Stop Solution 2N Sulfuric Acid
Stop Solution 2N Sulfuric Acid Summary
INTENDED USE & DESCRIPTION
Stop Solution (Part # 895032) contains 15 vials (6 mL/vial) of 2N sulfuric acid for the stopping the action of horseradish peroxidase on the tetramethylbenzidine substrate in an ELISA.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.
PROCEDURE
Add 50-100 µL/well to stop the action of horseradish peroxidase on the substrate, tetramethylbenzidine. If the color in the wells is green or the color change does not appear to be uniform, gently tap the plate to ensure thorough mixing.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
- Caution: Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.
Specifications
Product Datasheets
Citations for Stop Solution 2N Sulfuric Acid
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Impact of Immunopathy and Coagulopathy on Multi-Organ Failure and Mortality in a Lethal Porcine Model of Controlled and Uncontrolled Hemorrhage
Authors: Simovic, MO;Bynum, J;Liu, B;Dalle Lucca, JJ;Li, Y;
International journal of molecular sciences 2024-02-21
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Lipidation and PEGylation Strategies to Prolong the in Vivo Half-Life of a Nanomolar EphA4 Receptor Antagonist
Authors: Gomez-Soler, M;Olson, EJ;de la Torre, ER;Zhao, C;Lamberto, I;Flood, DT;Danho, W;Lechtenberg, BC;Riedl, SJ;Dawson, PE;Pasquale, EB;
European journal of medicinal chemistry 2023-12-15
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Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors: S Choi, Z Yang, Q Wang, Z Qiao, M Sun, J Wiggins, SH Xiang, Q Lu
Science Advances, 2023-01-27;9(4):eade2708. 2023-01-27
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SARS-CoV-2 vaccination-infection pattern imprints and diversifies T cell differentiation and neutralizing response against Omicron subvariants
Authors: J Wang, K Li, X Mei, J Cao, J Zhong, P Huang, Q Luo, G Li, R Wei, N Zhong, Z Zhao, Z Wang
Cell Discovery, 2022-12-21;8(1):136. 2022-12-21
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Active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model
Authors: DV Oliveira, KG Coupland, W Shao, S Jin, F Del Gaudio, S Wang, R Fox, JW Rutten, J Sandin, H Zetterberg, J Lundkvist, SA Lesnik Obe, U Lendahl, H Karlström
Embo Molecular Medicine, 2022-12-16;0(0):e16556. 2022-12-16
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The Soluble Fms-like Tyrosine Kinase-1 Contributes to Structural and Functional Changes in Endothelial Cells in Chronic Kidney Disease
Authors: A Schulz, CC Drost, B Hesse, K Beul, M Brand, GS Di Marco
International Journal of Molecular Sciences, 2022-12-16;23(24):. 2022-12-16
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Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
Authors: H Kim, B Jang, YJ Kim, JC Choi
International Journal of Molecular Sciences, 2022-09-11;23(18):. 2022-09-11
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Superimmunity by pan-sarbecovirus nanobodies
Authors: Y Xiang, W Huang, H Liu, Z Sang, S Nambulli, J Tubiana, KL Williams, WP Duprex, D Schneidman, IA Wilson, DJ Taylor, Y Shi
Cell Reports, 2022-06-08;39(13):111004. 2022-06-08
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Novel efficacious microRNA-30c analogs reduce apolipoprotein B secretion in human hepatoma and primary hepatocyte cells
Authors: PK Yadav, P Haruehanro, S Irani, T Wang, A Ansari, J Sheng, MM Hussain
The Journal of Biological Chemistry, 2022-03-10;0(0):101813. 2022-03-10
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Plasmon-enhanced Quantitative Lateral Flow Assay for Femtomolar Detection of SARS-CoV-2 Antibodies and Antigens
Authors: R Gupta, P Gupta, Z Wang, A Seth, J Morrissey, I George, S Gandra, G Storch, B Parikh, G Genin, S Singamanen
Research square, 2022-02-21;0(0):. 2022-02-21
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Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive Model
Authors: KT Liu, YN Gong, CG Huang, PN Huang, KY Yu, HC Lee, SC Lee, HJ Chiang, YA Kung, YT Lin, MJ Hsiao, PW Huang, SY Huang, HT Wu, CC Wu, RL Kuo, KF Chen, CT Hung, KY Oguntuyo, CS Stevens, S Kowdle, HP Chiu, B Lee, GW Chen, SR Shih
mSphere, 2022-02-02;7(1):e0088321. 2022-02-02
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Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
Nature, 2021-11-03;0(0):. 2021-11-03
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Acetylation of Calmodulin Regulates Synaptic Plasticity and Fear Learning
Authors: HL Zhang, B Zhao, W Han, YB Sun, P Yang, Y Chen, D Ni, J Zhang, DM Yin
The Journal of Biological Chemistry, 2021-07-31;0(0):101034. 2021-07-31
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Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein
Authors: CD Hodge, DJ Rosenberg, M Wilamowski, A Joachimiak, GL Hura, M Hammel
bioRxiv : the preprint server for biology, 2021-01-13;0(0):. 2021-01-13
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Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
Authors: L Kang, S Smith, C Wang
Journal of colloid and interface science, 2020-12-24;588(0):101-109. 2020-12-24
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TRI microparticles prevent inflammatory arthritis in a collagen-induced arthritis model
