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Canine IL-4 Biotinylated Antibody

Catalog #: BAF754
6 Citations Datasheet / COA / SDS
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BAF754
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Citations (6)
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Canine IL-4 Biotinylated Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine IL-4 in Western blots. In this format, approximately 25% cross-reactivity with rhIL-4 is observed, 10% cross-reactivity with rpIL-4 is observed and less than 1% cross-reactivity with rmIL-4 and rrIL-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant canine IL-4 (R&D Systems, Catalog # 754-CL)
His25-His132
Accession # AAD11563
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Antigen Affinity-purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Canine IL‑4 (Catalog # 754-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa - 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1 - 3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Canine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature canine IL-4 shares 81%, 61%, 46%, 38%, and 37% aa sequence identity with feline, bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non‑hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8 - 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Khatlani, T. et al. (1999) J. Vet. Med. Sci. 61:967.
  4. van der Kaaij, S.Y. et al. (1999) Immunogenetics 49:142.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

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Citations for Canine IL-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge
    PLoS ONE, 2016-08-24;11(8):e0161169.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  2. Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs
    PLoS Negl Trop Dis, 2016-05-25;10(5):e0004614.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  3. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
    Authors: Costa-Pereira C, Moreira M, Soares R, Marteleto B, Ribeiro V, Franca-Dias M, Cardoso L, Viana K, Giunchetti R, Martins-Filho O, Araujo M
    BMC Vet Res, 2015-04-11;11(0):92.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  4. Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial.
    Authors: Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G
    Vaccine, 2007-03-15;25(21):4223-34.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
    Authors: Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM
    Vaccine, 2005-08-15;24(4):501-9.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes.
    Authors: Lemesre JL, Holzmuller P, Cavaleyra M, Goncalves RB, Hottin G, Papierok G
    Vaccine, 2005-04-22;23(22):2825-40.
    Species: Canine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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