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Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit

Catalog #: MSST00
13 Citations 2 Reviews Datasheet / COA / SDS
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MSST00
Mouse/Rat SOST/Sclerostin ELISA Standard Curve
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Product Details
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Citations (13)
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Supplemental Products
Reviews (2)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
4.17 pg/mL
Assay Range
15.6 - 1,000 pg/mL
Specificity
Natural and recombinant mouse and rat SOST
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat SOST immunoassay is a 4.5 hour solid-phase ELISA designed to measure SOST in mouse or rat cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse SOST and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SOST. Results obtained using natural mouse or rat SOST showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SOST.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 57.6 108 345 54.4 101 347
Standard Deviation 4.15 6 18.7 4.35 6.23 19.4
CV% 7.2 5.6 5.4 8 6.2 5.6

Recovery

The recovery of SOST spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 101 92-112
Mouse EDTA Plasma (n=4) 98 82-113
Mouse Heparin Plasma (n=4) 102 88-115
Mouse Serum (n=4) 95 86-113
Rat EDTA Plasma (n=2) 107 101-118
Rat Heparin Plasma (n=2) 96 82-111
Rat Serum (n=4) 99 85-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SOST in each matrix were diluted with Calibrator Diluent and assayed.
Mouse/Rat SOST/Sclerostin ELISA Linearity

Scientific Data

N/A Mouse/Rat SOST/Sclerostin ELISA Standard Curve View Larger

Mouse/Rat SOST/Sclerostin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SOST/Sclerostin

SOST, also known as Sclerostin, is a Cerberus/DAN family BMP antagonist and is an important regulator of bone homeostasis. Inactivating mutations in the SOST gene can cause sclerosteosis and van Buchem disease. SOST is expressed by terminally differentiated cells embedded in mineralized matrix including osteocytes, hypertrophic and prehypertrophic chondrocytes, and tooth cementocytes. Its expression is induced by BMP-2, -4, and -6 and is inhibited by parathyroid hormone (PTH). Its downregulation in osteocytes by physical loading of bone contributes to the mechanical sensor function of osteocytes and the subsequent increase in bone growth. SOST binds to BMP-2, -4, -5, -6, and -7 and inhibits the osteogenic differentiation induced by these BMPs. It inhibits canonical Wnt signaling by binding to LRP-5 and LRP-6 and inhibiting their association with Frizzled receptors. SOST reduces the proliferation of mesenchymal stem cells (MSC) and induces MSC apoptosis. Circulating levels of SOST are elevated in pathologies with bone involvement including low bone mineral density, Paget’s disease, multiple myeloma, and prostate cancer with bone metastases. It is also elevated in advanced chronic kidney disease, alcoholic liver disease, type 2 diabetes, and patients with increased abdominal and gynoid fat.

