Mouse/Rat Thrombopoietin/Tpo Antibody

Catalog #: AF-488-NA
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-488-NA
AF-488-SP

Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Mouse Thrombopoietin/Tpo Antibody.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Thrombopoietin/Tpo Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat Thrombopoietin/Tpo in direct ELISAs. Neutralizes the biological activity of recombinant mouse Thrombopoietin/Tpo. It will also neutralize the activity of recombinant human (rh) Thrombopoietin/Tpo, although 25 times the amount of Ig is required. In direct ELISAs less than 15% cross‑reactivity with rhTpo is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Thrombopoietin/Tpo and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Thrombopoietin/Tpo

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO)

Neutralization

Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 0.1‑0.3 µg/mL in the presence of 3 ng/mL Recombinant Mouse Thrombopoietin/Tpo.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Mouse Thrombopoietin/Tpo Antibody. Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Thrombopoietin/Tpo (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA). The ND₅₀ is typically 0.1-0.3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1‑3). The 356 amino acid (aa) mouse Tpo precursor is cleaved to yield the 335 aa mature protein. Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80‑85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of mouse Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10 - 13). It is cleaved by platelet-derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling (15, 16).

References

Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
Li, J. et al. (1999) Br. J. Haematol. 106:345.
Bartley, T.D. et al. (1994) Cell 77:1117.
de Sauvage, F.J. et al. (1994) Nature 369:533.
Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
Kaushansky, K. et al. (1994) Nature 369:568.
Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
Broudy, V.C. et al. (1995) Blood 85:1719.
Lok, S.I. et al. (1994) Nature 369:565.
Chen, J. et al. (1995) Blood 86:4054.
Oda, A. et al. (1996) Blood 87:4664.
Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
Samoylenko, A. et al. (2008) Cell. Signal. 20:154.

Entrez Gene IDs

7066 (Human); 21832 (Mouse); 81811 (Rat)

Alternate Names

Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MKCSF; MK-CSF; ML; MPL ligand; MPLLG; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THCYT1; THPO; thrombopoietin nirs variant 1; Thrombopoietin; Tpo; TPOMKCSF

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Citations for Mouse/Rat Thrombopoietin/Tpo Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Adipocyte Fatty Acid Transfer Supports Megakaryocyte Maturation
Authors: C Valet, A Batut, A Vauclard, A Dortignac, M Bellio, B Payrastre, P Valet, S Severin
Cell Rep, 2020-07-07;32(1):107875.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Thrombopoietin contributes to the formation and the maintenance of hematopoietic progenitor-containing cell clusters in the aorta-gonad-mesonephros region
Authors: K Harada, I Nobuhisa, M Anani, K Saito, T Taga
Cytokine, 2017-02-21;95(0):35-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Authors: Shih CC, Hu MC, Hu J
Blood, 2000-03-15;95(6):1957-66.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization