Home / IL-2 / Mouse IL-2 DuoSet ELISA

Mouse IL-2 DuoSet ELISA

Catalog #: DY402
85 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY402
DY402-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse IL-2 ELISA Standard Curve
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Product Details
Procedure
Citations (85)
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Supplemental Products
Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IL-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Block Buffer: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Reagent Diluent: 0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

 

Scientific Data

N/A Mouse IL-2 ELISA Standard Curve View Larger

Mouse IL-2 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.

Long Name:
Interleukin 2
Entrez Gene IDs:
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names:
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of Block Buffer to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IL-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

85 Citations: Showing 1 - 10
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  1. The Tyrosine Phosphatase Activity of PTPN22 Is Involved in T Cell Development via the Regulation of TCR Expression
    Authors: Bai, B;Li, T;Zhao, J;Zhao, Y;Zhang, X;Wang, T;Zhang, N;Wang, X;Ba, X;Xu, J;Yu, Y;Wang, B;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Whole Blood
  2. Activation and differentiation of cognate T cells by a dextran-based antigen-presenting system for cancer immunotherapy
    Authors: Mahata, D;Mukherjee, D;Biswas, D;Basak, S;Basak, AJ;Jamir, I;Pandey, N;Khatoon, H;Samanta, D;Basak, A;Mukherjee, G;
    European journal of immunology
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Ligand-based adoptive T cell targeting CA125 in ovarian cancer
    Authors: Zhao, H;Wu, L;Dai, J;Sun, K;Zi, Z;Guan, J;Zhang, L;
    Journal of translational medicine
    Species: Mouse
    Sample Types: Cell Culture Suepernates
  4. IL-7R licenses a population of epigenetically poised memory CD8+ T cells with superior antitumor efficacy that are critical for melanoma memory
    Authors: Micevic, G;Daniels, A;Flem-Karlsen, K;Park, K;Talty, R;McGeary, M;Mirza, H;Blackburn, HN;Sefik, E;Cheung, JF;Hornick, NI;Aizenbud, L;Joshi, NS;Kluger, H;Iwasaki, A;Bosenberg, MW;Flavell, RA;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Modulation of tumor immune microenvironment by TAS-115, a multi-receptor tyrosine kinase inhibitor, promotes antitumor immunity and contributes anti-PD-1 antibody therapy
    Authors: Shibutani, T;Goto, R;Miyazaki, I;Hashimoto, A;Suzuki, T;Ishida, K;Haruma, T;Osada, T;Harada, T;Fujita, H;Ohkubo, S;
    Scientific reports
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Diversity-Oriented Synthesis of a Molecular Library of Immunomodulatory alpha-Galactosylceramides with Fluorous-Tag-Assisted Purification and Evaluation of Their Bioactivities in Regard to IL-2 Secretion
    Authors: YN Chen, JT Hung, FD Jan, YY Su, JR Hwu, AL Yu, AK Adak, CC Lin
    Oncogene, 2022-11-02;23(21):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Arginine-based cationic liposomes accelerate T cell activation and differentiation in vitro
    Authors: T Li, F Tolksdorf, W Sung, H Sato, FJ Eppler, M Hotta, W Kolanus, S Takeoka
    International journal of pharmaceutics, 2022-06-15;623(0):121917.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Menthone Inhalation Alleviates Local and Systemic Allergic Inflammation in Ovalbumin-Sensitized and Challenged Asthmatic Mice
    Authors: YH Su, JY Lin
    International Journal of Molecular Sciences, 2022-04-04;23(7):.
