Rat Nestin Antibody

Catalog #: AF2736
10 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2736
AF2736-SP

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All Nestin Antibodies

Product Details

Citations (10)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Nestin Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat Nestin

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of rat embyo (E15)

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. beta -III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta -III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

View Larger

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nestin

Nestin is a 240 kDa class VI intermediate filament protein that was first identified in neuroepithelial stem cells (1, 2). It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% amino acid sequence homology with the mouse protein.

References

Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
Lendahl, U. et al. (1990) Cell 60:585.

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Alternate Names

FLJ21841; NES; Nestin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Rat Nestin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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The Rb/E2F axis is a key regulator of the molecular signatures instructing the quiescent and activated adult neural stem cell state
Authors: BC Fong, I Chakroun, MA Iqbal, S Paul, J Bastasic, D O'Neil, E Yakubovich, AT Bejjani, N Ahmadi, A Carter, A Clark, G Leone, DS Park, N Ghanem, R Vandenbosc, RS Slack
Cell Reports, 2022-11-01;41(5):111578.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells
Authors: L Chen, M Zhang, L Fang, X Yang, N Cao, L Xu, L Shi, Y Cao
The Journal of Biological Chemistry, 2021-10-04;297(5):101275.
Species: Mouse
Sample Types: Whole Cells
Applications: IF
Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recovery
Authors: K Xi, Y Gu, J Tang, H Chen, Y Xu, L Wu, F Cai, L Deng, H Yang, Q Shi, W Cui, L Chen
Nat Commun, 2020-09-09;11(1):4504.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Conditional inactivation of Pen-2 in the developing neocortex leads to rapid switch of apical progenitors to basal progenitors
Authors: S Cheng, T Liu, Y Hu, Y Xia, J Hou, C Huang, X Zou, J Liang, Y Stone Shi, Y Zheng, J Lu, G Chen
J. Neurosci., 2019-01-28;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex
Authors: Y Takamori, Y Hirahara, T Wakabayash, T Mori, T Koike, Y Kataoka, Y Tamura, S Kurebayash, K Kurokawa, H Yamada
IBRO Rep, 2018-11-05;5(0):99-109.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr
Nestin-positive microglia in adult rat cerebral cortex.
Authors: Takamori Y, Mori T, Wakabayashi T, Nagasaka Y, Matsuzaki T, Yamada H
Brain Res., 2009-03-21;1270(0):10-8.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr
Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis.
Authors: Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M, Garcia AD, Sofroniew MV, Carmichael ST, Kornblum HI, Liu X, Wu H
J. Neurosci., 2009-02-11;29(6):1874-86.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Mechanisms of murine lacrimal gland repair after experimentally induced inflammation.
Authors: Zoukhri D, Fix A, Alroy J, Kublin CL
Invest. Ophthalmol. Vis. Sci., 2008-06-27;49(10):4399-406.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
Biotechnol. Bioeng., 2007-08-15;97(6):1617-25.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary.
Authors: Subburaju S, Aguilera G
Endocrinology, 2007-04-05;148(7):3102-10.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr