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Proteome Profiler Human XL Cytokine Array Kit

Catalog #: ARY022B
184 Citations 20 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 105 different cytokine antibodies
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ARY022B
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The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
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Citations (184)
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Reviews (20)
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Proteome Profiler Human XL Cytokine Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.

Key Benefits

  • Detects 105 human cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.
 

Principle of the Array

The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
Adiponectin/Acrp30 IFN-gamma CCL2/MCP-1
Angiogenin IGFBP-2 CCL7/MCP-3
Angiopoietin-1 IGFBP-3 M-CSF
Angiopoietin-2 IL-1 alpha/IL-1F1 MIF
Apolipoprotein A1 IL-1 beta/IL-1F2 CXCL9/MIG
BAFF/BLyS/TNFSF13B IL-1ra/IL-1F3 CCL3/CCL4 MIP-1 alpha/beta
BDNF IL-2 CCL20/MIP-3 alpha
CD14 IL-3 CCL19/MIP-3 beta
CD30 IL-4 MMP-9
CD31/PECAM-1 IL-5 Myeloperoxidase
CD40 Ligand/TNFSF5 IL-6 Osteopontin (OPN)
Chitinase 3-like IL-8 PDGF-AA
Complement Component C5/C5a IL-10 PDGF-AB/BB
Complement Factor D IL-11 Pentraxin 3/TSF-14
C-Reactive Protein/CRP IL-12 p70 CXCL4/PF4
Cripto-1 IL-13 RAGE
Cystatin C IL-15 CCL5/RANTES
Dkk-1 IL-16 RBP4
DPPIV/CD26 IL-17A Relaxin-2
EGF IL-18 BPa Resistin
CXCL5/ENA-78 IL-19 CXCL12/SDF-1 alpha
Endoglin/CD105 IL-22 Serpin E1/PAI-1
EMMPRIN IL-23 SHBG
Fas Ligand IL-24 ST2/IL1 R4
FGF basic IL-27 CCL17/TARC
KGF/FGF-7 IL-31 TFF3
FGF-19 IL-32 alpha/beta/gamma TfR
Flt-3 Ligand IL-33 TGF-alpha
G-CSF IL-34 Thrombospondin-1
GDF-15 CXCL10/IP-10 TIM-1
GM-CSF CXCL11/I-TAC TNF-alpha
CXCL1/GRO alpha Kallikrein 3/PSA uPAR
Growth Hormone (GH) Leptin VCAM-1
HGF LIF VEGF
ICAM-1/CD54 Lipocalin-2/NGAL Vitamin D BP

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

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Scientific Data

N/A The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. View Larger

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

N/A The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. View Larger

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

N/A The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. View Larger

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

Citations for Proteome Profiler Human XL Cytokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

