Anti- Trastuzumab (Anti-Idiotype) Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibodies
Catalog # Availability Size / Price Qty
FAB95471R-100UG
Detection of Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody on MCF-7 human cell line by flow cytometry.
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Citations (1)
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Anti- Trastuzumab (Anti-Idiotype) Alexa Fluor® 647-conjugated Antibody Summary

Specificity
Detects Trastuzumab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2261E
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Full-length Trastuzumab
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody on MCF-7 human cell line antibody by Flow Cytometry. View Larger

Detection of Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody on MCF-7 human cell line by flow cytometry. MCF-7 Human cell line was stained with Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody (Catalog # MAB9589), then Anti- Trastuzumab (Anti-Idiotype) Alexa Fluor® 647-conjugated Antibody (Catalog # FAB95471R, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # IC1051R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Trastuzumab

The anti-Trastuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Trastuzumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity.

Alternate Names
Trastuzumab

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Anti- Trastuzumab (Anti-Idiotype) Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Contextual reprogramming of CAR-T cells for treatment of HER2+ cancers
    Authors: Z Yang, L Li, A Turkoz, P Chen, R Harari-Ste, M Bobbin, O Stefanson, H Choi, V Pietrobon, B Alphson, A Goswami, V Balan, A Kearney, D Patel, J Yang, D Inel, V Vinod, A Cesano, B Wang, KH Roh, LS Qi, FM Marincola
    Journal of Translational Medicine, 2021-11-07;19(1):459.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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