CometAssay Single Cell Gel Electrophoresis Assay-Silver Summary
Includes CometAssay reagents and components for the convenient silver staining of single cell gel electrophoresis samples.Key Benefits
• Allows visualization by standard light microscopy
• Permanent staining for archiving samples
• Optimized for CometSlide staining
Designed specifically for use with the CometSlides to minimize unwanted background and the amount of hazardous waste generated by silver nitrate. The silver staining components may be purchased separately.
Product Specifications
CometAssay and FLARE
NOTE:
Once reconstituted 2X Staining Reagent #4 is stable for 3 months at 4°C.
Kit Contents
• CometAssay Lysis Solution
• CometAssay LMAgarose
• CometSlides (2 Well, 25 Slides)
• 200 mM EDTA, pH 10
• 20X Staining Reagent #1
• 20X Staining Reagent #2
• 20X Staining Reagent #3
• 2X Staining Reagent #4
• 10X Fixation Additive
Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
FAQs
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How long can CometSlides™ be stored after electrophoresis?
Prior to staining, slides immersed in 70% ethanol for 5 minutes and then dried can be stored at room temperature for 1 year prior to staining. Permanent records can be created after staining for visualization by standard light microscopy using the CometAssay® Silver Staining Kit (Catalog # 4251-050-K).
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What can cause observation of artefacts during imaging of Comet data?
For optimal imaging, all cells should be in the same plane and this is achieved by completely drying the agarose. Artefacts may be introduced if the source cells used for analyis has any debris. The debris should be allowed to settle before pipetting cells. The LMagarose can also be the cause of artefacts. To prevent debris from accumulating in the agar, melt it at 80-90 °C so it is runny, spin it at low speed and aliquot into sterile tubes in a hood. Use each aliquot for a single assay.
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