    Species: Mouse
    Sample Types: BALF
  9. Anti-CD80/86 antibodies inhibit inflammatory reaction and improve graft survival in a high-risk murine corneal transplantation rejection model
    Authors: J Zhu, T Inomata, M Nakamura, K Fujimoto, Y Akasaki, K Fujio, A Yanagawa, K Uchida, J Sung, N Negishi, K Nagino, Y Okumura, M Miura, H Shokirova, M Kuwahara, K Hirosawa, A Midorikawa, A Eguchi, T Huang, H Yagita, S Habu, K Okumura, A Murakami
    Scientific Reports, 2022-03-22;12(1):4853.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Target modulation and pharmacokinetics/pharmacodynamics translation of the BTK inhibitor poseltinib for model-informed phase II dose selection
    Authors: JY Byun, YT Koh, SY Jang, JW Witcher, JR Chan, A Pustilnik, MJ Daniels, YH Kim, KH Suh, MD Linnik, YM Lee
    Scientific Reports, 2021-09-21;11(1):18671.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. NR4A family members regulate T cell tolerance to preserve immune homeostasis and suppress autoimmunity
    Authors: R Hiwa, HV Nielsen, JL Mueller, R Mandla, J Zikherman
    JCI Insight, 2021-09-08;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Bifidobacterium breve Exopolysaccharide Blocks Dendritic Cell Maturation and Activation of CD4+ T Cells
    Authors: A Hickey, P Stamou, S Udayan, A Ramón-Vázq, M Esteban-To, F Bottacini, JA Woznicki, O Hughes, S Melgar, M Ventura, D Van Sinder, V Rossini, K Nally
    Frontiers in Microbiology, 2021-06-16;12(0):653587.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Ex Vivo-Induced Bone Marrow-Derived Myeloid Suppressor Cells Prevent Corneal Allograft Rejection in Mice
    Authors: J Zhu, T Inomata, K Fujimoto, K Uchida, K Fujio, K Nagino, M Miura, N Negishi, Y Okumura, Y Akasaki, K Hirosawa, M Kuwahara, A Eguchi, H Shokirova, A Yanagawa, A Midorikawa, A Murakami
    Investigative Ophthalmology & Visual Science, 2021-06-01;62(7):3.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation
    Authors: HC Hope, RJ Brownlie, CM Fife, L Steele, M Lorger, RJ Salmond
    JCI Insight, 2021-05-10;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Programmed Death-Ligand 2 Deficiency Exacerbates Experimental Autoimmune Myocarditis in Mice
    Authors: S Li, K Tajiri, N Murakoshi, D Xu, S Yonebayash, Y Okabe, Z Yuan, D Feng, K Inoue, K Aonuma, Y Shimoda, Z Song, H Mori, H Huang, K Aonuma, M Ieda
    International Journal of Molecular Sciences, 2021-01-31;22(3):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis
    Authors: M Aftabizade, YJ Li, Q Zhao, C Zhang, N Ambaye, J Song, T Nagao, C Lahtz, M Fakih, DK Ann, H Yu, A Herrmann
    JCI Insight, 2021-01-25;6(2):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Modifying bacterial flagellin to evade Nod-like Receptor CARD 4 recognition enhances protective immunity against Salmonella
    Authors: P Tourlomous, JA Wright, AS Bittante, LJ Hopkins, SJ Webster, OJ Bryant, P Mastroeni, DJ Maskell, CE Bryant
    Nat Microbiol, 2020-10-26;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. An Antibody Targeting ICOS Increases Intratumoral Cytotoxic to Regulatory T-cell Ratio and Induces Tumor Regression
    Authors: RCA Sainson, AK Thotakura, M Kosmac, G Borhis, N Parveen, R Kimber, J Carvalho, SJ Henderson, KL Pryke, T Okell, S O'Leary, S Ball, C Van Krinks, L Gamand, E Taggart, EJ Pring, H Ali, H Craig, VWY Wong, Q Liang, RJ Rowlands, M Lecointre, J Campbell, I Kirby, D Melvin, V Germaschew, E Oelmann, S Quaratino, M McCourt
    Cancer Immunology Research, 2020-09-30;8(12):1568-1582.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. WAS Promoter-Driven Lentiviral Vectors Mimic Closely the Lopsided WASP Expression during Megakaryocytic Differentiation
    Authors: P Muñoz, M Tristán-Ma, A Sánchez-Gi, G Santilli, A Galy, AJ Thrasher, F Martin
