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Rat C-Reactive Protein/CRP DuoSet ELISA

Catalog #: DY1744
11 Citations Datasheet / COA / SDS

Discontinued Product

DY1744 has been discontinued.
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Rat C-Reactive Protein / CRP ELISA Standard Curve
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Citations (11)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Rat C-Reactive Protein/CRP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
156.0 - 10,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat C-Reactive Protein/CRP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Rat C-Reactive Protein / CRP ELISA Standard Curve View Larger

Rat C-Reactive Protein / CRP ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Entrez Gene IDs:
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names:
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Rat C-Reactive Protein/CRP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Miconazole Mitigates Acetic Acid-Induced Experimental Colitis in Rats: Insight into Inflammation, Oxidative Stress and Keap1/Nrf-2 Signaling Crosstalk
    Authors: IA Alsharif, HM Fayed, RF Abdel-Rahm, RM Abd-Elsala, HA Ogaly
    Biology, 2022-02-13;11(2):.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations
    Authors: L Zizmare, CN Boyle, S Buss, S Louis, L Kuebler, K Mulay, R Krüger, L Steinhauer, I Mack, M Rodriguez, K Herfert, Y Ritze, C Trautwein
    International Journal of Molecular Sciences, 2022-01-20;23(3):.
    Species: Rat
    Sample Types: Feces
  3. Renoprotection Induced by Aerobic Training Is Dependent on Nitric Oxide Bioavailability in Obese Zucker Rats
    Authors: RVP Neves, HL Corrêa, IV de Sousa N, MK Souza, F Costa, AS Haro, LA Deus, AL Reis, HG Simões, RV Andrade, CO Assumpção, W Stone, J Prestes, EC Vieira, R de Cássia, V Cruzat, TS Rosa
    Oxidative Medicine and Cellular Longevity, 2021-09-28;2021(0):3683796.
    Species: Rat
    Sample Types: Tissue Homogenates
  4. Formononetin ameliorates muscle atrophy by regulating myostatin-mediated PI3K/Akt/FoxO3a pathway and satellite cell function in chronic kidney disease
    Authors: L Liu, R Hu, H You, J Li, Y Liu, Q Li, X Wu, J Huang, X Cai, M Wang, L Wei
    Journal of Cellular and Molecular Medicine, 2021-01-06;0(0):.
    Species: Rat
    Sample Types: Serum
  5. Exercise-Induced Circulating Irisin Level Is Correlated with Improved Cardiac Function in Rats
    Authors: DY Seo, JH Bae, TN Kim, HB Kwak, PT Kha, J Han
    Int J Environ Res Public Health, 2020-05-29;17(11):.
    Species: Rat
    Sample Types: Plasma
  6. Effect of ulinastatin on myocardial ischemia-reperfusion injury through JNK and P38 MAPK signaling pathways
    Authors: ZH Yang, YJ Lu, KP Gu, ZY Xiang, HM Huang
    Eur Rev Med Pharmacol Sci, 2019-10-01;23(19):8658-8664.
    Species: Rat
    Sample Types: Serum
  7. Atractylenolide III Attenuates Muscle Wasting in Chronic Kidney Disease via the Oxidative Stress-Mediated PI3K/AKT/mTOR Pathway
    Authors: M Wang, R Hu, Y Wang, L Liu, H You, J Zhang, X Wu, T Pei, F Wang, L Lu, W Xiao, L Wei
    Oxid Med Cell Longev, 2019-04-18;2019(0):1875471.
    Species: Rat
    Sample Types: Serum
  8. Role of methotrexate chronotherapy in collagen-induced rheumatoid arthritis in�rats
    Authors: X Wang
    Z Rheumatol, 2018-04-01;0(0):.
    Species: Rat
    Sample Types: Serum
  9. Xiexin Tang improves the symptom of type 2 diabetic rats by modulation of the gut microbiota
    Authors: X Wei, J Tao, S Xiao, S Jiang, E Shang, Z Zhu, D Qian, J Duan
    Sci Rep, 2018-02-27;8(1):3685.
    Species: Rat
    Sample Types: Serum
  10. Periodontitis-activated monocytes/macrophages cause aortic inflammation.
    Authors: Miyajima S, Naruse K, Kobayashi Y, Nakamura N, Nishikawa T, Adachi K, Suzuki Y, Kikuchi T, Mitani A, Mizutani M, Ohno N, Noguchi T, Matsubara T
    Sci Rep, 2014-06-04;4(0):5171.
    Species: Rat
    Sample Types: Serum
  11. Hyperglycemia is associated with enhanced gluconeogenesis in a rat model of permanent cerebral ischemia.
    Authors: Wang Y, Chen C, Lin S, Chuang Y, Sheu W, Tung K
    Mol Cell Endocrinol, 2012-12-29;367(1):50-6.
    Species: Rat
    Sample Types: Plasma

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