbohydrate Nanoparticles from Serum
    Authors: T Arsiwala, AS Vogt, AE Barton, V Manolova, F Funk, B Flühmann, MF Bachmann
    International Journal of Molecular Sciences, 2022-02-28;23(5):.
    Species: Mouse
    Sample Types: Tissue Lysates
  13. Heat-Inactivated Akkermansia muciniphila Improves Gut Permeability but Does Not Prevent Development of Non-Alcoholic Steatohepatitis in Diet-Induced Obese Ldlr-/-.Leiden Mice
    Authors: MC Morrison, E Gart, WV Duyvenvoor, J Snabel, MJ Nielsen, DJ Leeming, A Menke, R Kleemann
    International Journal of Molecular Sciences, 2022-02-19;23(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury
    Authors: MH Ho, CH Yen, TH Hsieh, TJ Kao, JY Chiu, YH Chiang, BJ Hoffer, WC Chang, SY Chou
    Redox Biology, 2021-07-17;46(0):102067.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Ligase Pellino3 Regulates Macrophage Action and Survival in Response to VSV Infection in RIG-I-Dependent Path
    Authors: P Reniewicz, A Kula, E Makuch, M Ochnik, T Lipi?ski, J Siednienko
    Oxidative Medicine and Cellular Longevity, 2021-07-01;2021(0):6668463.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Resveratrol-Loaded Lipid-Core Nanocapsules Modulate Acute Lung Inflammation and Oxidative Imbalance Induced by LPS in Mice
    Authors: MTP de Oliveir, DS Coutinho, SS Guterres, AR Pohlmann, PMRE Silva, MA Martins, A Bernardi
    Pharmaceutics, 2021-05-10;13(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Combination of subtherapeutic anti-TNF dose with dasatinib restores clinical and molecular arthritogenic profiles better than standard anti-TNF treatment
    Authors: L Ntari, C Nikolaou, K Kranidioti, D Papadopoul, E Christodou, P Chouvardas, F Meier, C Geka, MC Denis, N Karagianni, G Kollias
    Journal of Translational Medicine, 2021-04-23;19(1):165.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation
    Authors: E Mai, CM Percopo, AR Limkar, AC Sek, M Ma, HF Rosenberg
    Viruses, 2020-12-22;13(1):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. Leptospiral LPS escapes mouse TLR4 internalization and TRIF?associated antimicrobial responses through O antigen and associated lipoproteins
    Authors: D Bonhomme, I Santecchia, F Vernel-Pau, M Caroff, P Germon, G Murray, B Adler, IG Boneca, C Werts
    PLoS Pathog., 2020-08-13;16(8):e1008639.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Inhibition of Vps34 reprograms cold into hot inflamed tumors and improves anti-PD-1/PD-L1 immunotherapy
    Authors: MZ Noman, S Parpal, K Van Moer, M Xiao, Y Yu, J Viklund, A De Milito, M Hasmim, M Andersson, RK Amaravadi, J Martinsson, G Berchem, B Janji
    Sci Adv, 2020-04-29;6(18):eaax7881.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. Pre-treatment with the viral Toll-like receptor 3 agonist poly(I:C) modulates innate immunity and protects neutropenic mice infected intracerebrally with Escherichia coli
    Authors: S Ribes, C Arcilla, M Ott, S Schütze, UK Hanisch, S Nessler, R Nau
    J Neuroinflammation, 2020-01-17;17(1):24.