    Mol Ther Methods Clin Dev, 2020-09-16;19(0):220-235.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Aptamer targeted therapy potentiates immune checkpoint blockade in triple-negative breast cancer
    Authors: S Camorani, M Passariell, L Agnello, S Esposito, F Collina, M Cantile, M Di Bonito, IV Ulasov, M Fedele, A Zannetti, C De Lorenzo, L Cerchia
    J. Exp. Clin. Cancer Res., 2020-09-07;39(1):180.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Isolation of Two Novel Human Anti-CTLA-4 mAbs with Intriguing Biological Properties on Tumor and NK Cells
    Authors: M Passariell, C Vetrei, E Sasso, G Froechlich, C Gentile, AM D'Alise, N Zambrano, E Scarselli, A Nicosia, C De Lorenzo
    Cancers (Basel), 2020-08-06;12(8):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Immunomodulatory Effect of a Salvia plebeia R. Aqueous Extract in Forced Swimming Exercise-induced Mice
    Authors: J Shin, OK Kim, S Kim, D Bae, J Lee, J Park, W Jun
    Nutrients, 2020-07-28;12(8):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Combination therapy using human papillomavirus L1/E6/E7 genes and archaeosome: a nanovaccine confer immuneadjuvanting effects to fight cervical cancer
    Authors: H Karimi, H Soleimanja, A Abdoli, RS Banijamali
    Sci Rep, 2020-04-01;10(1):5787.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Acorus gramineusand and Euodia ruticarpa Steam Distilled Essential Oils Exert Anti-Inflammatory Effects Through Decreasing Th1/Th2 and Pro-/Anti-Inflammatory Cytokine Secretion Ratios In Vitro
    Authors: TH Yeh, JY Lin
    Biomolecules, 2020-02-19;10(2):.
    Species: Mouse
    Sample Types: Cell Lysates
  25. Antitumor and antioxidant effects of Clinacanthus nutans Lindau in 4?T1 tumor-bearing mice
    Authors: NMA Nik Abd Ra, MY Nurliyana, MNFNN Afiqah, MA Osman, M Hamid, MAM Lila
    BMC Complement Altern Med, 2019-11-29;19(1):340.
    Species: Mouse
    Sample Types: Serum
  26. Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics
    Authors: G Voisinne, K Kersse, K Chaoui, L Lu, J Chaix, L Zhang, M Goncalves, L Girard, Y Ounoughene, H Wang, O Burlet-Sch, H Luche, F Fiore, M Malissen, A Gonzalez d, Y Liang, R Roncagalli, B Malissen
    Nat. Immunol., 2019-10-07;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Nano spray dryer for vectorizing ?-galactosylceramide in polymeric nanoparticles: A single step process to enhance invariant Natural Killer T lymphocyte responses
    Authors: MB Gonzatti, MEP Sousa, AS Tunissi, RA Mortara, AM de Oliveir, NN Pereira Ce, AC Keller
    Int J Pharm, 2019-05-07;565(0):123-132.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Rapid synthesis and immunogenicity of mycobacterial (1?5)-?-d-arabinofuranan
    Authors: H Leelayuwap, S Ruchirawat, S Boonyaratt
    Carbohydr Polym, 2018-10-24;206(0):262-272.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. ATX-MS-1467 Induces Long-Term Tolerance to Myelin Basic Protein in (DR2?�?Ob1)F1 Mice by Induction of IL-10-Secreting iTregs
    Authors: ALS De Souza, S Rudin, R Chang, K Mitchell, T Crandall, S Huang, JK Choi, SL Okitsu, DL Graham, B Tomkinson, T Dellovade
    Neurol Ther, 2018-03-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. A Restricted Role for Fc?R in the Regulation of Adaptive Immunity
    Authors: MF Fransen, H Benonisson, WW van Maren, HS Sow, C Breukel, MM Linssen, JWC Claassens, C Brouwers, J van der Ka, M Camps, JW Kleinovink, KK Vonk, S van Heinin, N Klar, L van Beek, V van Harmel, L Daxinger, KS Nandakumar, R Holmdahl, C Coward, Q Lin, S Hirose, D Salvatori, T van Hall, C van Kooten, P Mastroeni, F Ossendorp, JS Verbeek
    J. Immunol., 2018-03-09;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Therapeutic effect of human adipose-derived stem cells and their secretome in experimental diabetic pain
    Authors: AT Brini, G Amodeo, LM Ferreira, A Milani, S Niada, G Moschetti, S Franchi, E Borsani, LF Rodella, AE Panerai, P Sacerdote
