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Reagent Diluent Concentrate 2

Catalog #: DY995
39 Citations 28 Reviews Datasheet / COA / SDS
10X, 5 x 21 mL
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DY995
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Reagent Diluent Concentrate 2
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Product Details
Citations (39)
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Supplemental Products
Reviews (28)
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Reagent Diluent Concentrate 2 Summary

INTENDED USE & DESCRIPTION

Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.

REAGENT PREPARATION

For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information. 

Specifications

Source
N/A
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

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Product Datasheet
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Citations for Reagent Diluent Concentrate 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Chronic Glaucoma Induced in Rats by a Single Injection of Fibronectin-Loaded PLGA Microspheres: IOP-Dependent and IOP-Independent Neurodegeneration
    Authors: Munuera, I;Aragon-Navas, A;Villacampa, P;Gonzalez-Cela, MA;Subías, M;Pablo, LE;Garcia-Feijoo, J;Herrero-Vanrell, R;Garcia-Martin, E;Bravo-Osuna, I;Rodrigo, MJ;
    International journal of molecular sciences  2023-12-19
  2. Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization
    Authors: Kennedy, MS;Zhang, M;DeLeon, O;Bissell, J;Trigodet, F;Lolans, K;Temelkova, S;Carroll, KT;Fiebig, A;Deutschbauer, A;Sidebottom, AM;Lake, J;Henry, C;Rice, PA;Bergelson, J;Chang, EB;
    Cell reports  2023-08-29
  3. Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells
    Authors: Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H;
    Cells  2023-08-19
  4. Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
    Authors: MJ Rodrigo, I Bravo-Osun, M Subias, A Montolío, J Cegoñino, T Martinez-R, S Mendez-Mar, A Aragón-Nav, D Garcia-Her, LE Pablo, R Herrero-Va, AP Del Paloma, E Garcia-Mar
    Scientific Reports, 2022-11-30;12(1):20622.  2022-11-30
  5. Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
    Authors: H Kim, B Jang, YJ Kim, JC Choi
    International Journal of Molecular Sciences, 2022-09-11;23(18):.  2022-09-11
  6. Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
    Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
    Nature, 2021-11-03;0(0):.  2021-11-03
  7. Topological isolation of developmental regulators in mammalian genomes
    Authors: HJ Wu, A Landshamme, EK Stamenova, A Bolondi, H Kretzmer, A Meissner, F Michor
    Nature Communications, 2021-08-12;12(1):4897.  2021-08-12
  8. The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells
    Authors: F Zilli, P Marques Ra, P Auf der Ma, C Jehanno, A Sethi, MM Coissieux, T Eichlisber, L Sauteur, A Rouchon, L Bonapace, J Pinto Cout, R Rad, MR Jensen, A Banfi, MB Stadler, M Bentires-A
    Embo Molecular Medicine, 2021-03-10;0(0):e13162.  2021-03-10
  9. Detection of Plasmodium falciparum malaria in 1�h using a simplified enzyme-linked immunosorbent assay
    Authors: E de la Sern, K Arias-Alpí, LN Borgheti-C, A Sanchez-Ca, E Sulleiro, F Zarzuela, P Bosch-Nico, F Salvador, I Molina, M Ramírez, X Fernàndez-, A Sánchez-Mo, E Baldrich
    Analytica chimica acta, 2021-01-30;1152(0):338254.  2021-01-30
  10. Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
    Authors: L Kang, S Smith, C Wang
    Journal of colloid and interface science, 2020-12-24;588(0):101-109.  2020-12-24
  11. Differentially expressed circular RNAs in a murine asthma model
    Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
    Molecular Medicine Reports, 2020-10-20;22(6):5412-5422.  2020-10-20
  12. bFGF blockade reduces intraplaque angiogenesis and macrophage infiltration in atherosclerotic vein graft lesions in ApoE3*Leiden mice
    Authors: L Parma, HAB Peters, TJ Sluiter, KH Simons, P Lazzari, MR de Vries, PHA Quax
    Sci Rep, 2020-09-29;10(1):15968.  2020-09-29
  13. Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis
    Authors: J Vullings, JP Vago, CEJ Waterborg, RM Thurlings, MI Koenders, PLEM van Lent, PM van der Kr, FA Amaral, FAJ van de Loo
    J Immunol Res, 2020-09-11;2020(0):9690832.  2020-09-11
  14. BMP9 signaling promotes the normalization of tumor blood vessels
    Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
    Oncogene, 2020-02-10;0(0):.  2020-02-10
  15. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019-08-01;14(0):6117-6131.  2019-08-01
  16. Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
    Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
    J Clin Med, 2019-07-15;8(7):.  2019-07-15
  17. Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
    Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
    J Immunol Res, 2019-04-11;2019(0):1845128.  2019-04-11
  18. Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
    Cell Transplant, 2018-09-25;0(0):9636897187922.  2018-09-25
  19. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018-07-09;8(1):10328.  2018-07-09
  20. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018-02-01;2018(0):8286067.  2018-02-01
  21. Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
    Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
    Planta, 2018-01-08;0(0):.  2018-01-08
  22. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017-05-18;2(10):.  2017-05-18
  23. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
    Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
    Oncol Lett, 2017-04-21;13(6):4995-5001.  2017-04-21
  24. Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
    Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
    Oncol Lett, 2017-03-13;13(5):3269-3276.  2017-03-13
  25. Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains.
    Authors: Harlan S, Ostroski R, Coskun T, Yantis L, Breyer M, Heuer J
    Am J Physiol Regul Integr Comp Physiol, 2015-06-24;309(5):R467-74.  2015-06-24
  26. Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics.
    Authors: Wildsmith K, Schauer S, Smith A, Arnott D, Zhu Y, Haznedar J, Kaur S, Mathews W, Honigberg L
    Mol Neurodegener, 2014-06-06;9(0):22.  2014-06-06
  27. Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
    Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
    Nat Commun, 2014-04-30;5(0):3779.  2014-04-30
  28. Oral and subcutaneous therapy of canine atopic dermatitis with recombinant feline interferon omega.
    Authors: Litzlbauer P, Weber K, Mueller R
    Cytokine, 2014-01-14;66(1):54-9.  2014-01-14
  29. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012-06-20;7(6):e39036.  2012-06-20
  30. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011-10-21;187(11):5745-55.  2011-10-21
  31. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011-04-19;19(10):1793-801.  2011-04-19
  32. Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
    Authors: Derycke MS, Pambuccian SE, Gilks CB
    Am. J. Clin. Pathol., 2010-11-01;134(5):835-45.  2010-11-01
  33. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010-07-01;85(7):487-93.  2010-07-01
  34. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010-02-16;5(2):e9246.  2010-02-16
  35. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2009-12-10;33(3):664-9.  2009-12-10
  36. A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
    Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
    Clin. Exp. Allergy, 2008-02-25;38(4):594-601.  2008-02-25
  37. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008-02-08;4(2):e41.  2008-02-08
  38. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007-08-01;67(15):7458-66.  2007-08-01
  39. Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
    Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
    J. Neurochem., 2006-02-08;96(6):1560-71.  2006-02-08