184 Citations: Showing 1 - 10
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  1. Characteristics of the synovial microenvironment and synovial mesenchymal stem cells with hip osteoarthritis of different bone morphologies
    Authors: Yang, Y;Koga, H;Nakagawa, Y;Nakamura, T;Katagiri, H;Takada, R;Katakura, M;Tsuji, K;Sekiya, I;Miyatake, K;
    Arthritis research & therapy  2024-01-10
  2. Innate Immune Activation and Mitochondrial ROS Invoke Persistent Cardiac Conduction System Dysfunction after COVID-19
    Authors: Ashok, D;Liu, T;Criscione, J;Prakash, M;Kim, B;Chow, J;Craney, M;Papanicolaou, KN;Sidor, A;Brian Foster, D;Pekosz, A;Villano, J;Kim, DH;O'Rourke, B;
    bioRxiv : the preprint server for biology  2024-01-08
  3. Distinguishing the impact of distinct obstructive sleep apnea syndrome (OSAS) and obesity related factors on human monocyte subsets
    Authors: Pries, R;Kosyna, FK;Depping, R;Plötze-Martin, K;Lange, C;Meyhöfer, S;Meyhöfer, SM;Marquardt, JU;Bruchhage, KL;Steffen, A;
    Scientific reports  2024-01-03
  4. Overexpression of Growth Differentiation Factor 15 in Glioblastoma Stem Cells Promotes Their Radioresistance
    Authors: Bentaberry-Rosa, A;Nicaise, Y;Delmas, C;Gouazé-Andersson, V;Cohen-Jonathan-Moyal, E;Seva, C;
    Cancers  2023-12-20
  5. Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating features of native capillary network
    Authors: Sharma, D;Sharma, A;Hu, L;Chen, TA;Voon, S;Bayless, KJ;Goldman, J;Walsh, AJ;Zhao, F;
    Bioactive materials  2023-12-01
  6. Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells
    Authors: Chrisochoidou, Y;Roy, R;Farahmand, P;Gonzalez, G;Doig, J;Krasny, L;Rimmer, EF;Willis, AE;MacFarlane, M;Huang, PH;Carragher, NO;Munro, AF;Murphy, DJ;Veselkov, K;Seckl, MJ;Moffatt, MF;Cookson, WOC;Pardo, OE;
    Cell death & disease  2023-11-08
  7. Analysis of proteins released from osteoarthritic cartilage by compressive loading
    Authors: Tsuno, H;Tanaka, N;Naito, M;Ohashi, S;Iwasawa, M;Kadoguchi, T;Mitomi, H;Matsui, T;Furukawa, H;Fukui, N;
    Scientific reports  2023-10-25
  8. In need of age-appropriate cardiac models: Impact of cell age on extracellular matrix therapy outcomes
    Authors: Ozcebe, SG;Zorlutuna, P;
    Aging cell  2023-10-06
  9. Differential Effects of Pancreatic Cancer-Derived Extracellular Vesicles Driving a Suppressive Environment
    Authors: Purushothaman, A;Oliva-Ramírez, J;Treekitkarnmongkol, W;Sankaran, D;Hurd, MW;Putluri, N;Maitra, A;Haymaker, C;Sen, S;
    International journal of molecular sciences  2023-09-27
  10. C9orf72-ALS human iPSC microglia are pro-inflammatory and toxic to co-cultured motor neurons via MMP9
    Authors: Vahsen, BF;Nalluru, S;Morgan, GR;Farrimond, L;Carroll, E;Xu, Y;Cramb, KML;Amein, B;Scaber, J;Katsikoudi, A;Candalija, A;Carcolé, M;Dafinca, R;Isaacs, AM;Wade-Martins, R;Gray, E;Turner, MR;Cowley, SA;Talbot, K;
    Nature communications  2023-09-22
  11. Clonal MDS/AML cells with enhanced TWIST1 expression reprogram the differentiation of bone marrow MSCs
    Authors: Li, H;Wang, Y;Yang, F;Feng, S;Chang, K;Yu, X;Guan, F;Li, X;
    Redox biology  2023-09-21
  12. Age-related STAT3 signaling regulates severity of respiratory syncytial viral infection in human bronchial epithelial cells
    Authors: Zhao, C;Wang, W;Bai, Y;Amonkar, G;Mou, H;Olejnik, J;Hume, AJ;Mühlberger, E;Fang, Y;Que, J;Fearns, R;Ai, X;Lerou, PH;
    bioRxiv : the preprint server for biology  2023-09-21
  13. Telomere dysfunction promotes cholangiocyte senescence and biliary fibrosis in Primary Sclerosing Cholangitis
    Authors: Jalan-Sakrikar, N;Anwar, A;Yaqoob, U;Gan, C;Lagnado, AB;Wixom, AQ;Jurk, D;Huebert, RC;
    JCI insight  2023-09-14
