Home / BoNT-A Light Chain / C. botulinum BoNT-A Light Chain Antibody

C. botulinum BoNT-A Light Chain Antibody

Catalog #: MAB4489 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4489
MAB4489-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

C. botulinum BoNT-A Light Chain Antibody Summary

Species Reactivity
C. botulinum
Specificity
Detects C. botulinum BoNT‑A Light Chain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with BoNT-B light chain, BoNT-E light chain, BoNT-A heavy chain, BoNT-C1 heavy chain, or BoNT-D heavy chain is observed.
Source
Monoclonal Mouse IgG2A Clone # 503013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant Clostridium BoNT-A Light Chain
Met1-Phe425
Accession # Q45894
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant C. botulinum BoNT-A Light Chain (Catalog # 4489-ZN)
Immunoprecipitation
25 µg/mL
Cell lysates spiked with Recombinant C. botulinum BoNT-A Light Chain (Catalog # 4489-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-A Light Chain

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2‑4). E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

References
  1. Willems, A. et al. (1993) Res. Microbiol. 144:547.
  2. Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.
  3. Turton, K., et al. (2002) Trends. Biochem. Sci. 27:552.
  4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.
Long Name
Botulinum Neurotoxin Type A Light Chain
Entrez Gene IDs
5185061 (C. botulinum)
Alternate Names
BoNTA Light Chain; BoNT-A Light Chain

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