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Reviews for Reagent Diluent Concentrate 2

Average Rating: 4.9 (Based on 28 Reviews)

5 Star
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4 Star
7.14%
3 Star
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Reagent Diluent Concentrate 2
By Anonymous on 07/01/2024
Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 05/21/2024

worked well with DY2780.

Reagent Diluent Concentrate 2
By Anonymous on 05/23/2022

Great for use in ELISAs. Consistent product and convenient to use.

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 09/23/2021
Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 01/31/2021

Effective blocking reagent for FACs binding assays. The multiple vials make them easy for one time use on days of assays without having to worry about contaminating a large stock.

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Hon Wai Koon on 12/01/2020
Reagent Diluent Concentrate 2
By Anonymous on 11/22/2020
Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 09/14/2020

Always produces great results with every duoset I have used. For me, this is usually the only additional reagent I need outside the Ancillary Reagent Kit 2 that I usually purchase

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 08/17/2020
Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 01/17/2020
Reagent Diluent Concentrate 2
By Anonymous on 01/10/2020
Reagent Diluent Concentrate 2
By Tahmineh Safaie on 12/23/2019

I have been using the this for several ELISA assays and has been working with no problem

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 09/23/2019

excellent blocker, we used 7 kits so far. Works well for serum ELISA's

Reagent Diluent Concentrate 2
By Anonymous on 03/28/2019

I use this as my blocking buffer and antibody diluent buffer in all my ELISAs. I like that it comes as a concentrate

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Marry Tahani on 11/20/2018

Gives really good linearity.

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 08/17/2018
Reagent Diluent Concentrate 2
By Anonymous on 07/17/2018

Great blocker

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 06/15/2018
Reagent Diluent Concentrate 2
By Anonymous on 04/30/2018
Reagent Diluent Concentrate 2
By Anonymous on 04/11/2018
Reagent Diluent Concentrate 2
By Leslie Priddy on 04/03/2018
Reagent Diluent Concentrate 2
By Anonymous on 02/06/2018
Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018

I use RD to make standard curves

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/19/2017

Used the reagent diluent for assay and it works perfect has very little to no background

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL) DY995
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Shubhanchi Nigam on 07/14/2017
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL) DY995
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/06/2017
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 03/14/2017

Used for Duoset ELISA