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Mouse Complement Factor D/Adipsin DuoSet ELISA

Catalog #: DY5430-05
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY5430-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse Complement Factor D / Adipsin ELISA Standard Curve
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse Complement Factor D/Adipsin DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
375.0 - 6,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Mouse Complement Factor D / Adipsin ELISA Standard Curve View Larger

Mouse Complement Factor D / Adipsin ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Complement Factor D/Adipsin

Complement Factor D, also known as adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3.3 Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors.

Entrez Gene IDs:
1675 (Human); 11537 (Mouse); 54249 (Rat)
Alternate Names:
Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN

Citations for Mouse Complement Factor D/Adipsin DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Adipocyte PI3K links adipostasis with baseline insulin secretion at fasting through an adipoincretin effect
    Authors: Becattini, B;Molinaro, A;Henricsson, M;Borén, J;Solinas, G;
    Cell reports
    Species: Viral
    Sample Types: Plasma
  2. Dried Bilberry (Vaccinium myrtillus L.) Alleviates the Inflammation and Adverse Metabolic Effects Caused by a High-Fat Diet in a Mouse Model of Obesity
    Authors: T Pemmari, M Hämäläinen, R Ryyti, R Peltola, E Moilanen
    International Journal of Molecular Sciences, 2022-09-20;23(19):.
    Species: Mouse
    Sample Types: Serum
  3. Congenital lipodystrophy induces severe osteosclerosis
    Authors: W Zou, N Rohatgi, JR Brestoff, Y Zhang, EL Scheller, CS Craft, MD Brodt, N Migotsky, MJ Silva, CA Harris, SL Teitelbaum
    PLoS Genet., 2019-06-24;15(6):e1008244.
    Species: Mouse
    Sample Types: Serum
  4. Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes
    Authors: M Rödiger, MW Werno, I Wilhelmi, C Baumeier, D Hesse, N Wettschure, S Offermanns, K Song, M Krau beta, A Schürmann
    Mol Metab, 2017-11-22;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates

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