    Species: Mouse
    Sample Types: Tissue homoge
  22. The alternative cap-binding complex is required for antiviral defense in vivo
    Authors: A Gebhardt, V Bergant, D Schnepf, M Moser, A Meiler, D Togbe, C Mackowiak, LS Reinert, SR Paludan, B Ryffel, A Stukalov, P Staeheli, A Pichlmair
    PLoS Pathog., 2019-12-19;15(12):e1008155.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. PTP1B negatively regulates nitric oxide-mediated Pseudomonas aeruginosa killing by neutrophils
    Authors: L Yue, M Yan, ML Tremblay, TJ Lin, H Li, T Yang, X Song, T Xie, Z Xie
    PLoS ONE, 2019-09-18;14(9):e0222753.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. In-depth characterization of congenital Zika syndrome in immunocompetent mice: Antibody-dependent enhancement and an antiviral peptide therapy
    Authors: VN Camargos, G Foureaux, DC Medeiros, VT da Silveir, CM Queiroz-Ju, ALB Matosinhos, AFA Figueiredo, CDF Sousa, TP Moreira, VF Queiroz, ACF Dias, KTO Santana, I Passos, ALCV Real, LC Silva, FAG Mourão, NT Wnuk, MAP Oliveira, S Macari, T Silva, GP Garlet, JA Jackman, FM Soriani, MFD Moraes, EMAM Mendes, FM Ribeiro, GMJ Costa, AL Teixeira, NJ Cho, ACP Oliveira, MM Teixeira, VV Costa, DG Souza
    EBioMedicine, 2019-05-23;0(0):.
    Species: Mouse
    Sample Types: Plasma
  25. Innate immune memory through TLR2 and NOD2 contributes to the control of Leptospira interrogans infection
    Authors: I Santecchia, F Vernel-Pau, O Rasid, J Quintin, M Gomes-Sole, IG Boneca, C Werts
    PLoS Pathog., 2019-05-20;15(5):e1007811.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Translational repression of Ccl5 and Cxcl10 by 4E-BP1 and 4E-BP2 restrains the ability of mouse macrophages to induce migration of activated T�cells
    Authors: M William, LP Leroux, V Chaparro, TE Graber, T Alain, M Jaramillo
    Eur. J. Immunol., 2019-05-06;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Role of a fluid-phase PRR in fighting an intracellular pathogen: PTX3 in Shigella infection
    Authors: V Ciancarell, L Lembo-Fazi, I Paciello, AK Bruno, S Jaillon, S Berardi, M Barbagallo, S Meron-Suda, D Cohen, A Molinaro, G Rossi, C Garlanda, ML Bernardini
    PLoS Pathog., 2018-12-07;14(12):e1007469.
    Species: Mouse
    Sample Types: Tissue Homogenates
  28. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma
    Authors: GM Wiedemann, C Aithal, A Kraechan, C Heise, BL Cadilha, J Zhang, P Duewell, R Ballotti, S Endres, C Bertolotto, S Kobold
    Transl Oncol, 2018-11-28;12(2):350-360.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. MiR-188-3p upregulation results in the inhibition of macrophage proinflammatory activities and atherosclerosis in ApoE-deficient mice
    Authors: XF Zhang, Y Yang, XY Yang, Q Tong
    Thromb. Res., 2018-09-05;171(0):55-61.
    Species: Mouse
    Sample Types: Plasma
  30. Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations
    Authors: A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston
    Nat Commun, 2018-06-08;9(1):2229.
    Species: Mouse
    Sample Types: BALF
  31. Comparing Flow Cytometry QBeads PlexScreen Assays with Other Immunoassays for Determining Multiple Analytes
    Authors: M Ding, A Cavallin, NO Hermansson, P Berntsson, L Jinton, S Rodrigo Bl
    SLAS Discov, 2018-04-24;0(0):2472555218771.
    Species: Mouse
    Sample Types: BALF
  32. Genetic Polymorphism at CCL5 Is Associated With Protection in Chagas' Heart Disease: Antagonistic Participation of CCR1+ and CCR5+ Cells in Chronic Chagasic Cardiomyopathy.
    Authors: Angelica Martins Batista, Lucia Elena Alvarado-, Silvia Marinho Alves, Gloria Melo, Isabela Resende Pereira, Leonardo Alexandre de Sou Ruivo, Andrea Alice Da Silva, Daniel Gibaldi, Thayse Do E S Protásio Da Silva, Virginia Maria Barros De Lorena, Adriene Siqueira De Melo, Ana Karine De Araújo, Michelle Da Silva Barros, Vláudia Maria Assis Costa, Cynthia C Cardoso, Antonio G Pacheco, Cristina Carrazzon, Wilson Oliveira, Milton Ozório Moraes, Joseli Lannes-Vi
    Frontiers in Immunology, 2018-04-11;0(0):1664-3224.
