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CellXVivo Human B Cell Expansion Kit

Catalog #: CDK005
7 Citations 1 Review Datasheet / COA / SDS
For the proliferative expansion of human B cell populations
Catalog # Availability Size / Price Qty
CDK005
Light Microscopy of B Cells.
4 Images
Product Details
Procedure
Citations (7)
FAQs
Reviews (1)
Summary Product Datasheets Data Images Background

CellXVivo Human B Cell Expansion Kit Summary

Kit Summary

For the proliferative expansion of B cell populations.

Key Benefits

  • Yields a 3-5 fold expanded population of B cells
  • Provides optimized reagents needed to induce B cell proliferation
  • Utilizes validated and straightforward procedures
  • Does not require specialized instrumentation

 

Why Expand B Cells Ex Vivo?

B cells are a subset of lymphocytes that express immunoglobulins on their cell surface. The B cell population is a vital component of the humoral immune response. B cell activation leads to antigen uptake and presentation, affinity maturation and class switching of the immunoglobulins, and antibody secretion. B cells represent a small portion (~5%) of the peripheral lymphocyte population making it difficult to obtain sufficient B cell quantities for many research applications. The ability to expand B cell populations ex vivo is valuable to researchers who require B cells for downstream applications including high throughput B cell assays and ex vivo differentiation of T cells.

 

Kit Contents

This kit contains the following optimized reagents for the expansion of human B cell populations.

  • B Cell Expander Reagents
  • Reconstitution Buffers
  • Provides enough reagents for the 3-5 fold expansion of a starting population of 107 human B cells

Stability and Storage

Store the unopened kit at 2-8 °C. Do not use past the kit expiration date. Opened or reconstituted B Cell Expander 1, B Cell Expander 2, and B Cell Expander 3 should be stored at 2-8 °C under sterile conditions for up to 30 days or at -20 °C to -70 °C in a manual defrost freezer for up to 3 months.* Opened Reconstitution Buffer 1 and Reconstitution Buffer 2 should be stored at 2-8 °C under sterile conditions for up to 12 months.*

*Provided this is within the expiration date of the kit.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Product Datasheets

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Scientific Data

Cell Morphology Light Microscopy of B Cells. View Larger

Light Microscopy of B Cells. B cells were imaged at 20X magnification following isolation from PMBCs and (B) after 5 days in culture using reagents included in the Human B Cell Expansion Kit.

Flow Cytometry Detection of CD20 in Human B Cells. View Larger

Detection of CD20 in Human B Cells. Human B cells were expanded for 5 days using reagents included in the Human B Cell Expansion Kit. The cells were labeled with a PE-conjugated Mouse Anti-Human CD20 Monoclonal Antibody (R&D Systems, Catalog # FAB4225P; filled histogram) or a PE-conjugated Mouse IgG1 Isotype control (R&D Systems, Catalog # IC002P; open histogram).

Cell Differentiation/Maturation Human B Cell Expansion.  View Larger

Human B Cell Expansion.  Human B cells were isolated from PMBCs and cultured for 5 days using reagents included in the Human B Cell Expansion Kit. B cell expansion was measured with Resazurin (R&D Systems, Catalog # AR002).

Assay Procedure

Refer to the product datasheet for complete product details.

 

Briefly, human B cell populations can be expanded using the following procedure:

  • Isolate human B cells from PBMC preparation
  • Culture B cells in B Cell Expander-supplemented media
  • Measure B cell expansion using Resazurin

View Graphic Procedure
 
Reagents Provided

Reagents Supplied in the CellXVivo™ Human B Cell Expansion Kit (Catalog # CDK005):

  • B Cell Expander 1
  • B Cell Expander 2
  • B Cell Expander 3
  • Reconstitution Buffer 1
  • Reconstitution Buffer 2

 

Other Supplies Required

Reagents

  • MagCellect™ Human B Cell Isolation Kit (R&D Systems, Catalog # MAGH103, or equivalent).
  • Resazurin (R&D Systems, Catalog # AR002, or equivalent).
  • Ficoll-Hypaque™
  • RPMI 1640
  • L-Glutamine
  • Penicillin
  • Streptomycin
  • Fetal Bovine Serum (FBS)
  • beta-Mercaptoethanol

Equipment

  • Tissue culture flasks and/or plates
  • Inverted microscope
  • Hemocytometer
  • 37 °C and 5% CO2 humidified cell culture incubator
  • Benchtop centrifuge
  • Pipettes and pipette tips

 

Procedure Overview

R&D Systems Protocol for Human B Cell expansion

Isolate PBMCs from human blood.