    Sci Rep, 2017-08-29;7(1):9904.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. Lamtor1 Is Critically Required for CD4(+) T Cell Proliferation and Regulatory T Cell Suppressive Function
    Authors: T Hosokawa, T Kimura, S Nada, T Okuno, D Ito, S Kang, S Nojima, K Yamashita, T Nakatani, Y Hayama, Y Kato, Y Kinehara, M Nishide, N Mikami, S Koyama, H Takamatsu, D Okuzaki, N Ohkura, S Sakaguchi, M Okada, A Kumanogoh
    J. Immunol., 2017-08-02;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  33. Inducing maternal inflammation promotes leptin production in offspring but does not improve allergic symptoms in a mouse model of allergic rhinitis
    Authors: A Imai, K Satoi, E Fujimoto, K Sato
    Heliyon, 2017-06-22;3(6):e00327.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. Synthetic Cannabinoid-Induced Immunosuppression Augments Cerebellar Dysfunction in Tetanus-Toxin Treated Mice
    Authors: J Yun, SM Gu, TH Lee, YJ Song, S Seong, YH Kim, HJ Cha, KM Han, J Shin, H Oh, K Jung, C Ahn, HK Park, HS Kim
    Biomol Ther (Seoul), 2017-05-01;25(3):266-271.
    Species: Mouse
    Sample Types: Tissue Homogenates
  35. Effects of gold nanoparticle-based vaccine size on lymph node delivery and cytotoxic T-lymphocyte responses
    Authors: S Kang, S Ahn, J Lee, JY Kim, M Choi, V Gujrati, H Kim, J Kim, EC Shin, S Jon
    J Control Release, 2017-04-18;256(0):56-67.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  36. Th17 cells are refractory to senescence and retain robust antitumor activity after long-term ex vivo expansion
    Authors: JS Bowers, MH Nelson, K Majchrzak, SR Bailey, B Rohrer, AD Kaiser, C Atkinson, L Gattinoni, CM Paulos
    JCI Insight, 2017-03-09;2(5):e90772.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  37. CD74 Deficiency Mitigates Systemic Lupus Erythematosus-like Autoimmunity and Pathological Findings in Mice
    Authors: Y Zhou, H Chen, L Liu, X Yu, GK Sukhova, M Yang, L Zhang, VC Kyttaris, GC Tsokos, IE Stillman, T Ichimura, JV Bonventre, P Libby, GP Shi
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Serum
  38. NK cells require antigen-specific memory CD4+ T cells to mediate superior effector functions during HSV-2 recall responses in vitro
    J. Leukoc. Biol, 2016-12-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Comparison of potential protection conferred by three immunization strategies (protein/protein, DNA/DNA, and DNA/protein) against Brucella infection using Omp2b in BALB/c Mice
    Authors: Saeid Bouzari
    Vet. Microbiol., 2016-11-05;197(0):47-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. Tuberculosis Susceptibility and Vaccine Protection Are Independently Controlled by Host Genotype
    MBio, 2016-09-20;7(5):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. Testosterone-Mediated Endocrine Function and TH1/TH2 Cytokine Balance after Prenatal Exposure to Perfluorooctane Sulfonate: By Sex Status
    Int J Mol Sci, 2016-09-12;17(9):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  42. Dendritic Cell-Like Cells Accumulate in Regenerating Murine Skeletal Muscle after Injury and Boost Adaptive Immune Responses Only upon a Microbial Challenge
    PLoS ONE, 2016-05-19;11(5):e0155870.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis
    PLoS ONE, 2016-05-09;11(5):e0154630.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  44. Immune Cell-Conditioned Media Suppress Prostate Cancer PC-3 Cell Growth Correlating With Decreased Proinflammatory/Anti-inflammatory Cytokine Ratios in the Media Using 5 Selected Crude Polysaccharides
    Integr Cancer Ther, 2016-04-29;15(4):NP13-NP25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  45. TARM1 Is a Novel Leukocyte Receptor Complex-Encoded ITAM Receptor That Costimulates Proinflammatory Cytokine Secretion by Macrophages and Neutrophils.
