Anti- Trastuzumab (Anti-Idiotype) Antibody
Anti- Trastuzumab (Anti-Idiotype) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Trastuzumab by Western Blot. Western blot of Centuximab (negative control), Rituximab (negative control), Trastuzumab, and Teprotumumab (negative control) biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Trastuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB95471) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Trastuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Trastuzumab (Anti-ErbB2/Her2) on MCF-7 human cell line by flow cytometry. MCF-7 Human cell line was stained with Human Trastuzumab (anti-Human ErbB2/Her2) Monoclonal Antibody (MAB9589), then Anti-Trastuzumab (Anti-Idiotype) (Catalog # MAB95471, filled histogram) or Rabbit IgG Isotype Control Antibody (MAB1050, open histogram) followed by APC-conjugated anti-rabbit IgG secondary antibody (F0111).
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Specificity of Anti-Trastuzumab (Anti-Idiotype) Antibody in Direct ELISA. A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Trastuzumab (Anti-Idiotype) Antibody (Catalog # MAB95471, 333 ng/mL) detected Trastuzumab. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab was observed.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trastuzumab
The anti-Trastuzumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Trastuzumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.
Product Datasheets
Citations for Anti- Trastuzumab (Anti-Idiotype) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera
Authors: Jason T. Smith, Alena Rudkouskaya, Shan Gao, Juhi M. Gupta, Arin Ulku, Claudio Bruschini et al.
Optica
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Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug–Target Engagement
Authors: Marien Ochoa, Alena Rudkouskaya, Jason T. Smith, Xavier Intes, Margarida Barroso
Methods in Molecular Biology
FAQs
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What is an anti-idiotype antibody?
An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody. We have anti-idiotype antibodies to commecially available therapeutic antibodies or biosimilars.
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