Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2, CF

Catalog #: 9796-AN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9796-AN-050

Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2 Binding Activity

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All Angiopoietin-like Protein 2/ANGPTL2 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Angiopoietin‑like Protein 2/ANGPTL2 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) that produces 50% of the optimal binding response is 12-96 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 2/ANGPTL2 protein

Mouse ANGPTL2 (Ala260-His493) Accession # AAD55358	HP	GGGSGGGSGGGS	HHHHHH
N-terminus			C-terminus

Accession #

AAD55358

N-terminal Sequence
Analysis

Ala260

Predicted Molecular Mass

29 kDa

SDS-PAGE

28-39 kDa, reducing conditions

Product Datasheets

9796-AN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9796-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9796-AN) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) binds with an ED₅₀ of 12-96 ng/mL.

Reconstitution Calculator

Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2 is a secreted 56 kDa glycoprotein that contains a N-terminal coiled coil domain and a C-terminal fibrinogen like domain (1). ANGPTL2 is one of the seven members of the Angiopoietin Like family proteins, which are structurally similar to angiopoietins (2). Within mature mouse ANGPTL2, amino acid 260-493 contains the C-terminal fibrinogen like domain, and this domain shares 99% and 100% sequence identity with human and rat homologs, respectively. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6-7). ANGPTL2 also function as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled coil domain (8). Receptor of ANGPTL2 is recently identified as LILRB2, and both the coiled coil domain and fibrinogen like domain are required for optimal binding (9).

References