Angiopoietin-like proteins (ANGPTL1-8) are secreted glycoproteins that are structurally related to Angiopoietins. They play roles in angiogenesis, lipid metabolism, hematopoietic stem cell expansion, inflammation, and cancer biology. ANGPTLs all contain an N-terminal coiled-coil domain which mediates oligomerization, and a C-terminal fibrinogen-like domain. ANGPTL8 is the only exception and doesn’t have the C-terminal domain. Unlike angiopoietins, which are known to signal through the tyrosine kinases Tie1 or Tie 2, Angiopoietin-like proteins have long been considered orphan ligands.
Recently, ANGPTL1, 2, 5, and 7 were shown to bind to human leukocyte immunoglobulin like receptor B2 (LILRB2), resulting in enhancement of human hematopoietic stem cell proliferation and expansion1. R&D Systems scientists have also shown that binding to LILR receptors is more widespread.
| Full Length | |
| Recombinant Human ANGPTL2 Protein | 9746-AN (New) |
| Recombinant Human ANGPTL3 Protein | 3829-AN |
| Recombinant Mouse ANGPTL3 Protein | 136-AN |
| Recombinant Human ANGPTL4 Protein | 4487-AN |
| Recombinant Mouse ANGPTL4 Protein | 4880-AN |
| Recombinant Human ANGPTL5 Protein | 6675-AN |
| Recombinant Human ANGPTL6 Protein | 8466-AN |
| Recombinant Human ANGPTL7 Protein | 914-AN |
| Recombinant Mouse ANGPTL7 Protein | 4960-AN |
| N-terminal Domain Only | |
| Recombinant Human ANGPTL3 Protein | 4954-AN |
| Recombinant Mouse ANGPTL3 Protein | 8344-AN |
| Recombinant Human ANGPTL4 Protein | 8249-AN |
| C-terminal Domain Only | |
| Recombinant Human ANGPTL2 Protein | 9795-AN |
| Recombinant Mouse ANGPTL2 Protein | 9796-AN |
| Recombinant Human ANGPTL4 Protein | 3485-AN |
| Recombinant Mouse ANGPTL4 Protein | 9797-AN |
| Biotinylated Protein Protein | |
| Recombinant Human ANGPTL4 Protein, Full Length | BT4487 |
| Recombinant Human ANGPTL7 Protein, Full Length | BT914 |
| Recombinant Mouse ANGPTL3 Protein, N-ter | BT8344 |
| Recombinant Human ANGPTL4 Protein, N-ter | BT8249 |
Human ANGPTL2 Binds the LILRB2 Receptor. An ELISA binding assay was used to show that Recombinant Human ANGPLT2 (Catalog # 9746-AN) binds to Recombinant Human LILRB2 (Catalog # 2078-T4). The ED50 for this interaction falls within 0.02-0.12 µg/mL.
Recombinant Human ANGPTL2 (Catalog # 9746-AN) Induces Proliferation in Primary Rat Liver Mononuclear Cells. ANGPTL2 enhances proliferation of E16 rat liver mononuclear cells grown, in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/TPO (Catalog # 488-TO), and Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL).
Human ANGPLT2 C-terminal Fragment Binds the LILRB2 Receptor. An ELISA binding assay was used to show that Recombinant Human ANGPLT2 C-terminal Fragment (Catalog # 9795-AN) binds to Recombinant Human LILRB2 (Catalog # 2078-T4). The ED50 for this interaction falls within 4-24 ng/mL.
Mouse ANGPLT2 C-terminal Fragmment Binds the LILRB2 Receptor. An ELISA binding assay was used to show that Recombinant Mouse ANGPLT2 C-terminal Fragment (Catalog # 9796-AN) binds to Recombinant Human LILRB2 (Catalog # 2078-T4). The ED50 for this interaction falls within 4-24 ng/mL.
Mouse ANGPLT4 C-terminal Fragmment Binds the LILRB2 Receptor. An ELISA binding assay was used to show that Recombinant Mouse ANGPLT4 C-terminal Fragment (Catalog # 9797-AN) binds to Recombinant Human LILRB2 (Catalog # 2078-T4). The ED50 for this interaction falls within 0.09-0.54 µg/mL.
References
- Zheng, J. et al. (2012) Inhibitory receptors bind ANGPTLs and support blood stem cells and leukaemia development. Nature 485:656.