Authors: EJ Bassin, AR Buckley, JD Piganelli, SR Little
PLoS ONE, 2020-09-23;15(9):e0239396. 2020-09-23
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The Effect of Resveratrol or Curcumin on Head and Neck Cancer Cells Sensitivity to the Cytotoxic Effects of Cisplatin
Authors: M Bostan, GG Petric?-Ma, N Radu, R Hainarosie, CD Stefanescu, CC Diaconu, V Roman
Nutrients, 2020-08-26;12(9):. 2020-08-26
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Versatile, Multivalent Nanobody Cocktails Efficiently Neutralize SARS-CoV-2
Authors: Y Xiang, S Nambulli, Z Xiao, H Liu, Z Sang, WP Duprex, D Schneidman, C Zhang, Y Shi
bioRxiv, 2020-08-25;0(0):. 2020-08-25
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Utility of Centrifugation-Controlled Convective (C3) Flow for Rapid On-chip ELISA
Authors: W Espulgar, T Tadokoro, E Tamiya, M Saito
Sci Rep, 2019-12-27;9(1):20150. 2019-12-27
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Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
Int J Nanomedicine, 2019-08-01;14(0):6117-6131. 2019-08-01
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Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats
Authors: M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita
J. Neurosci., 2019-07-25;0(0):. 2019-07-25
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Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
J Clin Med, 2019-07-15;8(7):. 2019-07-15
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Targeting a Pre-existing Anti-transgene T Cell Response for Effective Gene Therapy of MPS-I in the Mouse Model of the Disease
Authors: G Squeri, L Passerini, F Ferro, C Laudisa, D Tomasoni, F Deodato, MA Donati, S Gasperini, A Aiuti, ME Bernardo, B Gentner, L Naldini, A Annoni, A Biffi, S Gregori
Mol. Ther., 2019-04-19;0(0):. 2019-04-19
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Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis
Authors: H Lin, R Muramatsu, N Maedera, H Tsunematsu, M Hamaguchi, Y Koyama, M Kuroda, K Ono, M Sawada, T Yamashita
EBioMedicine, 2017-12-07;0(0):. 2017-12-07
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YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation
Authors: M Martinez-G, KGK Goh, E Rackaityte, C Sakamoto, B Audrain, DG Moriel, M Totsika, JM Ghigo, MA Schembri, C Beloin
Sci Rep, 2017-09-12;7(1):11326. 2017-09-12
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Vitronectin as a Micromanager of Cell Response in Material-Driven Fibronectin Nanonetworks
Authors: M Cantini, K Gomide, V Moulisova, C González-G, M Salmerón-S
Adv Biosyst, 2017-08-10;1(9):1700047. 2017-08-10
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IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
JCI Insight, 2017-05-18;2(10):. 2017-05-18
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Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution
Authors: Foteini Kakulas
Nutrients, 2016-11-08;8(11):. 2016-11-08
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Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
Authors: Elodie Weider
J Biol Chem, 2016-06-08;0(0):. 2016-06-08
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Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
PLoS ONE, 2015-01-06;10(1):e0116772. 2015-01-06
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The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
Authors: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P
PLoS Pathog, 2013-12-26;9(12):e1003852. 2013-12-26
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Soluble beta-amyloid Precursor Protein Alpha binds to p75 neurotrophin receptor to promote neurite outgrowth.
Authors: Hasebe N, Fujita Y, Ueno M, Yoshimura K, Fujino Y, Yamashita T
PLoS ONE, 2013-12-16;8(12):e82321. 2013-12-16
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Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum.
Authors: Taylor H, Barua N, Bienemann A, Wyatt M, Castrique E, Foster R, Luz M, Fibiger C, Mohr E, Gill S
PLoS ONE, 2013-03-20;8(3):e56186. 2013-03-20
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Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010-02-16;5(2):e9246. 2010-02-16
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First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
Authors: Thadhani R, Powe CE, Tjoa ML
Diabetes Care, 2009-12-10;33(3):664-9. 2009-12-10
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Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta.
Authors: Palmer JC, Baig S, Kehoe PG, Love S
Am. J. Pathol., 2009-06-18;175(1):262-70. 2009-06-18
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Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
Authors: Kim JY, Kwok SK, Hur KH, Kim HJ, Kim NS, Yoo SA, Kim WU, Cho CS
Clin. Exp. Immunol., 2008-03-18;152(2):328-35. 2008-03-18
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Nanobacteria are mineralo fetuin complexes.
Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
PLoS Pathog., 2008-02-08;4(2):e41. 2008-02-08
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Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
Chest, 2007-05-15;132(1):58-63. 2007-05-15
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Used stop solution to read the plate and it works perfect
Perfect for stopping the action of HRP on TMB in ELISA