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Cultrex Stem Cell Qualified Laminin I, Pathclear

Catalog #: 3400-010-03 Datasheet / COA / SDS
Mouse Laminin I specifically qualified for use in stem cell applications
Catalog # Availability Size / Price Qty
3400-010-03
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Cultrex Stem Cell Qualified Laminin I, Pathclear Summary

Cultrex Stem Cell Qualified Laminin I provides a functionally defined and effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in an undifferentiated state.
 

Key Benefits

• Qualified to support pluripotent stem cell expansion and differentiation
• Pathclear-tested to ensure a pathogen and virus-free material
• Source tissue is LDEV-free

Why Use Cultrex Stem Cell Qualified Laminin I?

Cultrex Mouse Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. This highly purified preparation of mouse Laminin I is composed of alpha 1 beta 1 gamma 1 chains with a total molecular weight (MW) of 800 KDa.

Negative for the presence of bacteria and fungi.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
1 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Testing Cell Culture
Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnostic use.

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FAQs

  1. What is the recommended working concentration for Cultrex Laminin I?

    • The recommended working concentration for thin coating is 0.05-10 µg/cm2 . However, conditions must be optimized for each cell line or model. Cultrex Laminin I should be used at 6 mg/mL for 3-D culture applications.

       

  2. What is Laminin I?

    • Laminin I is a major component of extracellular matrix. Cultrex Laminin I is purified from murine EHS sarcoma. It is composed of α1β1γ1 chains with a total MW of 800,000 Da. Cultrex Laminin I increases cell adhesion, migration, growth, differentiation, neurite outgrowth, protease production, and malignancy. The response is dependent on cell type.

  3. Can Cultrex Mouse Laminin I be used on glass cover slips?

    • Coating glass cover slips with Cultrex Laminin I is quite common and has been published extensively.

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