    Authors: Radjabova V, Mastroeni P, Skjodt K, Zaccone P, de Bono B, Goodall J, Chilvers E, Juss J, Jones D, Trowsdale J, Barrow A
    J Immunol, 2015-08-26;195(7):3149-59.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Cellular FLIP Inhibits Myeloid Cell Activation by Suppressing Selective Innate Signaling.
    Authors: Wu Y, Wu Y, Mo S, Hsiao H, He Y, Lai M
    J Immunol, 2015-08-03;195(6):2612-23.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  47. TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse.
    Authors: Na B, Kim H, Piragyte I, Oh H, Kwon M, Akber U, Lee H, Park D, Song W, Park Z, Im S, Rho M, Hyun Y, Kim M, Jun C
    J Cell Biol, 2015-04-13;209(1):143-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  48. Simultaneous and dose dependent melanoma cytotoxic and immune stimulatory activity of betulin.
    Authors: Pfarr K, Danciu C, Arlt O, Neske C, Dehelean C, Pfeilschifter J, Radeke H
    PLoS ONE, 2015-03-10;10(3):e0118802.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. The glycosylated Rv1860 protein of mycobacterium tuberculosis inhibits dendritic cell mediated TH1 and TH17 polarization of T cells and abrogates protective immunity conferred by BCG.
    Authors: Satchidanandam V, Kumar N, Jumani R, Challu V, Elangovan S, Khan N
    PLoS Pathog, 2014-06-12;10(6):e1004176.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  50. Dynamic motile T cells highly respond to the T cell stimulation via PI3K-Akt and NF-kappaB pathways.
    Authors: Kim H, Na B, Kwon M, Ko Y, Han W, Jun C
    PLoS ONE, 2013-03-26;8(3):e59793.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. Genetic deletion of the HIF-1alpha isoform I.1 in T cells enhances antibacterial immunity and improves survival in a murine peritonitis model.
    Authors: Georgiev P, Belikoff B, Hatfield S, Ohta A, Sitkovsky M, Lukashev D
    Eur J Immunol, 2013-01-31;43(3):655-66.
    Species: Mouse
    Sample Types: Serum
  52. Role of OGR1 in myeloid-derived cells in prostate cancer.
    Authors: Yan, L, Singh, L S, Zhang, L, Xu, Y
    Oncogene, 2012-12-10;33(2):157-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2011-10-24;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  54. Api6/AIM/Sp{alpha}/CD5L Overexpression in Alveolar Type II Epithelial Cells Induces Spontaneous Lung Adenocarcinoma.
    Authors: Li Y, Qu P, Wu L, Li B, Du H, Yan C
    Cancer Res., 2011-06-22;71(16):5488-99.
    Species: Mouse
    Sample Types: Serum
  55. Mucosal immunity in mice induced by orally administered transgenic rice.
    Authors: Zhang X, Yuan Z, Duan Q, Zhu H, Yu H, Wang Q
    Vaccine, 2009-01-13;27(10):1596-600.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. CD69+ CD4+ CD25- T cells, a new subset of regulatory T cells, suppress T cell proliferation through membrane-bound TGF-beta 1.
    Authors: Han Y, Guo Q, Zhang M, Chen Z, Cao X
    J. Immunol., 2009-01-01;182(1):111-20.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  57. Identification of cardiac troponin I sequence motifs leading to heart failure by induction of myocardial inflammation and fibrosis.
    Authors: Kaya Z, Goser S, Buss SJ, Leuschner F, Ottl R, Li J, Volkers M, Zittrich S, Pfitzer G, Rose NR, Katus HA
    Circulation, 2008-10-27;118(20):2063-72.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation.
    Authors: Lages CS, Suffia I, Velilla PA, Huang B, Warshaw G, Hildeman DA, Belkaid Y, Chougnet C
    J. Immunol., 2008-08-01;181(3):1835-48.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  59. Adenosine A2A receptor activation inhibits T helper 1 and T helper 2 cell development and effector function.
    Authors: Csoka B, Himer L, Selmeczy Z, Vizi ES, Pacher P, Ledent C, Deitch EA, Spolarics Z, Nemeth ZH, Hasko G
    FASEB J., 2008-07-14;22(10):3491-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  60. Amelioration of delayed-type hypersensitivity responses by IL-27 administration.
