Recombinant Mouse TROY/TNFRSF19 Fc Chimera Protein, CF

Catalog #: 723-TR
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
723-TR-100

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TROY/TNFRSF19 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TROY/TNFRSF19 protein

Mouse TROY (Glu30-Leu170) Accession # Q9JLL3	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9JLL3

N-terminal Sequence
Analysis

Glu30

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

42.3 kDa (monomer)

SDS-PAGE

45-50 kDa, reducing conditions

Product Datasheets

723-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

723-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TROY/TNFRSF19

TROY (also known as TAJ and TNFRSF19) is a 45‑55 kDa member of the TNF receptor superfamily of molecules (1‑4). It is widely expressed in the embryo, but shows limited expression in adult. In particular, embryonic TROY is found in embryonic epithelium, neural tube, and mesenchyme, and developing neurons of the hippocampus and thalamus (5, 6). In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles where it is expressed by dermal papilla matrix cells (4, 7, 8, 9). In human, TROY has also been found on melanoma cells, not normal melanocytes (10). TROY is reportedly a component of the Nogo-A receptor, taking the place of p75 in a membrane-bound LINGO-1:NgR1:TROY complex (6, 8, 9). This complex purportedly mediates axon repulsion initiated by one of five myelin-associated inhibitory factors (OMpg; CD100; Ephrin B3; Nogo-A; MAG) (6, 11). In the hair follicle, TROY binds LTa and acts with Eda to drive secondary hair follicle development (4, 12). Mature human TROY is a 394 amino acid (aa) type I transmembrane protein (1-3). It possesses a 141 aa extracellular region (aa 30-170) that contains three 40 aa TNFR cysteine motifs, and an extended 232 aa cytoplasmic domain (aa 192-423). There is one potential isoform that shows a two aa substitution for aa 416-423. The extracellular domains of human and mouse TROY share 90% aa identity.

References

Hu, S. et al. (1999) Genomics 62:103.
Eby, M.T. et al. (2000) J. Biol. Chem. 275:15336.
Kojima, T. et al. (2000) J. Biol. Chem. 275:20742.
Hashimoto, T. et al. (2008) Cell Cycle 7:106.
Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
Mi, S. (2008) Cytokine Growth Factor Rev. 19:245.
Satoh, J. et al. (2007) Neuropathol. Appl. Neurobiol. 33:99.
Shao, Z. et al. (2005) Neuron 45:353.
Park, J.B. et al. (2000) Neuron 45:345.
Spanjaard, R.A. et al. (2007) Int. J. Cancer 120:1304.
Venkatesh, K. et al. (2007) J. Cell Biol. 177:393.
Pispa, J. et al. (2008) Hum. Mol. Genet. 17:3380.

Long Name

Tumor Necrosis Factor Receptor Superfamily, Member 19

Entrez Gene IDs

55504 (Human); 29820 (Mouse)

Alternate Names

TAJ; TAJ-alpha; TAJToxicity and JNK inducer; TNFRSF19; TRADE; TROY; TROYtumor necrosis factor receptor superfamily member 19; tumor necrosis factor receptor superfamily, member 19

Related Research Areas

Citation for Recombinant Mouse TROY/TNFRSF19 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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