Isolate PBMCs from human blood

Isolate human B cells from a PBMC preparation (e.g., using magnetic cell selection).

Isolate human B Cells from PBMCs

Perform a cell count.

Perform a cell count

Culture 2 x 105 cells/mL in media supplemented with reconstituted B Cell Expanders 1-3.

Incubate the cells for 5 days.

Measure B cell expansion using Resazurin.

Culture cells in media

Citations for CellXVivo Human B Cell Expansion Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Targeting CD74 in B-cell non-Hodgkin lymphoma with the antibody-drug conjugate STRO-001
    Authors: X Li, C Abrahams, A Yu, M Embry, R Henningsen, V DeAlmeida, S Matheny, T Kline, A Yam, R Stafford, T Hallam, M Lupher, A Molina
    Oncotarget, 2023-01-12;14(0):1-13.  2023-01-12
  2. The Rationale for the Dual-Targeting Therapy for RSK2 and AKT in Multiple Myeloma
    Authors: R Isa, M Horinaka, T Tsukamoto, K Mizuhara, Y Fujibayash, Y Taminishi-, H Okamoto, S Yasuda, Y Kawaji-Kan, Y Matsumura-, S Mizutani, Y Shimura, M Taniwaki, T Sakai, J Kuroda
    International Journal of Molecular Sciences, 2022-03-08;23(6):.  2022-03-08
  3. Tumor-B-cell interactions promote isotype switching to an immunosuppressive IgG4 antibody response through upregulation of IL-10 in triple negative breast cancers
    Authors: NJ Toney, LM Opdenaker, K Cicek, L Frerichs, CR Kennington, S Oberly, H Archinal, R Somasundar, J Sims-Mourt
    Journal of Translational Medicine, 2022-03-07;20(1):112.  2022-03-07
  4. IgA transcytosis and antigen recognition govern ovarian cancer immunity
    Authors: S Biswas, G Mandal, KK Payne, CM Anadon, CD Gatenbee, RA Chaurio, TL Costich, C Moran, CM Harro, KE Rigolizzo, JA Mine, J Trillo-Tin, N Sasamoto, KL Terry, D Marchion, A Buras, RM Wenham, X Yu, MK Townsend, SS Tworoger, PC Rodriguez, AR Anderson, JR Conejo-Gar
    Nature, 2021-02-03;0(0):.  2021-02-03
  5. B cell-specific XIST complex enforces X-inactivation and restrains atypical B cells
    Authors: B Yu, Y Qi, R Li, Q Shi, A Satpathy, HY Chang
    bioRxiv : the preprint server for biology, 2021-01-05;0(0):.  2021-01-05
  6. BAFF-driven NLRP3 inflammasome activation in B cells
    Authors: KH Lim, LC Chen, K Hsu, CC Chang, CY Chang, CW Kao, YF Chang, MC Chang, CG Chen
    Cell Death Dis, 2020-10-01;11(9):820.  2020-10-01
  7. A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice
    Authors: G Yi, X Xu, S Abraham, S Petersen, H Guo, N Ortega, P Shankar, N Manjunath
    EBioMedicine, 2017-10-06;25(0):87-94.  2017-10-06

FAQs

  1. Can the CellXVivo Human B Cell Expansion Kit, catalog # CDK005, be used for expansion of memory B cells?

    • Based on the components used in the CDK005 kit, the kit is expected to expand total B cells and is not specialized for memory B cells.

  2. Will ExCellerate B Cell Media, Xeno-Free, Catalog # CCM031, promote expansion of B cell clones without addition of growth factors?

    • Catalog # CCM031 must be supplemented with growth factors in order to use it for expansion of B cell clones. Information on appropriate growth factor cocktails are available in published literature. Here are two references:

      1. LeBien, T.W. and T.F. Tedder. (2008) Blood 112:1570.

      2. Basso, K. and R. Dalla-Favera (2015) Nat. Rev. Immunol. 15:172.

  3. Can CellXVivo Human B cells expansion kit, Catalog # CDK005, also promote B cell Maturation (differentiation into plasma cells)?

    • Catalog # CDK005 has only been tested for B cell expansion. This kit has not been tested for B cell Maturation.

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CellXVivo Human B Cell Expansion Kit
By Leslie Priddy on 04/04/2018