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Mouse CXCL9/MIG Antibody

Catalog #: AF-492-NA
21 Citations Datasheet / COA / SDS
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AF-492-NA
AF-492-SP
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Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody.
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Citations (21)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL9/MIG Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL9/MIG in ELISAs. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) CXCL9/MIG is observed and less than 0.005% cross-reactivity with recombinant mouse MIP-2, recombinant rat CINC-1, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL9/MIG Accession # P18340
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse CXCL9/MIG Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CXCL9/MIG Biotinylated Antibody (Catalog # BAF492)

Standard: Recombinant Mouse CXCL9/MIG Protein (Catalog # 492-MM)

Neutralization
Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 6-20 µg/mL in the presence of 1 µg/mL Recombinant Mouse CXCL9/MIG.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody. View Larger

Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody. Recombinant Mouse CXCL9/MIG (Catalog # 492-MM) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND50 is typically 6-20 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: CXCL9/MIG

CXCL9, also known as MIG, is a member of the alpha subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

Entrez Gene IDs
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

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Citations for Mouse CXCL9/MIG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Inhibition of the CXCL9-CXCR3 axis suppresses the progression of experimental apical periodontitis by blocking macrophage migration and activation
    Authors: T Hasegawa, V Venkata Su, Y Yahata, M Nakano, S Suzuki, S Suzuki, S Yamada, H Kitaura, I Mizoguchi, Y Noiri, K Handa, M Saito
    Scientific Reports, 2021-01-28;11(1):2613.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Commensal-driven immune zonation of the liver promotes host defence
    Authors: A Gola, MG Dorrington, E Speranza, C Sala, RM Shih, AJ Radtke, HS Wong, AP Baptista, JM Hernandez, G Castellani, IDC Fraser, RN Germain
    Nature, 2020-11-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy
    Authors: M Pascual-Ga, E Bonfill-Te, E Planas-Rig, C Rubio-Pere, R Iurlaro, A Arias, I Cuartas, A Sala-Hojma, L Escudero, F Martínez-R, I Huber-Ruan, P Nuciforo, L Pedrosa, C Marques, I Braña, E Garralda, M Vieito, M Squatrito, E Pineda, F Graus, C Espejo, J Sahuquillo, J Tabernero, J Seoane
    Nat Commun, 2019-06-11;10(1):2416.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: ICC, Neutralization
  4. Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis
    Authors: N Koyanagi, T Imai, K Shindo, A Sato, W Fujii, T Ichinohe, N Takemura, S Kakuta, S Uematsu, H Kiyono, Y Maruzuru, J Arii, A Kato, Y Kawaguchi
    J. Clin. Invest., 2017-09-11;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  6. Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production
    Authors: P Shinde, W Liu, A Ménoret, AD Luster, AT Vella
    J. Leukoc. Biol., 2017-04-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Salt suppresses IFN? inducible chemokines through the IFN?-JAK1-STAT1 signaling pathway in proximal tubular cells
    Authors: Y Arai, D Takahashi, K Asano, M Tanaka, M Oda, SBH Ko, MSH Ko, S Mandai, N Nomura, T Rai, S Uchida, E Sohara
    Sci Rep, 2017-04-20;7(0):46580.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  8. Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone
    Nat Commun, 2016-12-14;7(0):13885.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo, Whole Cells
    Applications: Neutralization, Western Blot
  9. A Novel PD-L1-targeting Antagonistic DNA Aptamer With Antitumor Effects
    Authors: Wei-Yun Lai
    Mol Ther Nucleic Acids, 2016-12-13;5(12):e397.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
    J Immunol, 2016-07-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  11. Regulatory T cells prevent inducible BALT formation by dampening neutrophilic inflammation.
    Authors: Foo S, Zhang V, Lalwani A, Lynch J, Zhuang A, Lam C, Foster P, King C, Steptoe R, Mazzone S, Sly P, Phipps S
    J Immunol, 2015-03-25;194(9):4567-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Tumor cell-activated CARD9 signaling contributes to metastasis-associated macrophage polarization.
    Authors: Yang M, Shao J, Miao Y, Cui W, Qi Y, Han J, Lin X, Du J
    Cell Death Differ, 2014-04-11;21(8):1290-302.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Trafficking of high avidity HER-2/neu-specific T cells into HER-2/neu-expressing tumors after depletion of effector/memory-like regulatory T cells.
    Authors: Weiss VL, Lee TH, Song H, Kouo TS, Black CM, Sgouros G, Jaffee EM, Armstrong TD
    PLoS ONE, 2012-02-16;7(2):e31962.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. A novel combination immunotherapy for cancer by IL-13Ralpha2-targeted DNA vaccine and immunotoxin in murine tumor models.
    Authors: Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK
    J. Immunol., 2011-10-17;187(10):4935-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Monokine induced by interferon-gamma (MIG/CXCL9) is derived from both donor and recipient sources during rejection of class II major histocompatibility complex disparate skin allografts.
    Authors: Auerbach MB, Shimoda N, Amano H, Rosenblum JM, Kish DD, Farber JM, Fairchild RL
    Am. J. Pathol., 2009-04-23;174(6):2172-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells.
    Authors: Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martin-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007-05-27;8(7):743-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2006-10-18;81(2):783-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
    Authors: Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
    J. Immunol., 2004-02-01;172(3):1540-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
    Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM
    J. Exp. Med., 2001-11-05;194(9):1375-84.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization

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