    Species: Mouse
    Sample Types: Tissue Homogenates
  33. Chronic Intake of the Selective Serotonin Reuptake Inhibitor Fluoxetine Enhances Atherosclerosis
    Authors: M Rami, R Guillamat-, P Rinne, M Salvermose, L Ring, M Bianchini, X Blanchet, RTA Megens, Y Döring, B Walzog, O Soehnlein, C Weber, A Faussner, S Steffens
    Arterioscler. Thromb. Vasc. Biol., 2018-03-22;0(0):.
    Species: Mouse
    Sample Types: Serum
  34. Bcl11b, a novel GATA3-interacting protein, suppresses Th1 while limiting Th2 cell differentiation
    Authors: D Fang, K Cui, G Hu, RK Gurram, C Zhong, AJ Oler, R Yagi, M Zhao, S Sharma, P Liu, B Sun, K Zhao, J Zhu
    J. Exp. Med., 2018-03-07;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection
    Authors: EE Hornick, B Banoth, AM Miller, ZR Zacharias, N Jain, ME Wilson, KN Gibson-Cor, KL Legge, GA Bishop, FS Sutterwala, SL Cassel
    J. Immunol., 2017-12-27;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  36. Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia
    Authors: S Paetau, T Rolova, L Ning, CG Gahmberg
    Front Mol Neurosci, 2017-12-22;10(0):431.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  37. Cathelicidins Inhibit Escherichia coli-Induced TLR2 and TLR4 Activation in a Viability-Dependent Manner
    Authors: M Coorens, VAF Schneider, AM de Groot, A van Dijk, M Meijerink, JM Wells, MR Scheenstra, EJA Veldhuizen, HP Haagsman
    J. Immunol., 2017-07-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. The Deep-Sea Polyextremophile Halobacteroides lacunaris TB21 Rough-Type LPS: Structure and Inhibitory Activity towards Toxic LPS
    Authors: FD Lorenzo, A Palmigiano, I Paciello, M Pallach, D Garozzo, ML Bernardini, V Cono, MM Yakimov, A Molinaro, A Silipo
    Mar Drugs, 2017-06-27;15(7):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Interleukin 17A Promotes Lymphocytes Adhesion and Induces CCL2 and CXCL1 Release from Brain Endothelial Cells
    Authors: DW Wojkowska, P Szpakowski, A Glabinski
    Int J Mol Sci, 2017-05-08;18(5):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. Protein malnutrition promotes dysregulation of molecules involved in T cell migration in the thymus of mice infected with Leishmania infantum
    Authors: M Losada-Bar, A Umaña-Pére, S Cuervo-Esc, LR Berbert, R Porrozzi, FN Morgado, DA Mendes-da-, W Savino, M Sánchez-Gó, P Cuervo
    Sci Rep, 2017-04-11;7(0):45991.
    Species: Mouse
    Sample Types: Interstitial Fluid
  41. Contributions of CD8 T cells to the pathogenesis of mouse adenovirus type 1 respiratory infection
    Authors: CT Molloy, JS Andonian, HM Seltzer, MC Procario, ME Watson, JB Weinberg
    Virology, 2017-04-11;507(0):64-74.
    Species: Mouse
    Sample Types: BALF
  42. Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants
    Authors: LT Hsieh, MV Nastase, H Roedig, J Zeng-Brouw, C Poluzzi, S Schwalm, C Fork, C Tredup, RP Brandes, M Wygrecka, A Huwiler, J Pfeilschif, L Schaefer
    Int J Mol Sci, 2017-03-09;18(3):.
