Anti- Cetuximab (Anti-Idiotype) Antibody

Detection of Cetuximab by Western Blot. Western blot shows Cetuximab, Rituximab, Brentuximab, and Gemtuzumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cetuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Detection of anti-EGFR/ErbB1 (Cetuximab) on A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with anti-EGFR/ErbB1 (Cetuximab) (Catalog # MAB9577), then anti-Cetuximab (Anti-Idiotype) (Catalog # MAB9626, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Specificity of Anti-Cetuximab (Anti-Idiotype) Antibody in Direct ELISA A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Cetuximab (Anti-Idiotype) Antibody (Catalog # MAB9626, 333 ng/mL) detected Cetuximab. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Detection of Cetuximab by Simple Western^TM. Simple Western lane view shows Cetuximab and Rituximab biosimilars, loaded at 0.2 mg/mL. A specific band was detected for Cetuximab at approximately 174 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626). This experiment was conducted under non-reducing conditions and using the 66-440 kDa separation system.