Entrez Gene IDs:
50964 (Human); 74499 (Mouse)
Alternate Names:
sclerostin; SOST; VBCHsclerosteosis
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, control, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice
    Authors: N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou
    Frontiers in Physiology, 2023-01-04;13(0):1061715.
    Species: Mouse
    Sample Types: Serum
  2. Osteocytic cells exposed to titanium particles increase sclerostin expression and inhibit osteoblastic cell differentiation mostly via direct cell-to-cell contact
    Authors: H Chai, ZH Zhang, JY Fang, C She, C Geng, W Xu
    Journal of Cellular and Molecular Medicine, 2022-06-28;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Sp7 Transgenic Mice with a Markedly Impaired Lacunocanalicular Network Induced Sost and Reduced Bone Mass by Unloading
    Authors: T Moriishi, T Ito, R Fukuyama, X Qin, H Komori, H Kaneko, Y Matsuo, N Yoshida, T Komori
    International Journal of Molecular Sciences, 2022-03-15;23(6):.
    Species: Mouse
    Sample Types: Serum
  4. Subcutaneous adipose tissue sclerostin is reduced and Wnt signaling is enhanced following 4-weeks of sprint interval training in young men with obesity
    Authors: N Kurgan, H Islam, JBL Matusiak, BJ Baranowski, J Stoikos, VA Fajardo, REK MacPherson, BJ Gurd, P Klentrou
    Physiological Reports, 2022-03-01;10(6):e15232.
    Species: Human
    Sample Types: Serum
  5. Novel Aptamer-Based Small-Molecule Drug Screening Assay to Identify Potential Sclerostin Inhibitors against Osteoporosis
    Authors: CC Lee, CM Hung, CH Chen, YC Hsu, YP Huang, TB Huang, MJ Lee
    International Journal of Molecular Sciences, 2021-08-02;22(15):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Sclerostin expression in trabecular bone is downregulated by osteoclasts
    Authors: M Koide, T Yamashita, K Murakami, S Uehara, K Nakamura, M Nakamura, M Matsushita, T Ara, H Yasuda, JM Penninger, N Takahashi, N Udagawa, Y Kobayashi
    Sci Rep, 2020-08-13;10(1):13751.
    Species: Mouse
    Sample Types: Bone Marrow Fluid, Whole Tissue
  7. Influences of donor and host age on human muscle-derived stem cell-mediated bone regeneration
    Authors: X Gao, A Lu, Y Tang, J Schneppend, AB Liebowitz, AC Scibetta, ER Morris, H Cheng, C Huard, S Amra, B Wang, MA Hall, WR Lowe, J Huard
    Stem Cell Res Ther, 2018-11-21;9(1):316.
    Species: Mouse
    Sample Types: Serum
  8. Osteoblastic Lrp4 promotes osteoclastogenesis by regulating ATP release and adenosine-A2AR signaling
    Authors: L Xiong, JU Jung, HH Guo, JX Pan, XD Sun, L Mei, WC Xiong
    J. Cell Biol, 2017-02-13;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Acute Phosphate Restriction Impairs Bone Formation and Increases Marrow Adipose Tissue in Growing Mice
    Authors: Frank C Ko
    J Bone Miner Res, 2016-11-07;0(0):.
    Species: Mouse
    Sample Types: Serum
  10. Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by different mechanisms in cancellous and cortical bone
    Authors: K Ogura, T Iimura, Y Makino, A Sugie-Oya, A Takakura, R Takao-Kawa, T Ishizuya, K Moriyama, A Yamaguchi
    Bone Rep, 2016-01-16;5(0):7-14.
    Species: Rat
    Sample Types: Serum
  11. Sclerostin inhibition prevents spinal cord injury-induced cancellous bone loss.
    Authors: Beggs L, Ye F, Ghosh P, Beck D, Conover C, Balaez A, Miller J, Phillips E, Zheng N, Williams A, Aguirre J, Wronski T, Bose P, Borst S, Yarrow J
    J Bone Miner Res, 2015-04-01;30(4):681-9.
    Species: Rat
    Sample Types: Serum
  12. Androgens inhibit the osteogenic response to mechanical loading in adult male mice.
    Authors: Sinnesael M, Laurent M, Jardi F, Dubois V, Deboel L, Delisser P, Behets G, D'Haese P, Carmeliet G, Claessens F, Vanderschueren D
    Endocrinology, 2015-02-05;156(4):1343-53.
    Species: Mouse
    Sample Types: Serum
  13. Role of TGF-beta in a mouse model of high turnover renal osteodystrophy.
    Authors: Liu S, Song W, Boulanger J, Tang W, Sabbagh Y, Kelley B, Gotschall R, Ryan S, Phillips L, Malley K, Cao X, Xia T, Zhen G, Cao X, Ling H, Dechow P, Bellido T, Ledbetter S, Schiavi S
    J Bone Miner Res, 2014-01-01;29(5):1141-57.
    Species: Mouse
    Sample Types: Serum

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Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit
By Anonymous on 04/10/2018
Sample Tested: activated mouse CD8 T cell
Mouse/Rat SOST/Sclerostin Quantikine ELISA Kit
By Anonymous on 04/05/2018
Sample Tested: Serum