Recombinant Mouse/Rat Noggin, Animal-Free Protein Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Qk033
| Stability & Storage: | Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles. |
Scientific Data
View Larger
Noggin is a BMP inhibitor and its activity is determined by inhibition of BMP2 (Qk007) activity in a BMP-2 responsive firefly luciferase reporter assay.HEK293T cells are treated (in triplicate) with a serial dilution of Noggin and standard concentration of BMP2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 62.7 ng/ml (1.4 nM).
View Larger
Noggin protein (Qk033) has an unusual migration in non-reduced (NR) SDS-PAGE due to the non-covalent dimer which is the active protein. Similar migration in SDS-PAGE is seen for Gremlin-1, a related BMP antagonist. The identity of the purified dimeric protein was confirmed using mass spectrometry. Upon reduction, the protein monomer migrates at 23 kDa.Purified recombinant mouse Noggin protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta -mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.
Reconstitution Calculator
Background: Noggin
Noggin is a secreted homodimeric antagonist of bone morphogenetic proteins (BMPs). It binds BMP-4 with high affinity and BMP-7 with lower affinity. Noggin regulates BMP activity in neural tube formation, somite patterning, cardiomyocyte development, and skeletal joint formation. Mutations of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. Noggin may also regulate the differentiation of embryonic stem cells into dopaminergic neurons.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse/Rat Noggin, Animal-Free Protein
There are currently no reviews for this product. Be the first to review Recombinant Mouse/Rat Noggin, Animal-Free Protein and earn rewards!
Have you used Recombinant Mouse/Rat Noggin, Animal-Free Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