    Species: Human
    Sample Types: Plasma
  43. The protective effect of the anti-Toll-like receptor 9 antibody against acute cytokine storm caused by immunostimulatory DNA
    Authors: Y Murakami, R Fukui, Y Motoi, T Shibata, SI Saitoh, R Sato, K Miyake
    Sci Rep, 2017-03-07;7(0):44042.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  44. Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation
    Authors: YT Chu, SW Wan, YC Chang, CK Lee, BA Wu-Hsieh, R Anderson, YS Lin
    Lab. Invest, 2017-02-27;0(0):.
    Species: Mouse
    Sample Types: Serum
  45. The TRAIL-Induced Cancer Secretome Promotes a Tumor-Supportive Immune Microenvironment via CCR2
    Authors: T Hartwig, A Montinaro, S von Karste, A Sevko, S Surinova, A Chakravart, L Taraborrel, P Draber, E Lafont, F Arce Varga, MA El-Bahrawy, SA Quezada, H Walczak
    Mol. Cell, 2017-02-16;65(4):730-742.e5.
    Species: Mouse
    Sample Types: Tissue Homogenates
  46. Deletion of the Chemokine Binding Protein Gene from the Parapoxvirus Orf Virus Reduces Virulence and Pathogenesis in Sheep
    Authors: SB Fleming, C McCaughan, Z Lateef, A Dunn, LM Wise, NC Real, AA Mercer
    Front Microbiol, 2017-01-24;8(0):46.
    Species: Mouse
    Sample Types: Protein
  47. Interspecies cathelicidin comparison reveals divergence in antimicrobial activity, TLR modulation, chemokine induction and regulation of phagocytosis
    Authors: M Coorens, MR Scheenstra, EJ Veldhuizen, HP Haagsman
    Sci Rep, 2017-01-19;7(0):40874.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  48. NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis
    Authors: Andrey Lozhkin
    J. Mol. Cell. Cardiol, 2016-12-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. Protease-Activated Receptor 1 Enhances Poly I:C Induction of the Antiviral Response in Macrophages and Mice
    Authors: Nigel Mackman
    J Innate Immun, 2016-11-08;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  50. P2X7 receptor-dependent tuning of gut epithelial responses to infection
    Authors: SW Huang, C Walker, J Pennock, K Else, W Muller, MJ Daniels, C Pellegrini, D Brough, G Lopez-Cast, SM Cruickshan
    Immunol. Cell Biol, 2016-08-25;95(2):178-188.
    Species: Mouse
    Sample Types: Serum
  51. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.
    Authors: Brown A, Murphy A, Lalor S, Leech J, O'Keeffe K, Mac Aogain M, O'Halloran D, Lacey K, Tavakol M, Hearnden C, Fitzgerald-Hughes D, Humphreys H, Fennell J, van Wamel W, Foster T, Geoghegan J, Lavelle E, Rogers T, McLoughlin R
    PLoS Pathog, 2015-11-05;11(11):e1005226.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  52. Ethanolic Echinacea purpurea Extracts Contain a Mixture of Cytokine-Suppressive and Cytokine-Inducing Compounds, Including Some That Originate from Endophytic Bacteria.
    Authors: Todd D, Gulledge T, Britton E, Oberhofer M, Leyte-Lugo M, Moody A, Shymanovich T, Grubbs L, Juzumaite M, Graf T, Oberlies N, Faeth S, Laster S, Cech N
    PLoS ONE, 2015-05-01;10(5):e0124276.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases.
    Authors: Sottnik J, Dai J, Zhang H, Campbell B, Keller E
    Cancer Res, 2015-04-08;75(11):2151-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  54. Phosphatidylinositol 3-Kinase gamma is required for the development of experimental cerebral malaria.
    Authors: Lacerda-Queiroz N, Brant F, Rodrigues D, Vago J, Rachid M, Sousa L, Teixeira M, Teixeira A
    PLoS ONE, 2015-03-16;10(3):e0119633.
    Species: Mouse
    Sample Types: Serum
  55. Dietary galacto-oligosaccharides prevent airway eosinophilia and hyperresponsiveness in a murine house dust mite-induced asthma model.
    Authors: Verheijden K, Willemsen L, Braber S, Leusink-Muis T, Delsing D, Garssen J, Kraneveld A, Folkerts G
    Respir Res, 2015-02-07;16(0):17.