  14. Free Bilirubin Induces Neuro-Inflammation in an Induced Pluripotent Stem Cell-Derived Cortical Organoid Model of Crigler-Najjar Syndrome
    Authors: Pranty, AI;Wruck, W;Adjaye, J;
    Cells  2023-09-14
  15. Human lncRNA SUGCT-AS1 Regulates the Proinflammatory Response of Macrophage
    Authors: Lim, YH;Yoon, G;Ryu, Y;Jeong, D;Song, J;Kim, YS;Ahn, Y;Kook, H;Kim, YK;
    International journal of molecular sciences  2023-08-28
  16. Effect of Inflammatory Microenvironment on the Regenerative Capacity of Adipose-Derived Mesenchymal Stem Cells
    Authors: Sz?cs, D;Miklós, V;Monostori, T;Guba, M;Kun-Varga, A;Póliska, S;Kis, E;Bende, B;Kemény, L;Veréb, Z;
    Cells  2023-07-29
  17. Herpes Simplex Virus Infection Alters the Immunological Properties of Adipose-Tissue-Derived Mesenchymal-Stem Cells
    Authors: Kun-Varga, A;Gubán, B;Miklós, V;Parvaneh, S;Guba, M;Sz?cs, D;Monostori, T;Varga, J;Varga, Á;Rázga, Z;Bata-Csörg?, Z;Kemény, L;Megyeri, K;Veréb, Z;
    International journal of molecular sciences  2023-07-26
  18. Atezolizumab Induces Necroptosis and Contributes to Hepatotoxicity of Human Hepatocytes
    Authors: Endo, Y;Winarski, KL;Sajib, MS;Ju, A;Wu, WJ;
    International journal of molecular sciences  2023-07-20
  19. IL1R1+ cancer-associated fibroblasts drive tumor development and immunosuppression in colorectal cancer
    Authors: Koncina, E;Nurmik, M;Pozdeev, VI;Gilson, C;Tsenkova, M;Begaj, R;Stang, S;Gaigneaux, A;Weindorfer, C;Rodriguez, F;Schmoetten, M;Klein, E;Karta, J;Atanasova, VS;Grzyb, K;Ullmann, P;Halder, R;Hengstschläger, M;Graas, J;Augendre, V;Karapetyan, YE;Kerger, L;Zuegel, N;Skupin, A;Haan, S;Meiser, J;Dolznig, H;Letellier, E;
    Nature communications  2023-07-17
  20. Endocrine therapy synergizes with SMAC mimetics to potentiate antigen presentation and tumor regression in hormone receptor-positive breast cancer
    Authors: Hermida-Prado, F;Xie, Y;Sherman, S;Nagy, Z;Russo, D;Akhshi, T;Chu, Z;Feit, A;Campisi, M;Chen, M;Nardone, A;Guarducci, C;Lim, K;Font-Tello, A;Lee, I;García-Pedrero, J;Canadas, I;Agudo, J;Huang, Y;Sella, T;Jin, Q;Tayob, N;Mittendorf, EA;Tolaney, SM;Qiu, X;Long, H;Symmans, WF;Lin, JR;Santagata, S;Bedrosian, I;Yardley, DA;Mayer, IA;Richardson, ET;Oliveira, G;Wu, CJ;Schuster, EF;Dowsett, M;Welm, AL;Barbie, D;Metzger, O;Jeselsohn, R;
    Cancer research  2023-07-14
  21. Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia
    Authors: Park, CS;Yoshihara, H;Gao, Q;Qu, C;Iacobucci, I;Ghate, PS;Connelly, JP;Pruett-Miller, SM;Wagner, B;Robinson, CG;Mishra, A;Peng, J;Yang, L;Rankovic, Z;Finkelstein, D;Luger, S;Litzow, M;Paietta, EM;Hebbar, N;Velasquez, MP;Mullighan, CG;
    Cell reports  2023-07-14
  22. HMGB1, a potential regulator of tumor microenvironment in KSHV-infected endothelial cells
    Authors: Lee, MJ;Park, J;Choi, S;Yoo, SM;Park, C;Kim, HS;Lee, MS;
    Frontiers in microbiology  2023-07-13
  23. Simultaneous isolation of hormone receptor positive breast cancer organoids and fibroblasts reveals stroma-mediated resistance mechanisms
    Authors: Hogstrom, JM;Cruz, KA;Selfors, LM;Ward, MN;Mehta, TS;Kanarek, N;Philips, J;Dialani, V;Wulf, G;Collins, LC;Patel, JM;Muranen, T;
    The Journal of biological chemistry  2023-07-07
  24. Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome
    Authors: Cozzolino, K;Sanford, L;Hunter, S;Molison, K;Erickson, B;Jones, T;Ajit, D;Galbraith, MD;Espinosa, JM;Bentley, DL;Allen, MA;Dowell, RD;Taatjes, DJ;
    bioRxiv : the preprint server for biology  2023-07-05
  25. Gene Expression Changes in Cytokine and Chemokine Receptors in Association with Melanoma Liver Metastasis
    Authors: Koroknai, V;Sz�sz, I;Bal�zs, M;
    International journal of molecular sciences  2023-05-17