    Authors: Miyazaki Y, Shimanoe Y, Wang S, Yoshida H
    Biochem. Biophys. Res. Commun., 2008-06-20;373(3):397-402.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. Streptococcus equi antigens adsorbed onto surface modified poly-epsilon-caprolactone microspheres induce humoral and cellular specific immune responses.
    Authors: Florindo HF, Pandit S, Goncalves LM, Alpar HO, Almeida AJ
    Vaccine, 2008-06-17;26(33):4168-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  62. Effect of intestinal microbiota on the induction of regulatory CD25+ CD4+ T cells.
    Authors: Ishikawa H, Tanaka K, Maeda Y, Aiba Y, Hata A, Tsuji NM, Koga Y, Matsumoto T
    Clin. Exp. Immunol., 2008-05-05;153(1):127-35.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  63. Suppression of autoimmune inflammation of the central nervous system by interleukin 10 secreted by interleukin 27-stimulated T cells.
    Authors: Fitzgerald DC, Zhang GX, El-Behi M, Fonseca-Kelly Z, Li H, Yu S, Saris CJ, Gran B, Ciric B, Rostami A
    Nat. Immunol., 2007-11-11;8(12):1372-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  64. CD40L disruption enhances Abeta vaccine-mediated reduction of cerebral amyloidosis while minimizing cerebral amyloid angiopathy and inflammation.
    Authors: Obregon D, Hou H, Bai Y, Nikolic WV, Mori T, Luo D, Zeng J, Ehrhart J, Fernandez F, Morgan D, Giunta B, Town T, Tan J
    Neurobiol. Dis., 2007-10-16;29(2):336-53.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  65. Osteopontin regulates hindlimb-unloading-induced lymphoid organ atrophy and weight loss by modulating corticosteroid production.
    Authors: Wang KX, Shi Y, Denhardt DT
    Proc. Natl. Acad. Sci. U.S.A., 2007-09-04;104(37):14777-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  66. The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes.
    Authors: Perchonock CE, Pajerowski AG, Nguyen C, Shapiro MJ, Shapiro VS
    J. Immunol., 2007-08-01;179(3):1768-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  67. Lactoferrin enhanced efficacy of the BCG vaccine to generate host protective responses against challenge with virulent Mycobacterium tuberculosis.
    Authors: Hwang SA, Wilk KM, Budnicka M, Olsen M, Bangale YA, Hunter RL, Kruzel ML, Actor JK
    Vaccine, 2007-07-27;25(37):6730-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  68. CD8+ T cells are not required for vaccine-induced immunity against Leishmania amazonensis in IL-12/23P40(-/-) C57BL/6 mice.
    Authors: Hernandez Sanabria MX, Afonso LC, Golgher D, Tafuri WL, Vieira LQ
    Microbes Infect., 2007-05-18;9(9):1124-34.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  69. Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
    Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
    Immunity, 2007-04-12;26(4):445-59.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  70. Differing effects of clarithromycin and azithromycin on cytokine production by murine dendritic cells.
    Authors: Sugiyama K, Shirai R, Mukae H, Ishimoto H, Nagata T, Sakamoto N, Ishii H, Nakayama S, Yanagihara K, Mizuta Y, Kohno S
    Clin. Exp. Immunol., 2007-03-01;147(3):540-6.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  71. An alternative pathway of NF-kappaB activation results in maturation and T cell priming activity of dendritic cells overexpressing a mutated IkappaBalpha.
    Authors: Moore F, Buonocore S, Aksoy E, Ouled-Haddou N, Goriely S, Lazarova E, Paulart F, Heirman C, Vaeremans E, Thielemans K, Goldman M, Flamand V
    J. Immunol., 2007-02-01;178(3):1301-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  72. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX.
    Authors: Perchonock CE, Fernando MC, Quinn WJ, Nguyen CT, Sun J, Shapiro MJ, Shapiro VS
    Mol. Cell. Biol., 2006-08-01;26(16):6005-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  73. Transgenic expression of a CD83-immunoglobulin fusion protein impairs the development of immune-competent CD4-positive T cells.