    Species: Mouse
    Sample Types: Tissue Homogenates
  56. Necroptosis suppresses inflammation via termination of TNF- or LPS-induced cytokine and chemokine production.
    Authors: Kearney C, Cullen S, Tynan G, Henry C, Clancy D, Lavelle E, Martin S
    Cell Death Differ, 2015-01-23;22(8):1313-27.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  57. Interleukin-15 modulates adipose tissue by altering mitochondrial mass and activity.
    Authors: Barra, Nicole G, Palanivel, Rengasam, Denou, Emmanuel, Chew, Marianne, Gillgrass, Amy, Walker, Tina D, Kong, Josh, Richards, Carl D, Jordana, Manel, Collins, Stephen, Trigatti, Bernardo, Holloway, Alison C, Raha, Sandeep, Steinberg, Gregory, Ashkar, Ali A
    PLoS ONE, 2014-12-17;9(12):e114799.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. The Fli-1 transcription factor regulates the expression of CCL5/RANTES.
    Authors: Lennard Richard M, Sato S, Suzuki E, Williams S, Nowling T, Zhang X
    J Immunol, 2014-08-06;193(6):2661-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  59. Animal model of respiratory syncytial virus: CD8+ T cells cause a cytokine storm that is chemically tractable by sphingosine-1-phosphate 1 receptor agonist therapy.
    Authors: Walsh K, Teijaro J, Brock L, Fremgen D, Collins P, Rosen H, Oldstone M
    J Virol, 2014-03-26;88(11):6281-93.
    Species: Mouse
    Sample Types: BALF
  60. Mapping the innate signaling cascade essential for cytokine storm during influenza virus infection.
    Authors: Teijaro J, Walsh K, Rice S, Rosen H, Oldstone M
    Proc Natl Acad Sci U S A, 2014-02-26;111(10):3799-804.
    Species: Mouse
    Sample Types: BALF
  61. Lack of platelet-activating factor receptor protects mice against diet-induced adipose inflammation and insulin-resistance despite fat pad expansion.
    Authors: Menezes-Garcia Z, Oliveira M, Lima R, Soriani F, Cisalpino D, Botion L, Teixeira M, Souza D, Ferreira A
    Obesity (Silver Spring), 2013-12-14;22(3):663-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Authors: Paulissen G, El Hour M, Rocks N, Gueders M, Bureau F, Foidart J, Lopez-Otin C, Noel A, Cataldo D
    J Immunol, 2012-09-07;189(8):4135-43.
    Species: Mouse
    Sample Types: Tissue Homogenates
  63. Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, Is crucial for murine asthma.
    Authors: Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Korner H, Cua D, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide D, Matsumoto K, Nakae S
    J Immunol, 2012-08-31;189(7):3641-52.
    Species: Mouse
    Sample Types: BALF
  64. Exosomes isolated from mycobacteria-infected mice or cultured macrophages can recruit and activate immune cells in vitro and in vivo.
    Authors: Singh PP, Smith VL, Karakousis PC, Schorey JS
    J. Immunol., 2012-06-20;189(2):777-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  65. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
    Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
    PLoS ONE, 2012-02-29;7(2):e32125.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  66. Identification and functional characterization of novel phosphorylation sites in TAK1-binding protein (TAB) 1.
    Authors: Wolf A, Beuerlein K, Eckart C, Weiser H, Dickkopf B, Muller H, Sakurai H, Kracht M
    PLoS ONE, 2011-12-22;6(12):e29256.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  67. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2011-10-24;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  68. Endothelial cells are central orchestrators of cytokine amplification during influenza virus infection.
    Authors: Teijaro JR, Walsh KB, Cahalan S, Fremgen DM, Roberts E, Scott F, Martinborough E, Peach R, Oldstone MB, Rosen H
    Cell, 2011-09-16;146(6):980-91.