  26. TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseases
    Authors: Kim, J;Lim, J;Yoo, ID;Park, S;Moon, JS;
    Redox biology  2023-05-06
  27. Self-assembled peptide-substance P hydrogels alleviate inflammation and ameliorate the cartilage regeneration in knee osteoarthritis
    Authors: Kim, SJ;Kim, JE;Choe, G;Song, DH;Kim, SJ;Kim, TH;Yoo, J;Kim, SH;Jung, Y;
    Biomaterials research  2023-05-04
  28. Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells
    Authors: AL Pourzia, ML Olson, SR Bailey, AC Boroughs, A Aryal, J Ryan, MV Maus, A Letai
    Cell Death & Disease, 2023-04-13;14(4):267.  2023-04-13
  29. miRNome and Proteome Profiling of Human Keratinocytes and Adipose Derived Stem Cells Proposed miRNA-Mediated Regulations of Epidermal Growth Factor and Interleukin 1-Alpha
    Authors: H Shahin, S Abdallah, J Das, W He, I El-Serafi, I Steinvall, F Sjöberg, M Elmasry, AT El-Serafi
    International Journal of Molecular Sciences, 2023-03-04;24(5):.  2023-03-04
  30. Extracellular Vesicles Derived from Three-Dimensional-Cultured Human Umbilical Cord Blood Mesenchymal Stem Cells Prevent Inflammation and Dedifferentiation in Pancreatic Islets
    Authors: E Lee, S Ha, G Kim, JH Kim, SM Jin
    Stem Cells International, 2023-02-20;2023(0):5475212.  2023-02-20
  31. gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells
    Authors: AJ Suh, DI Suzuki, SG Gychka, TI Brelidze, YJ Suzuki
    International Journal of Molecular Sciences, 2023-02-10;24(4):.  2023-02-10
  32. Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury
    Authors: ME Mohr, S Li, AM Trouten, RA Stairley, PL Roddy, C Liu, M Zhang, HM Sucov, G Tao
    bioRxiv : the preprint server for biology, 2023-02-08;0(0):.  2023-02-08
  33. Tumor-secreted exosomal miR-141 activates tumor-stroma interactions and controls premetastatic niche formation in ovarian cancer metastasis
    Authors: Y Mo, LL Leung, CSL Mak, X Wang, WS Chan, LMN Hui, HWM Tang, MKY Siu, R Sharma, D Xu, SKW Tsui, HYS Ngan, MMH Yung, KKL Chan, DW Chan
    Molecular Cancer, 2023-01-09;22(1):4.  2023-01-09
  34. gp120 envelope glycoproteins of HIV-1 Group M Subtype A and Subtype B differentially affect gene expression in human vascular endothelial cells
    Authors: AJ Suh, DI Suzuki, SG Gychka, TI Brelidze, YJ Suzuki
    bioRxiv : the preprint server for biology, 2023-01-03;0(0):.  2023-01-03
  35. Pericytes take up and degrade alpha-synuclein but succumb to apoptosis under cellular stress
    Authors: TJ Stevenson, RH Johnson, J Savistchen, J Rustenhove, Z Woolf, LCD Smyth, HC Murray, RLM Faull, J Correia, P Schweder, P Heppner, C Turner, R Melki, BV Dieriks, MA Curtis, M Dragunow
    Scientific Reports, 2022-10-15;12(1):17314.  2022-10-15
  36. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
    Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
    Nature Communications, 2022-10-14;13(1):6078.  2022-10-14
  37. The Differential Paracrine Role of the Endothelium in Prostate Cancer Cells
    Authors: V Torres-Est, M Mastri, S Rosario, P Fuenzalida, CE Echeverría, E Flores, A Watts, J Cerda-Infa, VP Montecinos, PC Sotomayor, J Amigo, C Escudero, F Nualart, JML Ebos, DJ Smiraglia, AS Godoy
    Cancers, 2022-09-29;14(19):.  2022-09-29
  38. The Nicotinic Receptor Polymorphism rs16969968 Is Associated with Airway Remodeling and Inflammatory Dysregulation in COPD Patients
    Authors: L Saber Cher, Z Diabasana, JM Perotin, J Ancel, LMG Petit, MA Devilliers, A Bonnomet, N Lalun, G Delepine, U Maskos, P Gosset, M Polette, A Muggeo, T Guillard, G Deslée, V Dormoy
    Cells, 2022-09-20;11(19):.  2022-09-20
  39. ADSCs stimulated by resistin promote breast cancer cell malignancy via CXCL5 in a breast cancer coculture model
