Mouse TMEFF1/Tomoregulin-1 Biotinylated Antibody

Catalog #: BAF1995 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF1995

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TMEFF1/Tomoregulin-1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects TMEFF1/Tomoregulin‑1 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human (rh) TMEFF1 is observed and less than 2% cross-reactivity with rhTMEFF2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TMEFF1/Tomoregulin‑1
Ser37-Thr320
Accession # Q6PFE7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TMEFF1/Tomoregulin‑1

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse spinal cord

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEFF1/Tomoregulin-1

TMEFF1, also known as tomoregulin 1 is a type I transmembrane glycoprotein that contains two follistatin modules and an EGF domain in its extracellular domain, a transmembrane domain and a short cytoplasmic tail. The extracellular domain can be released as a soluble protein. TMEFF1 selectively regulates nodal but not activin signaling through direct binding to the nodal co-receptor, Cripto.

Long Name

Transmembrane Protein with EFG-like and two Follistatin-like Domains

Entrez Gene IDs

8577 (Human); 230157 (Mouse)

Alternate Names

C9orf2; H7365member 1; TMEFF1; Tomoregulin1; Tomoregulin-1; TR-1; Transmembrane protein with EGF-like and one follistatin-like domain; transmembrane protein with EGF-like and two follistatin-like domains 1