    Species: Mouse
    Sample Types: BALF
  69. Suppression of cytokine storm with a sphingosine analog provides protection against pathogenic influenza virus.
    Authors: Walsh KB, Teijaro JR, Wilker PR
    Proc. Natl. Acad. Sci. U.S.A., 2011-06-29;108(29):12018-23.
    Species: Mouse
    Sample Types: BALF
  70. Chemokine binding protein M3 of murine gammaherpesvirus 68 modulates the host response to infection in a natural host.
    Authors: Hughes DJ, Kipar A, Leeming GH, Bennett E, Howarth D, Cummerson JA, Papoula-Pereira R, Flanagan BF, Sample JT, Stewart JP
    PLoS Pathog., 2011-03-17;7(3):e1001321.
    Species: Mouse
    Sample Types: Tissue Homogenates
  71. Inflammatory chemokine release of astrocytes in vitro is reduced by all-trans retinoic acid.
    Authors: van Neerven S, Regen T, Wolf D, Nemes A, Johann S, Beyer C, Hanisch UK, Mey J
    J. Neurochem., 2010-06-16;114(5):1511-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  72. Chorionic gonadotropin alleviates thioglycollate-induced peritonitis by affecting macrophage function.
    Authors: Wan H, Coppens JM, van Helden-Meeuwsen CG, Leenen PJ, Van Rooijen N, Khan NA, Kiekens RC, Benner R, Versnel MA
    J. Leukoc. Biol., 2009-05-04;86(2):361-70.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  73. IFN-gamma synergizes with LPS to induce nitric oxide biosynthesis through glycogen synthase kinase-3-inhibited IL-10.
    Authors: Lin CF, Tsai CC, Huang WC, Wang CY, Tseng HC, Wang Y, Kai JI, Wang SW, Cheng YL
    J. Cell. Biochem., 2008-10-15;105(3):746-55.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  74. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008-03-26;28(13):3277-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  75. Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection.
    Authors: Macedo GC, Magnani DM, Carvalho NB, Bruna-Romero O, Gazzinelli RT, Oliveira SC
    J. Immunol., 2008-01-15;180(2):1080-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  76. Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes.
    Authors: Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE
    J. Immunol., 2007-12-15;179(12):8381-91.
    Species: Human
    Sample Types: Cell Culture Supernates
  77. Differential ex vivo nitric oxide production by acutely isolated neonatal and adult microglia.
    Authors: Schell JB, Crane CA, Smith MF, Roberts MR
    J. Neuroimmunol., 2007-08-15;189(1):75-87.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. Lipoxin A4 stable analogs reduce allergic airway responses via mechanisms distinct from CysLT1 receptor antagonism.
    Authors: Levy BD, Lukacs NW, Berlin AA, Schmidt B, Guilford WJ, Serhan CN, Parkinson JF
    FASEB J., 2007-07-11;21(14):3877-84.
    Species: Mouse
    Sample Types: Tissue Homogenates
  79. Neurokinin-1 receptor antagonist treatment protects mice against lung injury in polymicrobial sepsis.
    Authors: Hegde A, Zhang H, Moochhala SM, Bhatia M
    J. Leukoc. Biol., 2007-06-12;82(3):678-85.
    Species: Mouse
    Sample Types: Plasma
  80. Interleukin-17 is a negative regulator of established allergic asthma.
    Authors: Schnyder-Candrian S, Togbe D, Couillin I, Mercier I, Brombacher F, Quesniaux V, Fossiez F, Ryffel B, Schnyder B
    J. Exp. Med., 2006-11-13;203(12):2715-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  81. Suppressive effect of simvastatin on interferon-beta-induced expression of CC chemokine ligand 5 in microglia.
    Authors: Nakamichi K, Saiki M, Kitani H, Kuboyama Y, Morimoto K, Takayama-Ito M, Kurane I
    Neurosci. Lett., 2006-09-15;407(3):205-10.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  82. Mucosal administration of CpG oligodeoxynucleotide elicits strong CC and CXC chemokine responses in the vagina and serves as a potent Th1-tilting adjuvant for recombinant gD2 protein vaccination against genital herpes.
