Recombinant Human Neurofascin Protein, CF Summary
Product Specifications
Ile25-Trp1039, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8208-NF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Neurofascin
Neurofascin, a type I transmembrane glycoprotein, is a member of the L1 family of cell adhesion molecules (CAMs) (1-4). L1CAM family members are composed of an extracellular domain (ECD) that contains six immunoglobulin (Ig)-like domains and multiple fibronectin type III repeats, followed by transmembrane and cytoplasmic domains (2, 4, 5). Multiple isoforms of Neurofascin, including NF155, NF166, NF180, and NF186, can be generated by alternative splicing with a predicted range of approximately 70 kDa to 150 kDa (4). These isoforms differ in the combination of fibronectin type III repeats, as well as in the presence of a proline-, alanine-, and threonine-rich segment (PAT domain) located just after the fourth fibronectin type III repeat (4). This recombinant human Neurofascin protein is most close to NF155 and shares 96% amino acid sequence identity with comparable regions of rat and mouse homologs. In rats, NF155 is transiently expressed by oligodendrocytes and Schwann cells during axon myelination (6, 7). NF155 clusters in paranodal regions of oligodendroglia and binds to the Caspr-Contactin complex located on the adjacent axon to form and stabilize paranodal axoglial junctions (8-11). It has been suggested that the ECD of NF155 must be cleaved from oligodendroglia membranes to form and/or stabilize the paranodal structure (12). NF155 has also been shown to promote neuronal adhesion and neurite outgrowth in rats and chickens (12-14). Alterations in NF155 expression have been associated with multiple sclerosis (15).
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- Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
- Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
- Kriebel, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:694.
- Liu, H. et al. (2011) J. Biol. Chem. 286:797.
- Collinson, J.M. et al. (1998) Glia 23:11.
- Basak, S. et al. (2007) Dev. Biol. 311:408.
- Charles, P. et al. (2002) Curr. Biol. 12:217.
- Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
- Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
- Sherman, D.L. et al. (2005) Neuron 48:737.
- Maier, O. et al. (2006) Exp. Cell Res. 312:500.
- Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
- Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
- Howell, O.W. et al. (2006) Brain 129:3173.
Citations for Recombinant Human Neurofascin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Immunoadsorption and Plasma Exchange in Seropositive and Seronegative Immune-Mediated Neuropathies
Authors: AJ Davies, J Fehmi, M Senel, H Tumani, J Dorst, S Rinaldi
J Clin Med, 2020-06-27;9(7):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA(Detection) -
IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay
Authors: Katsunori Ikewaki
J. Neuroimmunol., 2016-11-09;0(0):.
Species: Human
Sample Types: Serum
Applications: Bioassay
FAQs
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Which isoform of Neurofascin is 8208-NF?
8208-NF corresponds to Accession number O94856-3. Accession number O94856-3 is highly similar to O94856-8, which is also known as NF155. Isoform NF140 contains FNIII domains 1, 2, and 4, and no mucin domain, but it lacks FNIII domain 3. Because 8208-NF contains FNIII domain 3, 8208-NF is not isoform NF140. 8208-NF is actually closest to NF155
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