    Authors: YY Wang, AC Hung, YC Wu, S Lo, HD Chen, YK Chen, YC Hsieh, SC Hu, MF Hou, SF Yuan
    Scientific Reports, 2022-09-14;12(1):15437.  2022-09-14
  40. Melanoma Mediated Disruption of Brain Endothelial Barrier Integrity Is Not Prevented by the Inhibition of Matrix Metalloproteinases and Proteases
    Authors: A Anchan, G Finlay, CE Angel, JJW Hucklesby, SE Graham
    Biosensors, 2022-08-19;12(8):.  2022-08-19
  41. During early stages of cancer, neutrophils initiate anti-tumor immune responses in tumor-draining lymph nodes
    Authors: E Pylaeva, G Korschunow, I Spyra, S Bordbari, E Siakaeva, I Ozel, M Domnich, A Squire, A Hasenberg, K Thangavelu, T Hussain, M Goetz, KS Lang, M Gunzer, W Hansen, J Buer, A Bankfalvi, S Lang, J Jablonska
    Cell Reports, 2022-08-16;40(7):111171.  2022-08-16
  42. Cell-Type Dependent Regulation of the Electrogenic Na+/HCO3- Cotransporter 1 (NBCe1) by Hypoxia and Acidosis in Glioblastoma
    Authors: M Giannaki, DE Ruf, E Pfeifer, K Everaerts, DH Heiland, O Schnell, CR Rose, E Roussa
    International Journal of Molecular Sciences, 2022-08-11;23(16):.  2022-08-11
  43. Plasmid-mediated gene transfer of Cas9 induces vector-related but not SpCas9-related immune responses in human retinal pigment epithelial cells
    Authors: JK Pfromm, M Bonillo, D Dauletbeko, K Bucher, MD Fischer
    Scientific Reports, 2022-08-01;12(1):13202.  2022-08-01
  44. Human iPSC co-culture model to investigate the interaction between microglia and motor neurons
    Authors: BF Vahsen, E Gray, A Candalija, KML Cramb, J Scaber, R Dafinca, A Katsikoudi, Y Xu, L Farrimond, R Wade-Marti, WS James, MR Turner, SA Cowley, K Talbot
    Scientific Reports, 2022-07-23;12(1):12606.  2022-07-23
  45. Targeting HIC1/TGF-beta axis-shaped prostate cancer microenvironment restrains its progression
    Authors: T Wu, W Wang, G Shi, M Hao, Y Wang, M Yao, Y Huang, L Du, X Zhang, D Ye, X Bian, J Wang
    Cell Death & Disease, 2022-07-19;13(7):624.  2022-07-19
  46. Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistance
    Authors: R Islam, J Mishra, NS Polavaram, S Bhattachar, Z Hong, S Bodas, S Sharma, A Bouska, T Gilbreath, AM Said, LM Smith, BA Teply, MH Muders, SK Batra, K Datta, S Dutta
    Oncogene, 2022-07-19;40(3):111097.  2022-07-19
  47. Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells
    Authors: N Igarashi, K Miyata, TM Loo, M Chiba, A Hanyu, M Nishio, H Kawasaki, H Zheng, S Toyokuni, S Kon, K Moriyama, Y Fujita, A Takahashi
    Oncogene, 2022-07-18;13(1):4157.  2022-07-18
  48. Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
    Authors: H Stölting, L Baillon, R Frise, K Bonner, RJ Hewitt, PL Molyneaux, ML Gore, Breathing, WS Barclay, S Saglani, CM Lloyd
    Mucosal Immunology, 2022-07-15;0(0):.  2022-07-15
  49. The human amniotic epithelium confers a bias to differentiate toward the neuroectoderm lineage in human embryonic stem cells
    Authors: D Ávila-Gonz, W Portillo, CP Barragán-Á, G Hernandez-, E Flores-Gar, A Molina-Her, NE Díaz-Martí, NF Díaz
    Elife, 2022-07-11;11(0):.  2022-07-11
  50. Interleukin-8 produced from cancer-associated fibroblasts suppresses proliferation of the OCUCh-LM1 cancer cell line
    Authors: R Tanaka, K Kimura, S Eguchi, G Ohira, S Tanaka, R Amano, H Tanaka, M Yashiro, M Ohira, S Kubo
    BMC Cancer, 2022-07-08;22(1):748.  2022-07-08
  51. The kinase activity of integrin-linked kinase regulates cellular senescence in gastric cancer
    Authors: C Ji, M Zhang, J Hu, C Cao, Q Gu, Y Liu, X Li, D Xu, L Ying, Y Yang, H Gao, J Li, L Yu
    Cell Death & Disease, 2022-07-01;13(7):577.  2022-07-01
  52. Two New Potential Therapeutic Approaches in Radiation Cystitis Derived from Mesenchymal Stem Cells: Extracellular Vesicles and Conditioned Medium
    Authors: C Helissey, N Guitard, H Théry, S Goulinet, P Mauduit, M Girleanu, AL Favier, M Drouet, C Parnot, C Chargari, S Cavallero, S François
    Biology, 2022-06-28;11(7):.  2022-06-28
  53. Serum biomarkers of neuroinflammation and blood-brain barrier leakage in amyotrophic lateral sclerosis
    Authors: MC Cao, EE Cawston, G Chen, C Brooks, J Douwes, D McLean, ES Graham, M Dragunow, EL Scotter
    BMC neurology, 2022-06-11;22(1):216.  2022-06-11
  54. Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics
    Authors: C Giannasi, L Mangiavini, S Niada, A Colombo, E Della Mort, V Vismara, A Ambrosanio, P Savadori, S Casati, GM Peretti, AT Brini
    Pharmaceutics, 2022-06-10;14(6):.  2022-06-10
  55. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions
    Authors: L Brandolini, M d'Angelo, R Novelli, V Castelli, C Giorgio, A Sirico, P Cocchiaro, F D'Egidio, E Benedetti, C Cristiano, A Bugatti, A Ruocco, PG Amendola, C Talarico, C Manelfi, D Iaconis, A Beccari, AU Quadros, TM Cunha, A Caruso, R Russo, A Cimini, A Aramini, M Allegretti
    Cell Death & Disease, 2022-05-25;13(5):500.  2022-05-25
  56. LCN2 is a new diagnostic biomarker and potential therapeutic target in idiopathic short stature
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    Journal of Cellular and Molecular Medicine, 2022-05-24;0(0):.  2022-05-24
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  137. Tumor-associated reactive astrocytes aid the evolution of immunosuppressive environment in glioblastoma
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  138. Paradoxical effects of JZL184, an inhibitor of monoacylglycerol lipase, on bone remodelling in healthy and cancer-bearing mice
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  144. Ectoine-Containing Inhalation Solution versus Saline Inhalation Solution in the Treatment of Acute Bronchitis and Acute Respiratory Infections: A Prospective, Controlled, Observational Study
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  148. USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and ?-TrCP and promotes cancer malignancy
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    Nat Commun, 2018-09-28;9(1):3996.  2018-09-28
  149. Ca2+ Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
    Authors: K Eubler, C Herrmann, A Tiefenbach, FM Köhn, JU Schwarzer, L Kunz, A Mayerhofer
    Int J Mol Sci, 2018-09-19;19(9):.  2018-09-19
  150. Gle1 mediates stress granule-dependent survival during chemotoxic stress
    Authors: L Glass, SR Wente
    Adv Biol Regul, 2018-09-18;0(0):.  2018-09-18
  151. Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines
    Authors: M Kusunoki, E Sata, K Nishio, T Tanaka, T Nishida, N Sugano, S Sato, M Asano
    Int J Med Sci, 2018-09-07;15(12):1365-1372.  2018-09-07
  152. Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
    Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
    Cell Rep, 2018-08-28;24(9):2300-2311.  2018-08-28
  153. A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
    Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
    J Oral Sci, 2018-08-25;0(0):.  2018-08-25
  154. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018-08-15;9(1):3267.  2018-08-15
  155. Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
    Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
    Cancer Med, 2018-08-09;0(0):.  2018-08-09
  156. Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
    Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
    Front Physiol, 2018-07-31;9(0):1025.  2018-07-31
  157. Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms
    Authors: C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu
    J. Cell. Mol. Med., 2018-07-05;0(0):.  2018-07-05
  158. IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
    Authors: A Calcinotto, C Spataro, E Zagato, D Di Mitri, V Gil, M Crespo, G De Bernard, M Losa, M Mirenda, E Pasquini, A Rinaldi, S Sumanasuri, MB Lambros, A Neeb, R Lucianò, CA Bravi, D Nava-Rodri, D Dolling, T Prayer-Gal, A Ferreira, A Briganti, A Esposito, S Barry, W Yuan, A Sharp, J de Bono, A Alimonti
    Nature, 2018-06-27;0(0):.  2018-06-27
  159. Unique and shared inflammatory profiles of human brain endothelia and pericytes
    Authors: LCD Smyth, J Rustenhove, TI Park, P Schweder, D Jansson, PA Heppner, SJ O'Carroll, EW Mee, RLM Faull, M Curtis, M Dragunow
    J Neuroinflammation, 2018-05-11;15(1):138.  2018-05-11
  160. Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
    Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
    Cell Death Dis, 2018-05-01;9(5):506.  2018-05-01
  161. CST3 and GDF15 ameliorate renal fibrosis by inhibiting fibroblast growth and activation
    Authors: YI Kim, HW Shin, YS Chun, JW Park
    Biochem. Biophys. Res. Commun., 2018-04-13;0(0):.  2018-04-13
  162. Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro
    Authors: MD Wood, J Mukherjee, RO Pieper
    Sci Rep, 2018-04-11;8(1):5805.  2018-04-11
  163. Identification and Complete Stereochemical Assignments of the New Resolvin Conjugates in Tissue Regeneration in Human Tissues that Stimulate Proresolving Phagocyte Functions and Tissue Regeneration
    Authors: X de la Rosa, PC Norris, N Chiang, AR Rodriguez, BW Spur, CN Serhan
    Am. J. Pathol., 2018-04-01;188(4):950-966.  2018-04-01
  164. Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
    Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
    Sci Adv, 2018-02-21;4(2):e1701729.  2018-02-21
  165. Withaferin A Associated Differential Regulation of Inflammatory Cytokines
    Authors: S Dubey, H Yoon, MS Cohen, P Nagarkatti, M Nagarkatti, D Karan
    Front Immunol, 2018-02-09;9(0):195.  2018-02-09
  166. Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine
    Authors: P Chang, B Zhang, L Shao, W Song, W Shi, L Wang, T Xu, D Li, X Gao, Y Qu, L Dong, J Wang
    Cell Death Dis, 2018-02-05;9(2):154.  2018-02-05
  167. ATP-mediated Events in Peritubular Cells Contribute to Sterile Testicular Inflammation
    Authors: L Walenta, D Fleck, T Fröhlich, H von Eysmon, GJ Arnold, J Spehr, JU Schwarzer, FM Köhn, M Spehr, A Mayerhofer
    Sci Rep, 2018-01-23;8(1):1431.  2018-01-23
  168. Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells
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    Sci Rep, 2018-01-19;8(1):1171.  2018-01-19
  169. Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts
    Authors: T Kifune, H Ito, M Ishiyama, S Iwasa, H Takei, T Hasegawa, M Asano, T Shirakawa
    J Oral Sci, 2018-01-01;60(4):519-525.  2018-01-01
  170. Obesity Enhances the Conversion of Adipose-Derived Stromal/Stem Cells into Carcinoma-Associated Fibroblast Leading to Cancer Cell Proliferation and Progression to an Invasive Phenotype
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  171. Lack of Evidence for Vasoactive and Inflammatory Mediators in the Promotion of Macular Edema Associated with Epiretinal Membranes
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  172. Histone Deacetylase 1 Depletion Activates Human Cardiac Mesenchymal Stromal Cell Proangiogenic Paracrine Signaling Through a Mechanism Requiring Enhanced Basic Fibroblast Growth Factor Synthesis and Secretion
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  2. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