    Authors: Luthje K, Cramer SO, Ehrlich S, Veit A, Steeg C, Fleischer B, Bonin A, Breloer M
    Eur. J. Immunol., 2006-08-01;36(8):2035-45.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  74. Exogenous IL-12 suppresses experimental autoimmune encephalomyelitis (EAE) by tuning IL-10 and IL-5 levels in an IFN-gamma-dependent way.
    Authors: Berghmans N, Dillen C, Heremans H
    J. Neuroimmunol., 2006-06-09;176(1):63-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  75. Effects of dexamethazone on LPS-induced activationand migration of mouse dendritic cells revealed by a genome-wide transcriptional analysis.
    Authors: Vizzardelli C, Pavelka N, Luchini A, Zanoni I, Bendickson L, Pelizzola M, Beretta O, Foti M, Granucci F, Nilsen-Hamilton M, Ricciardi-Castagnoli P
    Eur. J. Immunol., 2006-06-01;36(6):1504-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  76. Induction of CTLA-4-mediated anergy contributes to persistent colonization in the murine model of gastric Helicobacter pylori infection.
    Authors: Anderson KM, Czinn SJ, Redline RW, Blanchard TG
    J. Immunol., 2006-05-01;176(9):5306-13.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  77. The effects of dehydroepiandrosterone (DHEA) on in vitro spleen cell proliferation and cytokine production.
    Authors: Powell JM, Sonnenfeld G
    J. Interferon Cytokine Res., 2006-01-01;26(1):34-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005-03-27;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  79. Comparative immunomodulatory properties of a chitosan-MDP adjuvant combination following intranasal or intramuscular immunisation.
    Authors: Moschos SA, Bramwell VW, Somavarapu S, Alpar HO
    Vaccine, 2005-03-14;23(16):1923-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  80. Leukaemia inhibitory factor (LIF) is functionally linked to axotrophin and both LIF and axotrophin are linked to regulatory immune tolerance.
    Authors: Metcalfe SM, Muthukumarana PA, Thompson HL, Haendel MA, Lyons GE
    FEBS Lett., 2005-01-31;579(3):609-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Subcellular fractions of Brucella ovis distinctively induce the production of interleukin-2, interleukin-4, and interferon-gamma in mice.
    Authors: Salas-Tellez E, Nunez del Arco A, Tenorio V, Diaz-Aparicio E, de la Garza M, Suarez-Guemes F
    Can. J. Vet. Res., 2005-01-01;69(1):53-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  82. Comparison of immunogenicities of recombinant Plasmodium vivax merozoite surface protein 1 19- and 42-kiloDalton fragments expressed in Escherichia coli.
    Authors: Sachdeva S, Ahmad G, Malhotra P, Mukherjee P, Chauhan VS
    Infect. Immun., 2004-10-01;72(10):5775-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  83. Active hexose correlated compound enhances the immune function of mice in the hindlimb-unloading model of spaceflight conditions.
    Authors: Aviles H, Belay T, Vance M, Sun B, Sonnenfeld G
    J. Appl. Physiol., 2004-06-11;97(4):1437-44.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  84. Intracellular thiols contribute to Th2 function via a positive role in IL-4 production.
    Authors: Monick MM, Samavati L, Butler NS, Mohning M, Powers LS, Yarovinsky T, Spitz DR, Hunninghake GW
    J. Immunol., 2003-11-15;171(10):5107-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  85. Transcutaneous immunization with cholera toxin B subunit adjuvant suppresses IgE antibody responses via selective induction of Th1 immune responses.
    Authors: Anjuere F, George-Chandy A, Audant F, Rousseau D, Holmgren J, Czerkinsky C
    J. Immunol., 2003-02-01;170(3):1586-92.
    Species: Mouse
    Sample Types:

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Mouse IL-2 DuoSet ELISA
By Anonymous on 11/02/2017
Sample Tested: Cell culture supernatant
Mouse IL-2 DuoSet ELISA, 15 Plate
By Anonymous on 10/26/2015
Sample Tested: Lymphocytes (ex vivo)

Specificity: Specific
Sensitivity: Sensitive
Buffer: 1% BSA in PBS, pH 7.2-7.4, filtered
Dilution: 1/180

Mouse IL-2 DuoSet ELISA, 15 Plate DY402