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Rat MAG/Siglec-4a Antibody

Catalog #: AF538
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF538
AF538-SP
MAG/Siglec‑4a in Rat Brain.
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Citations (6)
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Rat MAG/Siglec-4a Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat MAG/Siglec-4a in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MAG/Siglec-4a
Gly20-Pro516
Accession # P07722
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat MAG/Siglec‑4a Fc Chimera (Catalog # 538-MG)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry MAG/Siglec‑4a antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

MAG/Siglec‑4a in Rat Brain. MAG/Siglec‑4a was detected in perfusion fixed frozen sections of rat brain (cerebellum) using 25 µg/mL Goat Anti-Rat MAG/Siglec‑4a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF538) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to processes of oligodendrocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAG/Siglec-4a

MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell-surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.

References
  1. Kelm, S. et al. (1994) Current Biology 4:965.
  2. McKerracher, L. et al. (1994) Neuron 13:805.
  3. Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
  4. Cai, D. et al. (1999) Neuron 22:89.
Long Name
Myelin-associated Glycoprotein
Entrez Gene IDs
4099 (Human); 17136 (Mouse); 29409 (Rat)
Alternate Names
GMAsialic acid binding Ig-like lectin 4A; MAG; myelin associated glycoprotein; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A; Siglec4a; Siglec-4a; S-MAG

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Citations for Rat MAG/Siglec-4a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization
    Authors: VK Kuna, A Lundgren, LO Anerillas, P Kelk, M Brohlin, M Wiberg, PJ Kingham, LN Novikova, G Andersson, LN Novikov
    International Journal of Molecular Sciences, 2022-08-06;23(15):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Nonneuronal control of the differential distribution of myelin along retinal ganglion cell axons in the mouse.
    Authors: Yang, Xiaoli, Zou, Haidong, Jung, Gila, Richard, Gisbert, Linke, Stephan, Ader, Marius, Bartsch, Udo
    Invest Ophthalmol Vis Sci, 2013-12-02;54(13):7819-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. In vitro expanded stem cells from the developing retina fail to generate photoreceptors but differentiate into myelinating oligodendrocytes.
    Authors: Czekaj M, Haas J, Gebhardt M, Muller-Reichert T, Humphries P, Farrar J, Bartsch U, Ader M
    PLoS ONE, 2012-07-25;7(7):e41798.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Netrin-1 is a novel myelin-associated inhibitor to axon growth.
    Authors: Löw K, Culbertson M, Bradke F, Tessier-Lavigne M, Tuszynski MH
    J. Neurosci., 2008-01-30;28(5):1099-108.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Cleavage of myelin associated glycoprotein by matrix metalloproteinases.
    Authors: Milward E, Kim KJ, Szklarczyk A, Nguyen T, Melli G, Nayak M, Deshpande D, Fitzsimmons C, Hoke A, Kerr D, Griffin JW, Calabresi PA, Conant K
    J. Neuroimmunol., 2007-12-11;193(1):140-8.
    Species: Rat
    Sample Types: Recombinant Protein
    Applications: Western Blot
  6. Neuregulin 1-erbB signaling is necessary for normal myelination and sensory function.
    Authors: Chen S, Velardez MO, Warot X, Yu ZX, Miller SJ, Cros D, Corfas G
    J. Neurosci., 2006-03-22;26(12):3079-86.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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