View all Proteome Profiler Antibody Array FAQs

Reviews for Proteome Profiler Human XL Cytokine Array Kit

Average Rating: 4.7 (Based on 20 Reviews)

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Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 12/11/2023

LI-COR

Proteome Profiler Human XL Cytokine Array Kit
By Dong Hu on 07/11/2022
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 05/13/2022

Product was easy to use and quick. In house analysis software would improve the process. Array has not been tested if the specific antibodies differentiate between precursor and mature proteins or bound vs unbound proteins. Testing this would increase applicability. Image provided is an example of 100 ul of human serum.

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 12/02/2021
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 10/21/2021
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 09/07/2021
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 04/29/2021

Used 1 ml of pooled basal media from air-liquid interface cultures of primary epithelial cells

Protocol very easy to follow, but data analysis can be tricky. Would benefit massively from coming with its own analysis tool / ImageJ plugin.

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 10/20/2020
Proteome Profiler Human XL Cytokine Array Kit
By Dhanush Haspula on 05/22/2020

220 uL of rat plasma for overnight incubation with arrays at 4c.

Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Louise Reynolds on 02/18/2020

Human endothelial cells treated +/- FAK kinase inhibitors +/- doxorubicin

Proteome Profiler Human XL Cytokine Array Kit
By Lucia Lisi on 01/14/2020

Chiavari M, Ciotti GMP, Navarra P, Lisi L. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line. Brain Sci. 2019;9(5):111. Published 2019 May 15. doi:10.3390/brainsci9050111

Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Fadi Saadeh on 07/01/2019
Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 06/07/2019
Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 06/06/2019
Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 02/07/2019
Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Ivan Luzardo on 01/03/2019
Proteome Profiler Human XL Cytokine Array Kit
By Samantha Scarneo on 12/26/2018
Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Scott Scarneo on 12/12/2018

THP-1 Cells stimulated with LPS+IFN gamma for 24 hours.

Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 09/14/2018
Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Leslie Priddy on 04/01/2018