    Authors: Tengvall S, Lundqvist A, Eisenberg RJ, Cohen GH, Harandi AM
    J. Virol., 2006-06-01;80(11):5283-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
  83. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004-08-01;173(3):2084-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  84. Specific engagement of TLR4 or TLR3 does not lead to IFN-beta-mediated innate signal amplification and STAT1 phosphorylation in resident murine alveolar macrophages.
    Authors: Punturieri A, Alviani RS, Polak T, Copper P, Sonstein J, Curtis JL
    J. Immunol., 2004-07-15;173(2):1033-42.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  85. Orally administered CpG oligodeoxynucleotide induces production of CXC and CC chemokines in the gastric mucosa and suppresses bacterial colonization in a mouse model of Helicobacter pylori infection.
    Authors: Raghavan S, Nystrom J, Fredriksson M, Holmgren J, Harandi AM
    Infect. Immun., 2003-12-01;71(12):7014-22.
    Species: Mouse
    Sample Types: Serum

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Reviews for Mouse CCL5/RANTES DuoSet ELISA

Average Rating: 4.9 (Based on 12 Reviews)

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Mouse CCL5/RANTES DuoSet ELISA
By Anonymous on 11/10/2019
Sample Tested: Cell Culture Supernates,RAW 264.7 mouse monocyte/macrophage cell line
Mouse CCL5/RANTES DuoSet ELISA DY478
Mouse CCL5/RANTES DuoSet ELISA
By Anonymous on 08/10/2019
Sample Tested: Cell Lysates
Mouse CCL5/RANTES DuoSet ELISA DY478
Mouse CCL5/RANTES DuoSet ELISA
By Anonymous on 04/15/2019
Sample Tested: peritoneal lavage fluid

Mice (HIII and LIII strains) injected i.p. with 2,4,6,10 tetramethylpentadecane (pristane).

Mouse CCL5/RANTES DuoSet ELISA DY478
Mouse CCL5/RANTES DuoSet ELISA
By Anonymous on 10/08/2018
Sample Tested: Serum
Mouse CCL5/RANTES DuoSet ELISA DY478
Mouse CCL5/RANTES DuoSet ELISA
By Anonymous on 05/14/2018
Sample Tested: Min6 mouse insulinoma whole cell lysate
Mouse CCL5/RANTES DuoSet ELISA
By Anonymous on 09/27/2017
Sample Tested: Peritoneal resident macrophages
Mouse CCL5/RANTES DuoSet ELISA
By Goutham Pattabiraman on 04/21/2017
Sample Tested: Cell culture supernatant,Serum
Mouse CCL5/RANTES DuoSet ELISA DY478
Mouse CCL5/RANTES DuoSet ELISA
By Anonymous on 04/21/2017

used for ELISA, worked perfectly.

Mouse CCL5/RANTES DuoSet ELISA
By Daniel Zhang on 04/19/2017
Sample Tested: Lung tissue
Mouse CCL5/RANTES DuoSet ELISA
By Anonymous on 10/06/2016
Sample Tested: Cell culture supernatant
Mouse CCL5/RANTES DuoSet ELISA DY478
Mouse CCL5/RANTES DuoSet ELISA
By Austin Russon on 06/03/2016
Sample Tested: Blood Serum,Cell Culture Supernates
Mouse CCL5/RANTES DuoSet ELISA, 15 Plate
By Anonymous on 10/26/2015
Sample Tested: Purified mouse RANTES protein

50ul of murine RANTES Capture antibody (2ug/mL) was coated per well of a 96 well plate at 4 degrees overnight. Purified RANTES standard was diluted (7 fold) and assayed to ensure the quality of the ELISA. Mouse RANTES Detector antibody was added (2h @ RT) followed by a 20 min incubation with Streptavidin-HRP. Substrate solution was added to measure RANTES concentration according to the manufactures protocol. The standard curve looked great as can be seen by calculation of the R- squared value. Highly recommend doing ELISA's using kits from R&D.
Buffer: 7-point standard curve

Mouse CCL5/RANTES DuoSet ELISA, 15 Plate DY478