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Cultrex ReadyBME, Stem Cell Qualified, Reduced Growth Factor

Catalog #: 3434-050-RTU
3 Citations Datasheet / COA / SDS
Pre-diluted Cultrex Stem Cell-Qualified RGF Basement Membrane Extract
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3434-050-RTU
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Cultrex ReadyBME, Stem Cell Qualified Reduced Growth Factor_3434-050-RTU
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Cultrex ReadyBME, Stem Cell Qualified, Reduced Growth Factor Summary

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Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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N/A Maintenance of Human Induced Pluripotent Stem Cells_Cultrex ReadyBME_3434-050-RTU View Larger

Maintenance of Human Induced Pluripotent Stem Cells. JOY6 human induced pluripotent stem cell (hiPSC) colonies were successfully cultured for > 10 passages using Cultrex ReadyBME. A) Representative image of JOY6 hiPSC colonies cultured on Cultrex ReadyBME. B) hiPSCs cultured on Cultrex ReadyBME retain stemness markers as shown by flow cytometry using a Human/Mouse Oct-3/4 APC-conjugated Antibody (Catalog # IC1759A) and Human/Mouse SSEA-4 Fluorescein-conjugated Antibody (Catalog # FAB1435F). C) hiPSCs remain negative for SSEA-1expression as detected using a Human/Mouse SSEA-1 PerCP-conjugated Antibody (Catalog # FAB2155C).

N/A Human iPSC and ESC Colony Formation_Cultrex ReadyBME_3434-050-RTU View Larger

Colony Formation of Human iPSC and ESC Cultured using Cultrex ReadyBME. Three human induced pluripotent stem cell lines (IPSK3, IBJ6, and JOY6) and one human embryonic stem cell line (BG01V) were cultured using Cultrex ReadyBME. Representative images of colonies for each of the pluripotent stem cell lines shows normal colony formation. BG01V human embryonic stem cells are licensed for ViaCyte, Inc.

N/A Trilineage Differentiation of Human Pluripotent Stem Cells using Cultrex ReadyBME View Larger

Differentiation of Human Induced Pluripotent Stem Cells. IBJ6 human induced pluripotent stem cells were cultured for 6 passages using Cultrex ReadyBME. Pluripotency was tested using the Human Pluripotent Stem Cell Functional Identification Kit (Catalog # SC027B), which differentiates hiPSCs into the three germ layers.Immunocytochemistry showed that IBJ6 hiPSC successfully differentiated into endoderm (SOX17), mesoderm (Brachyury),and ectoderm (Otx2). Cells were counterstained with DAPI (Catalog # 5748).

N/A Cultrex ReadyBME Similar Performance to Stem Cell Qualified Cultrex RGF BME View Larger

Cultrex ReadyBME Performs Equal to Cultrex Stem Cell Qualified RGF BME. Human induced pluripotent stem cells (iPSCs) were maintained in culture using Cultrex ReadyBME or Stem Cell Qualified (SCQ) Cultrex RGF BME (Catalog # 3434-005-02) and evaluated for (A) expression of stemness markers, SSEA-4 and Oct-3/4, and (B) differentiation capacity into endoderm. A) iPSCs cultured using Cultrex ReadyBME showed similar expression of SSEA-4 and Oct-3/4 compared to SCQ Cultrex RGF BME. B) iPSC cultured using Cultrex ReadyBME showed similar efficiency to differentiate into endoderm, as determined by SOX17 expression, compared to cells grown using SCQ Cultrex RGF BME

Citations for Cultrex ReadyBME, Stem Cell Qualified, Reduced Growth Factor

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3 Citations: Showing 1 - 3
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  1. NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis
    Authors: D Ilter, S Drapela, T Schild, NP Ward, E Adhikari, V Low, J Asara, T Oskarsson, EK Lau, GM DeNicola, MR McReynolds, AP Gomes
    Redox Biology, 2023-02-09;61(0):102627.  2023-02-09
  2. Glucose deprivation promotes pseudo-hypoxia and de-differentiation in lung adenocarcinoma
    Authors: P Saggese, A Pandey, E Fung, A Hall, J Yanagawa, EF Rodriguez, TR Grogan, G Giurato, G Nassa, A Salvati, A Weisz, SM Dubinett, C Scafoglio
    bioRxiv : the preprint server for biology, 2023-02-01;0(0):.  2023-02-01
  3. Osteocyte Vegf-a contributes to myeloma-associated angiogenesis and is regulated by Fgf23
    Authors: PL Mulcrone, SKE Edwards, DN Petrusca, LS Haneline, J Delgado-Ca, GD Roodman
    Sci Rep, 2020-10-14;10(1):17319.  2020-10-14

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